Pengaruh Media pada Karakter Biokimia dan Keberhasilan Pencangkokan Tanaman Teh (Camellia sinensis L. (O.) Kuntze) pada Klon TRI 2025

The large number of old, damaged, or dead tea plants cause the plant population per hectare to be low that eventually decreases the productivity. Propagation using the transplantation technique is used for embroidery because it has the advantage of a shorter immature plant period and higher seed size so that it can compensate for the surrounding plants but the weakness of transplantation is low success rate. This study aims to 1) determine the morphological changes of root transplantation and biochemistry on TRI 2025 clone transplantation using cocopeat, husk charcoal, and moss, 2) determine the success rate of transplantation in each medium. This research was conducted in August 2018–January 2019 at the Pagilaran production garden, PT. Pagilaran, Batang, Central Java. Field experiments were prepared using a single factor Completely Randomized Environment Design (CRD). The factors tested were the type of transplantation media, namely the media of husk charcoal, cocopeat, and moss. Observations were made on transplantation root morphology, levels of glucose, sucrose, and total sugar in the transplanted stem bark and the concentration of auxin, gibberellin, and cytokinin in the transplant roots. The data obtained were then analyzed using variance and if there was a significant difference between treatments, the Duncan multiple distance test (DMRT) was continued at the 95% confidence level. The results showed that the type of media had no significant effect on the ability to form glucose, sucrose, and total sugar in the bark. The development of root morphology of cocopeat media was seen to be the highest. The ratio of cytokinin/auxin in cocopeat was the lowest but the highest levels of cytokinin and auxin were found in moss, while cocopeat had the highest level of gibberellin. The highest transplanting success rate was found in moss medium with 58.33%, cocopeat and husk charcoal media had the same success rate, namely 41.60%. Keywords: transplantation, biochemistry, media, success, TRI 2025

Transplantation Principles

Transplantation is a truly multidisciplinary specialty where a surgical procedure requires inputs from both, the specialists of the specific organ e.g. nephrologist for kidney transplant or hepatologist for liver transplant, as well as from experts of other specialties such as immunology, infectious diseases etc. It is also a rapidly grooving field with the advances in surgical techniques, immunological knowledge and pharmacology. A recent, major advance in the field of transplantation is the emergence of new procedure of vascularized composite allotransplantation, which includes transplantation of non-lifesaving organs such as face, hands, abdominal wall or uterus. Additionally, organ shortage in transplantation yielded a new area of research such as xenotransplantation and regenerative medicine. The specialty of transplantation may be difficult to comprehend for those entering the field; thus, the goal of this chapter is to provide a comprehensive overview of the most important aspects of transplantation. This review contains 2 figures, 3 tables, and 67 references. Keywords: transplantation, solid organ transplantation (SOT), vascularized composite allotransplantation (VCA), transplant immunology, allorecognition, acute rejection, chronic transplant rejection, principles of transplantation, transplantation terminology, immunosuppressive drugs

Perception Of The Nurse In The Process Of Donation Of Organs And Fabrics For Transplantation

Objective: to know the nurses' perception in the process of organ and tissue donation for transplants. Methods: qualitative research with data collection performed through a semistructured interview with 16 nurses from a hospital. The data were submitted to Bardin content analysis. Results: categories emerged after content analysis were as follows: Organ donation process: nurses' experience; Nursing care for potential donors; Family approach; Main difficulties in the donation process. Conclusion: the research demonstrated the real difficulties of the professionals during the donation process, such as lack of human resources, extensive protocols, and lack of awareness of the society to understand the donation process and the family approach. Keywords: Transplantation of Organs; Obtaining Tissues and Organs; Nursing care.

