Widespread density dependence of bacterial growth under acid stress

Benefits of cooperation intrinsically depend on density because biological interaction requires organismal proximity. Microbial cooperative traits are common, yet systematic tests for a shared cooperative phenotype across diverse species are rare, as are direct tests for the Allee effect - positive density dependence of fitness. Here we test for positive density dependence of growth under acid stress in five phylogenetically widespread bacterial species - three Gram-negative and two Gram-positive - and find the Allee effect in all five. However, social protection from acid stress appears to have evolved by different mechanisms across species. In Myxococcus xanthus, the acid-stress Allee effect is found to be mediated by pH-regulated secretion of a diffusible molecule present in supernatants of high-density cultures. In contrast, growth from low density under acid stress by the other species was not enhanced by high-density supernatant. Additionally, density dependence of Myxococcus fruiting-body formation during starvation is found to increase with acid stress, suggesting that abiotic stresses other than starvation shape the evolution of aggregative development. Our findings suggest that high cell density may protect against acid stress in most bacterial species and in Myxococcus may promote predation on microbes that acidify their local environment by secretion of metabolic byproducts.

Positive density dependence acting on mortality can help maintain species-rich communities

Conspecific negative density dependence is ubiquitous and has long been recognized as an important factor favoring the coexistence of competing species at local scale. By contrast, a positive density-dependent growth rate is thought to favor species exclusion by inhibiting the growth of less competitive species. Yet, such conspecific positive density dependence often reduces extrinsic mortality (e.g. reduced predation), which favors species exclusion in the first place. Here, using a combination of analytical derivations and numerical simulations, I show that this form of positive density dependence can favor the existence of equilibrium points characterized by species coexistence. Those equilibria are not globally stable, but allow the maintenance of species-rich communities in multispecies simulations. Therefore, conspecific positive density dependence does not necessarily favor species exclusion. On the contrary, some forms of conspecific positive density dependence may even help maintain species richness in natural communities. These results should stimulate further investigations into the precise mechanisms underlying density dependence.

Soil fungal networks maintain local dominance of ectomycorrhizal trees

The mechanisms regulating community composition and local dominance of trees in species-rich forests are poorly resolved, but the importance of interactions with soil microbes is increasingly acknowledged. Here, we show that tree seedlings that interact via root-associated fungal hyphae with soils beneath neighbouring adult trees grow faster and have greater survival than seedlings that are isolated from external fungal mycelia, but these effects are observed for species possessing ectomycorrhizas (ECM) and not arbuscular mycorrhizal (AM) fungi. Moreover, survival of naturally-regenerating AM seedlings over ten years is negatively related to the density of surrounding conspecific plants, while survival of ECM tree seedlings displays positive density dependence over this interval, and AM seedling roots contain greater abundance of pathogenic fungi than roots of ECM seedlings. Our findings show that neighbourhood interactions mediated by beneficial and pathogenic soil fungi regulate plant demography and community structure in hyperdiverse forests.

The winner takes it all: how semelparous insects can become periodical

The aim of this short note is to give a simple explanation for the remarkable periodicity of Magicicada species, which appear as adults only every 13 or 17 years, depending on the region. We show that a combination of two types of density dependence may drive, for large classes of initial conditions, all but one year class to extinction. Competition for food leads to negative density dependence in the form of a uniform (i.e., affecting all age classes in the same way) reduction of the survival probability. Satiation of predators leads to positive density dependence within the reproducing age class. The analysis focuses on the full life cycle map derived by iteration of a semelparous Leslie matrix.

Pinned, locked, pushed, and pulled traveling waves in structured environments

Traveling fronts describe the transition between two alternative states in a great number of physical and biological systems. Examples include the spread of beneficial mutations, chemical reactions, and the invasions by foreign species. In homogeneous environments, the alternative states are separated by a smooth front moving at a constant velocity. This simple picture can break down in structured environments such as tissues, patchy landscapes, and microfluidic devices. Habitat fragmentation can pin the front at a particular location or lock invasion velocities into specific values. Locked velocities are not sensitive to moderate changes in dispersal or growth and are determined by the spatial and temporal periodicity of the environment. The synchronization with the environment results in discontinuous fronts that propagate as periodic pulses. We characterize the transition from continuous to locked invasions and show that it is controlled by positive density-dependence in dispersal or growth. We also demonstrate that velocity locking is robust to demographic and environmental fluctuations and examine stochastic dynamics and evolution in locked invasions.