Variation in Petal and Leaf Wax Deposition Affects Cuticular Transpiration in Cut Lily Flowers

The vase life of cut flowers is largely affected by post-harvest water loss. Cuticular wax is the primary barrier to uncontrolled water loss for aerial plant organs. Studies on leaf cuticular transpiration have been widely conducted; however, little is known about cuticular transpiration in flowers. Here, the cuticular transpiration rate and wax composition of three lily cultivars were determined. The minimum water conductance of tepal cuticles was higher at the green bud than open flower stage. Lily cuticular transpiration exhibited cultivar- and organ-specific differences, where transpiration from the tepals was higher than leaves and was higher in the ‘Huang Tianba’ than ‘Tiber’ cultivar. The overall wax coverage of the tepals was higher compared to that of the leaves. Very-long-chain aliphatics were the main wax constituents and were dominated by n-alkanes with carbon (C) chain lengths of C27 and C29, and C29 and C31 in the tepal and leaf waxes, respectively. Primary alcohols and fatty acids as well as small amounts of alkyl esters, ketones, and branched or unsaturated n-alkanes were also detected in both tepal and leaf waxes, depending on the cultivar and organ. In addition, the chain-length distributions were similar between compound classes within cultivars, whereas the predominant C-chain lengths were substantially different between organs. This suggests that the less effective transpiration barrier provided by the tepal waxes may result from the shorter C-chain aliphatics in the tepal cuticle, compared to those in the leaf cuticle. These findings provide further insights to support the exploration of potential techniques for extending the shelf life of cut flowers based on cuticular transpiration barrier properties.

DO RADIOCARBON AGES OF PLANT WAX BIOMARKERS AGREE WITH 14C-TOC/OSL-BASED AGE MODELS IN AN ARID HIGH-ALTITUDE LAKE SYSTEM?

ABSTRACT To elucidate the dynamics of terrestrial leaf waxes in a high-altitude lake system, we performed compound-specific radiocarbon analysis (CSRA) of long-chain n-alkanes in two sediment core sections from Lake Karakul (Pamirs, Tajikistan) and in surface soil samples from the catchment area. We aimed to answer the question whether the n-alkanes are delivered into the lake sediment with substantial delay due to storage in soils, which may cause a potential bias when used as paleoenvironmental proxies. In the surface soils, the CSRA results reveal an age range of n-alkanes from modern to 2278 ± 155 cal BP. In the two sediment core samples, three of the four n-alkane ages fell on the lower ends of the 1σ-uncertainty ranges of modeled ages of the sediments (based on AMS 14C-TOC and OSL dating results). We conclude that sedimentary leaf waxes represent compounds with intermediate turnover time in soils, for example originating from alluvial plains close to the shores. Overall, the results provide evidence that sedimentary leaf wax compounds in this cold and arid setting are potentially older than the conventional age model indicates, but these findings need to be interpreted in context of the generally large uncertainty ranges of such age models.

A co-opted steroid synthesis gene, maintained in sorghum but not maize, is associated with a divergence in leaf wax chemistry

Virtually all land plants are coated in a cuticle, a waxy polyester that prevents nonstomatal water loss and is important for heat and drought tolerance. Here, we describe a likely genetic basis for a divergence in cuticular wax chemistry between Sorghum bicolor, a drought tolerant crop widely cultivated in hot climates, and its close relative Zea mays (maize). Combining chemical analyses, heterologous expression, and comparative genomics, we reveal that: 1) sorghum and maize leaf waxes are similar at the juvenile stage but, after the juvenile-to-adult transition, sorghum leaf waxes are rich in triterpenoids that are absent from maize; 2) biosynthesis of the majority of sorghum leaf triterpenoids is mediated by a gene that maize and sorghum both inherited from a common ancestor but that is only functionally maintained in sorghum; and 3) sorghum leaf triterpenoids accumulate in a spatial pattern that was previously shown to strengthen the cuticle and decrease water loss at high temperatures. These findings uncover the possibility for resurrection of a cuticular triterpenoid-synthesizing gene in maize that could create a more heat-tolerant water barrier on the plant’s leaf surfaces. They also provide a fundamental understanding of sorghum leaf waxes that will inform efforts to divert surface carbon to intracellular storage for bioenergy and bioproduct innovations.

