New Insights Into Lignification via Network and Multi-Omics Analyses of Arogenate Dehydratase Knock-Out Mutants in Arabidopsis thaliana

Multiple Arabidopsis arogenate dehydratase (ADT) knock-out (KO) mutants, with phenotypes having variable lignin levels (up to circa 70% reduction), were studied to investigate how differential reductions in ADTs perturb its overall plant systems biology. Integrated “omics” analyses (metabolome, transcriptome, and proteome) of wild type (WT), single and multiple ADT KO lines were conducted. Transcriptome and proteome data were collapsed into gene ortholog (GO) data, with this allowing for enzymatic reaction and metabolome cross-comparisons to uncover dominant or likely metabolic biosynthesis reactions affected. Network analysis of enzymes–highly correlated to stem lignin levels–deduced the involvement of novel putative lignin related proteins or processes. These included those associated with ribosomes, the spliceosome, mRNA transport, aminoacyl tRNA biosynthesis, and phosphorylation. While prior work helped explain lignin biosynthesis regulation at the transcriptional level, our data here provide support for a new hypothesis that there are additional post-transcriptional and translational level processes that need to be considered. These findings are anticipated to lead to development of more accurate depictions of lignin/phenylpropanoid biosynthesis models in situ, with new protein targets identified for further biochemical analysis and/or plant bioengineering. Additionally, using KEGG defined functional categorization of proteomics and transcriptomics analyses, we detected significant changes to glucosinolate, α-linolenic acid, nitrogen, carotenoid, aromatic amino acid, phenylpropanoid, and photosynthesis-related metabolic pathways in ADT KO mutants. Metabolomics results also revealed that putative carotenoid and galactolipid levels were generally increased in amount, whereas many glucosinolates and phenylpropanoids (including flavonoids and lignans) were decreased in the KO mutants.

Mass spectrometry based untargeted metabolomics for plant systems biology

Untargeted metabolomics enables the identification of key changes to standard pathways, but also aids in revealing other important and possibly novel metabolites or pathways for further analysis. Much progress has been made in this field over the past decade and yet plant metabolomics seems to still be an emerging approach because of the high complexity of plant metabolites and the number one challenge of untargeted metabolomics, metabolite identification. This final and critical stage remains the focus of current research. The intention of this review is to give a brief current state of LC–MS based untargeted metabolomics approaches for plant specific samples and to review the emerging solutions in mass spectrometer hardware and computational tools that can help predict a compound's molecular structure to improve the identification rate.

Early Career Researcher Profile: Honglong Zhao

Honglong Zhao is a postdoctoral researcher at the Chinese Academy of Sciences Center for Excellence in Molecular Plant Sciences. There, he studies plant systems biology and works on the mathematical model construction of primary metabolism in C3 photosynthetic leaves. He received a PhD in Botany in 2020 working with Prof. Xinguang Zhu.

The grant is dead, long live the data - migration as a pragmatic exit strategy for research data preservation

Open research, data sharing and data re-use have become a priority for publicly- and charity-funded research. Efficient data management naturally requires computational resources that assist in data description, preservation and discovery. While it is possible to fund development of data management systems, currently it is more difficult to sustain data resources beyond the original grants. That puts the safety of the data at risk and undermines the very purpose of data gathering. PlaSMo stands for ‘Plant Systems-biology Modelling’ and the PlaSMo model repository was envisioned by the plant systems biology community in 2005 with the initial funding lasting until 2010. We addressed the sustainability of the PlaSMo repository and assured preservation of these data by implementing an exit strategy. For our exit strategy we migrated data to an alternative, public repository with secured funding. We describe details of our decision process and aspects of the implementation. Our experience may serve as an example for other projects in a similar situation. We share our reflections on the sustainability of biological data management and the future outcomes of its funding. We expect it to be a useful input for funding bodies.

The grant is dead, long live the data - migration as a pragmatic exit strategy for research data preservation

Open research, data sharing and data re-use have become a priority for publicly- and charity-funded research. Efficient data management naturally requires computational resources that assist in data description, preservation and discovery. While it is possible to fund development of data management systems, currently it is more difficult to sustain data resources beyond the original grants. That puts the safety of the data at risk and undermines the very purpose of data gathering. PlaSMo stands for ‘Plant Systems-biology Modelling’ and the PlaSMo model repository was envisioned by the plant systems biology community in 2005 with the initial funding lasting till 2010. We addressed the sustainability of the PlaSMo repository and assured preservation of these data by implementing an exit strategy. For our exit strategy we migrated data to an alternative public repository of secured funding. We describe details of our decision process and aspects of the implementation. Our experience may serve as an example for other projects in similar situation. We share our reflections on sustainability of biological data management and the future outcomes of its funding. We expect it to be a useful input for funding bodies.