GSM Base Automated CPR Device Using Scotch-Yoke Mechanism

Cardiopulmonary resuscitation (CPR) is another lifesaving technique which will increase the chances of survival after getting cardiac arrest but when done manually it comes with Many problems like knowledge about CPR technique amongst the general population and inability of human to provide CPR as per WHO guideline and as long it needed. The main objective is to make automated CPR device which will provide strokes on chest through motor best scotch yoke mechanism and will give artificial ventilation through resuscitation bag true motor base side crank mechanism with the pulse sensor which will monitor the pulse and give signal to start and stop CPR device. GSM module which will send emergency message to the near hospital after confirming cardiac arrest.

Role of pKA in Charge Regulation and Conformation of Various Peptide Sequences

Peptides containing amino acids with ionisable side chains represent a typical example of weak ampholytes, that is, molecules with multiple titratable acid and base groups, which generally exhibit charge regulating properties upon changes in pH. Charged groups on an ampholyte interact electrostatically with each other, and their interaction is coupled to conformation of the (macro)molecule, resulting in a complex feedback loop. Their charge-regulating properties are primarily determined by the pKA of individual ionisable side-chains, modulated by electrostatic interactions between the charged groups. The latter is determined by the amino acid sequence in the peptide chain. In our previous work we introduced a simple coarse-grained model of a flexible peptide. We validated it against experiments, demonstrating its ability to quantitatively predict charge on various peptides in a broad range of pH. In the current work, we investigated two types of peptide sequences: diblock and alternating, each of them consisting of an equal number of amino acids with acid and base side-chains. We showed that changing the sequence while keeping the same overall composition has a profound effect on the conformation, whereas it practically does not affect total charge on the peptide. Nevertheless, the sequence significantly affects the charge state of individual groups, showing that the zero net effect on the total charge is a consequence of unexpected cancellation of effects. Furthermore, we investigated how the difference between the pKA of acid and base side chains affects the charge and conformation of the peptide, showing that it is possible to tune the charge-regulating properties by following simple guiding principles based on the pKA and on the amino acid sequence. Our current results provide a theoretical basis for understanding of the complex coupling between the ionisation and conformation in flexible polyampholytes, including synthetic polymers, biomimetic materials and biological molecules, such as intrinsically disordered proteins, whose function can be regulated by changes in the pH.

Quantitative Prediction of Charge Regulation in Oligopeptides

Weak ampholytes are ubiquitous in nature and commonly found in artificial pH-responsive systems. However, our limited understanding of their charge regulation and the lack of predictive capabilities hinder the bottom-up design of such systems. Here, we used a coarse-grained model of a flexible polymer with weakly ionisable monomer units to quantitatively analyse the ionisation behaviour of two oligopeptides. Our model predicts differences in the charge states between oligopeptides and monomeric amino acids, showing that conformational flexibility and electrostatic interactions between weak acid and base side chains play a key role in the charge regulation. By comparing our simulations with experimental results from potentiometric titration, capillary zone electrophoresis and NMR, we demonstrated that our model reliably predicts the charge state of various peptide sequences. Ultimately, our model is the first step towards understanding the charge regualtion in flexible disordered proteins, and towards using predictive bottom-up design of responsive ampholytes to tailor their<br>properties as a function of charge and pH.<br>

Quantitative Prediction of Charge Regulation in Oligopeptides

Weak ampholytes are ubiquitous in nature and commonly found in artificial pH-responsive systems. However, our limited understanding of their charge regulation and the lack of predictive capabilities hinder the bottom-up design of such systems. Here, we used a coarse-grained model of a flexible polymer with weakly ionisable monomer units to quantitatively analyse the ionisation behaviour of two oligopeptides. Our model predicts differences in the charge states between oligopeptides and monomeric amino acids, showing that conformational flexibility and electrostatic interactions between weak acid and base side chains play a key role in the charge regulation. By comparing our simulations with experimental results from potentiometric titration, capillary zone electrophoresis and NMR, we demonstrated that our model reliably predicts the charge state of various peptide sequences. Ultimately, our model is the first step towards understanding the charge regualtion in flexible disordered proteins, and towards using predictive bottom-up design of responsive ampholytes to tailor their<br>properties as a function of charge and pH.<br>

Quantitative Prediction of Charge Regulation in Oligopeptides

Weak ampholytes are ubiquitous in nature and commonly found in artificial pH-responsive systems. However, our limited understanding of their charge regulation and the lack of predictive capabilities hinder the bottom-up design of such systems. Here, we used a coarse-grained model of a flexible polymer with weakly ionisable monomer units to quantitatively analyse the ionisation behaviour of two oligopeptides. Our model predicts differences in the charge states between oligopeptides and monomeric amino acids, showing that conformational flexibility and electrostatic interactions between weak acid and base side chains play a key role in the charge regulation. By comparing our simulations with experimental results from potentiometric titration, capillary zone electrophoresis and NMR, we demonstrated that our model reliably predicts the charge state of various peptide sequences. Ultimately, our model is the first step towards understanding the charge regualtion in flexible disordered proteins, and towards using predictive bottom-up design of responsive ampholytes to tailor their<br>properties as a function of charge and pH.<br>