Climate and land-use effects on hydrological and vegetation signals during the last three millennia in southwestern Morocco

<p>The southwest of Morocco is considered to be an area of refuge within the Mediterranean region, hosting the endemic tropical Argan tree. This region is presently subject to severe droughts, desertification, and land degradation, and likely facing increased climate variability and socio-economic stress in the future. Here, we use the stable hydrogen and carbon isotope composition (δD and δ13C) of plant-waxes in a high-resolution marine sediment core (GeoB8601-3) collected off Cape Ghir in southwestern Morocco, in combination with published data on pollen and XRF element ratios from the same archive. We aim to reconstruct the hydroclimate and vegetation history during the last 3000 years. Stable carbon isotope compositions of leaf waxes (δ13Cwax) show that natural vegetation in southwestern Morocco consists of C3 plants. Minor variations in δ13Cwax were positively correlated to changes in stable hydrogen isotope compositions of leaf waxes (δDwax) before 700 CE. Changes in rainfall amounts and water use efficiency indicate a clear vegetation response to precipitation changes and thus to climate forcing. After 700 CE, δDwax and δ13Cwax became de-coupled suggesting that the plant wax discharge and their isotope signals were no longer solely controlled by climate; the waxes likely mainly originate from the lowlands and carry an enriched (dry) δD signal but a depleted 13C signature. The depletion of δ13Cwax correlates with the increase of Argan pollen concentration in the record. The period between ~700 and 900 CE coincides with the Arabization of Morocco which had an impact on the demographic composition of the country leading to new agricultural habits and, as a result, on the land-use triggering a higher erosion of lowland material by the Souss River.</p>

Compound-specific radiocarbon ages of soil and sedimentary leaf wax biomarkers in an arid high-altitude environment

<p>Compound-specific radiocarbon analysis (CSRA) of leaf waxes has revealed significant lag times before compounds are deposited in marine and lacustrine sediments. No such data so far exist for a cold and arid high altitude lake system, where carbon turnover and biomarker fluxes to sediments are expected to be relatively low. To elucidate transport dynamics of terrestrial leaf waxes in such an environment (MAT: -4°C, MAP <100mm), we determined CSRA-ages of selected long-chain <em>n</em>-alkanes in surface soil samples (0-10 cm), collected from alpine meadows in the catchment of Lake Karakul (Pamirs, Tajikistan), and in two sections  of a well dated sediment core from the same lake. We aimed to answer the question if there is a potential bias in the interpretation of biomarker records, in case the leaf wax compounds are significantly older than the sediment age-model suggests.</p><p><em>n</em>C<sub>29</sub>- and <em>n</em>C<sub>31</sub>-alkanes in the soil samples exhibited variable ages, ranging from 105±79 to 2260±155 cal. yrs BP. In the two sediment core samples, three of the four obtained ages for <em>n</em>C<sub>29</sub> and <em>n</em>C<sub>31</sub> felt on the very lower ends of the 1ϭ-uncertainty ranges of modelled ages (based on AMS <sup>14</sup>C<sub>TOC</sub> and OSL dating results).</p><p>The large span of CSRA-ages of soils gives evidence for heterogeneous decomposition and transport conditions in the lake catchment. We hypothesize that compounds with longest pre-aging contributed in lower proportions to the accumulated lake sediments and further suggest that sedimentary leaf waxes represent compounds with intermediate turnover time in soils, for example originating from alluvial plains close to the shores. Overall, the obtained results give evidence that sedimentary leaf wax compounds in this cold and arid high altitude setting are potentially older than the conventional age-model indicates. On the other hand, these findings need to be interpreted in context of the generally large uncertainty ranges of such age-models, which are further influenced by unknown factors for example changes of reservoir effects. </p>

Climate and land-use effects on hydrological and vegetation signals during the last three millennia: Evidence from sedimentary leaf waxes in southwestern Morocco

The southwest of Morocco is considered to be an area of refuge within the Mediterranean region, hosting the endemic tropical Argan tree. This region is presently subject to severe droughts, desertification and land degradation, and likely facing increased climate variability and socio-economic stress in the future. Here, we use the stable hydrogen and carbon isotope composition (δD and δ13C) of plant-waxes in a high-resolution marine sediment core (GeoB8601-3) collected off Cape Ghir in southwestern Morocco, in combination with published data on pollen and XRF element ratios from the same archive. We aim to reconstruct the hydroclimate and vegetation history during the last 3000 years. Stable carbon isotope compositions of leaf waxes (δ13Cwax) show that natural vegetation in southwestern Morocco consists of C3 plants. Minor variations in δ13Cwax were positively correlated to changes in stable hydrogen isotope compositions of leaf waxes (δDwax) before 700 CE. Changes in rainfall amounts and water use efficiency indicate a clear vegetation response to precipitation changes and thus to climate forcing. After 700 CE, δDwax and δ13Cwax became de-coupled suggesting that the plant wax discharge and their isotope signals were no longer solely controlled by climate; the waxes likely mainly originate from the lowlands and carry an enriched (dry) δD signal but a depleted 13C signature. The depletion of δ13Cwax correlates with the increase of Argan pollen concentration in the record. The period between ~700 and 900 CE coincides with the Arabization of Morocco which had an impact on the demographic composition of the country leading to new agricultural habits and, as a result, on the land-use triggering a higher erosion of lowland material by the Souss River.