Abstract
The study of loading rate of microorganisms in the space of hospital inside and outside to evaluate their impacts on physical health of patients and staff, as well as to find out the source of possible infections and allergies stemming from the presence of bioaerosols is of great importance. In total, 262 bacterial and fungal samples were collected from the air of the wards of Tohid Hospital, Sanandaj, Iran. Also, suspended particles were measured by Particle Mass Counter (model: TES-5200). Parameter such as relative humidity and temperature were recorded by using TES-5200 device. To identify bacteria, some biochemical and molecular tests were conducted. And, for the identification of fungi, microscopic and macroscopic characteristics were used. The highest and lowest densities of the bioaerosols were observed in lung and operating wards (336.67 and 15.25 CFU/m3). Moreover, the highest and least concentrations of particles were seen in the emergency and operating wards, respectively. The most common fungi isolated from the hospital air were Penicillium (24.7%), Cladosporium (23. 4%), Aspergillus niger (13.3%) and Aspergillus Flavus (11.4%). Furthermore, the highest concentration of the isolated bacterium was Staphylococcus hemolyticus (31.84%). And, the bacteria had the most resistance to antibiotic gentamicin.The general average of air pollution of the hospital to bioaerosols in quantitative terms was higher than that suggested by international organizations. Considering the high concentration of bioaerosols and particles in the hospital studied, provision of optimal conditions (like temperature, humidity, suitable ventilation and intelligent air conditioning system) and imposing a restriction in the entrance of the wards can be utilized to reduce the amount of pollution.