Testicular cell transplantation of neon tetra Paracheirodon innesi into common carp fry

<p class="NoParagraphStyle" align="center"><strong>ABSTRACT</strong></p><p class="NoParagraphStyle" align="center"><strong> </strong></p><p class="NoParagraphStyle">Neon tetra <em>Paracheirodon innesi</em> is an ornamental fish that have high export value. However, production is still relatively low due to low fecundity (approximately 180 eggs/female). Technology of testicular cell transplantation of neon tetra as donor to common carp as recipient fish which have high fecundity provides a promising way to overcome problem of neon tetra production. This research was performed to determine the optimum age of common carp fry that is able to receive donor cells and allow high success of transplantation. In this research, the testes of neon tetra fish were dissociated by 0.5% trypsin solution. The testicular cells were labeled with PKH-26 fluorescent dye, and then transplanted into the peritoneal cavity of seven, ten, and 14 days post hatching common carp fry. The results showed that the survival of seven day-old transplanted fry (31.25%) was lower than that of ten day-old (37.75%) and 14 day-old transplanted fry (56.25%). Percentage of fish colonized testicular cells donor at 21 days post-transplantation on seven days old and ten days old fry were similar (80%), while on 14 day-old fry was 60%. Based on the cumulative transplantation success rate (survival and colonization rates), transplantation on 14 days old fry (33.75%) showed higher result compared to transplantation on seven days old fry (25.00%) and ten day-old fry (30.00%). It can be concluded that transplantation of neon tetra testicular cells to common carp fry have been successfully carried out, and the optimum age of common carp fry to transplantation was 14 days after hatching.</p><p class="NoParagraphStyle"> </p><p class="NoParagraphStyle">Keywords: transplantation, colonization, testicular cells, common carp, neon tetra</p><p class="NoParagraphStyle"> </p><p class="NoParagraphStyle"> </p><p class="NoParagraphStyle" align="center"><strong>ABSTRAK</strong></p><p class="NoParagraphStyle" align="center"><strong> </strong></p><p class="NoParagraphStyle">Ikan neon tetra <em>Paracheirodon innesi</em> merupakan ikan hias yang memiliki nilai ekspor yang tinggi. Namun demikian, tingkat produksinya masih relatif rendah karena fekunditas ikan neon tetra yang sedikit (sekitar 180 telur/induk). Teknologi transplantasi sel testikular ikan neon tetra (ikan donor) ke ikan mas yang memiliki fekunditas telur yang banyak dan diharapkan mampu mengatasi ketersediaan benih ikan neon tetra. Penelitian ini bertujuan untuk menentukan umur optimum benih ikan mas (calon ikan semang) yang mampu menerima sel donor dengan baik dan memiliki keberhasilan kolonisasi yang tinggi. Testis ikan neon tetra didisosiasi menggunakan larutan tripsin 0,5%. Sel testikular diwarnai dengan PKH-26, kemudian ditransplantasikan ke rongga peritoneal benih ikan mas umur tujuh, sepuluh, dan 14 hari setelah menetas. Hasil penelitian menunjukkan bahwa tingkat kelangsungan hidup ikan mas perlakuan transplantasi umur tujuh hari (31,25%) lebih rendah dibandingkan dengan perlakuan transplantasi umur sepuluh hari (37,50%) dan 14 hari (56,25%). Persentase ikan terkolonisasi sel donor pada hari ke-21 pascatransplantasi pada benih umur tujuh dan sepuluh hari adalah sama (80%), sedangkan transplantasi benih umur 14 hari sebesar 60%. Berdasarkan keberhasilan transplantasi secara kumulatif (tingkat kelangsungan hidup dan kolonisasi), transplantasi pada benih umur 14 hari (33,75%) menunjukkan hasil lebih tinggi dibandingkan dengan perlakuan transplantasi pada benih umur tujuh hari (25,00%) dan benih umur sepuluh hari (30,00%). Transplantasi sel testikular ikan neon tetra pada benih ikan mas telah berhasil dilakukan, dan umur optimum benih ikan mas adalah 14 hari setelah menetas.</p><p class="NoParagraphStyle"> </p><p class="NoParagraphStyle">Kata kunci: transplantasi, kolonisasi, sel testikular, ikan mas, ikan neon tetra</p><p> </p>