Biological and economic values of Dipterocarpaceae, the main timber forest product of Indonesia

Dipterocarpaceae is known as a very important tree family both biologically and economically. Its distribution around the world covers the areas of Peninsular Malaysia, the Philippines, Sumatra, Kalimantan, Java, Sulawesi, Maluku to Papua. Dipterocarpaceae family has a high economic value, such as producing wood, balsam, resin, charcoal, fat, fruit, bark, essential oil, and camphor. Its products have very important roles for domestic use and export needs. As representatives of Dipterocarpaceae, the economic value of Shorea Roxb. ex Gaertner f, Dipterocarpus Gaertner f, and Dryobalanops Gaertner f will be discussed. Considering the very important role of Dipterocarpaceae, both biologically and economically, it is necessary to handle it sustainably, through the following actions such as conservation of genetic resources, seed physiology, seed handling, seedling ecology, root symbiosis and nutrition, pest and disease, management of natural forest, and plantation, and also non-timber forest product from Dipterocarpaceae. Dipterocarpaceae dikenal sebagai famili pohon yang sangat penting baik secara biologis maupun ekonomis. Penyebarannya di seluruh dunia meliputi wilayah Semenanjung Malaysia, Filipina, Sumatera, Kalimantan, Jawa, Sulawesi, Maluku hingga Papua. Famili Dipterocarpaceae memiliki nilai ekonomi yang tinggi, seperti menghasilkan kayu, balsam, damar, arang, lemak, buah, kulit kayu, minyak atsiri, dan kapur barus. Produk-produknya memiliki peran yang sangat penting untuk kebutuhan domestik dan ekspor. Sebagai perwakilan Dipterocarpace, nilai ekonomi Shorea Roxb. ex Gaertner f, Dipterocarpus Gaertner f, dan Dryobalanops Gaertner f akan dibahas. Mengingat peranan Dipterocarpaceae yang sangat penting, baik secara biologis maupun ekonomis, maka perlu dilakukan penanganan secara berkelanjutan, melalui tindakan-tindakan seperti konservasi sumber daya genetik, fisiologi benih, penanganan benih, ekologi semai, simbiosis dan nutrisi akar, hama dan penyakit, pengelolaan hutan alam, dan perkebunan, serta hasil hutan bukan kayu dari Dipterocarpaceae.

Blumenols as shoot markers of root symbiosis with arbuscular mycorrhizal fungi

High-through-put (HTP) screening for functional arbuscular mycorrhizal fungi (AMF)-associations is challenging because roots must be excavated and colonization evaluated by transcript analysis or microscopy. Here we show that specific leaf-metabolites provide broadly applicable accurate proxies of these associations, suitable for HTP-screens. With a combination of untargeted and targeted metabolomics, we show that shoot accumulations of hydroxy- and carboxyblumenol C-glucosides mirror root AMF-colonization in Nicotiana attenuata plants. Genetic/pharmacologic manipulations indicate that these AMF-indicative foliar blumenols are synthesized and transported from roots to shoots. These blumenol-derived foliar markers, found in many di- and monocotyledonous crop and model plants (Solanum lycopersicum, Solanum tuberosum, Hordeum vulgare, Triticum aestivum, Medicago truncatula and Brachypodium distachyon), are not restricted to particular plant-AMF interactions, and are shown to be applicable for field-based QTL mapping of AMF-related genes.

Complexity of miRNA-dependent regulation in root symbiosis

The development of root systems may be strongly affected by the symbiotic interactions that plants establish with soil organisms. Legumes are able to develop symbiotic relationships with both rhizobial bacteria and arbuscular mycorrhizal fungi leading to the formation of nitrogen-fixing nodules and mycorrhizal arbuscules, respectively. Both of these symbiotic interactions involve complex cellular reprogramming and profound morphological and physiological changes in specific root cells. In addition, the repression of pathogenic defence responses seems to be required for successful symbiotic interactions. Apart from typical regulatory genes, such as transcription factors, microRNAs (miRNAs) are emerging as riboregulators that control gene networks in eukaryotic cells through interactions with specific target mRNAs. In recent years, the availability of deep-sequencing technologies and the development of in silico approaches have allowed for the identification of large sets of miRNAs and their targets in legumes . A number of conserved and legume-specific miRNAs were found to be associated with symbiotic interactions as shown by their expression patterns or actions on symbiosis-related targets. In this review, we combine data from recent literature and genomic and deep-sequencing data on miRNAs controlling nodule development or restricting defence reactions to address the diversity and specificity of miRNA-dependent regulation in legume root symbiosis. Phylogenetic analysis of miRNA isoforms and their potential targets suggests a role for miRNAs in the repression of plant defence during symbiosis and revealed the evolution of miRNA-dependent regulation in legumes to allow for the modification of root cell specification, such as the formation of mycorrhized roots and nitrogen-fixing nodules.