Quantitative Prediction of pH-Controlled Ionization of Oligoampholytes

Weak ampholytes are ubiquitous in nature and commonly found in artificial pH-responsive systems. However, our limited understanding of their ionisation response and the lack of predictive capabilities hinder the bottom-up design of such systems. Here, we used a coarse-grained model of a flexible polymer with weakly ionisable monomer units to quantitatively analyse the ionisation behaviour of two oligopeptides. Differences in ionisation response between oligopeptides and monomeric amino acids showed that electrostatic interactions between weak acid and base side chains play a key role in oligopeptide ionisation, as predicted by our model. Moreover, by comparing our simulations with experimental results from potentiometric titration, capillary zone electrophoresis and NMR, we demonstrated that our model reliably predicts the ionisation response and electrophoretic mobilities of various peptide sequences. Ultimately, our model is the first step towards using predictive bottom-up design of responsive ampholytes to tailor their properties as a function of charge and pH.<br>

Quantitative Prediction of pH-Controlled Ionization of Oligopeptides

Weak ampholytes are ubiquitous in nature and commonly found in artificial pH-responsive systems. However, our limited understanding of their ionisation response and the lack of predictive capabilities hinder the bottom-up design of such systems. Here, we used a coarse-grained model of a flexible polymer with weakly ionisable monomer units to quantitatively analyse the ionisation behaviour of two oligopeptides. Differences in ionisation response between oligopeptides and monomeric amino acids showed that electrostatic interactions between weak acid and base side chains play a key role in oligopeptide ionisation, as predicted by our model. Moreover, by comparing our simulations with experimental results from potentiometric titration, capillary zone electrophoresis and NMR, we demonstrated that our model reliably predicts the ionisation response and electrophoretic mobilities of various peptide sequences. Ultimately, our model is the first step towards using predictive bottom-up design of responsive ampholytes to tailor their properties as a function of charge and pH.<br>

Numerical Analysis of the Effect of Offshore Turbulent Wind Inflow on the Response of a Spar Wind Turbine

Turbulent wind at offshore sites is known as the main cause for fatigue on offshore wind turbine components. Numerical simulations are commonly used to predict the loads and motions of floating offshore wind turbines; however, the definition of representative wind input conditions is necessary. In this study, the load and motion responses of a spar-type Offshore Code Comparison Collaboration (OC3) wind turbine under different turbulent wind conditions is studied and investigated by using SIMO-Riflex in Simulation Workbench for Marine Applications (SIMA) workbench. Using the two spectral models given in the International Electrotechnical Commission (IEC) standards, it is found that a lower wind lateral coherence under neutral atmospheric stability conditions results in an up to 27% higher tower base side–side bending moment and a 20% higher tower top torsional moment. Comparing different atmospheric stability conditions simulated using a spectral model based on FINO1 wind data measurement, the highest turbulent energy content under very unstable conditions yields a 26% higher tower base side–side bending moment and a 27% higher tower top torsional moment than neutral conditions, which have the lowest turbulent energy content and turbulent intensity. The yaw-mode of the OC3 wind turbine is found to be the most influenced component by assessing variations in both the lateral coherence and the atmospheric stability conditions.

A Novel Planar Monopole Antenna with Truncated Ground Plane for Wireless Communication

In this paper, triple-band planar monopole Microstrip Antenna intended for different applications like Bluetooth, Wi-Fi, Wireless LAN (2.4 GHz), LTE 2500 band, WiMax (3.5 GHz), and a piece of C-band applications. The reception apparatus has been intended to work at different recurrence groups, for example, 2.25 – 2.5 GHz, 3.32 – 3.97 GHz, and 5.90 – 8.67 GHz individually. The proposed receiving antenna comprises of a planar monopole reception apparatus imprinted on RT/duroid 5880 substrate (through a general permittivity of 2.2 and loss tangent of 0.0009) and the base side printed with a truncated ground. The planar monopole reception apparatus has been viably structured and reproduced by utilizing Ansys-HFSS design tool. The component of the proposed receiving antenna is 40 × 28.4 × 1.575 mm3 . The reenacted outcome shows return loss, voltage standing wave ratio (VSWR), radiation pattern, and gain of the Antenna. Also, the truncated ground plane structure is straightforward, vigorous and possesses little space, building it appropriate for different applications.