Metabarcoding Analyses of Gut Microbiota of Nile Tilapia (Oreochromis niloticus) from Lake Awassa and Lake Chamo, Ethiopia

The Nile tilapia (Oreochromis niloticus) gut harbors a diverse microbial community; however, their variation across gut regions, lumen and mucosa is not fully elucidated. In this study, gut microbiota of all samples across gut regions and sample types (luminal content and mucosa) were analyzed and compared from two Ethiopian lakes. Microbiota were characterized using 16S rRNA Illumina MiSeq platform sequencing. A total of 2061 operational taxonomic units (OTUs) were obtained and the results indicated that Nile tilapia from Lake Chamo harbored a much more diversified gut microbiota than Lake Awassa. In addition, the gut microbiota diversity varied significantly across the gut region based on the Chao1, Shannon and Simpson index. The microbiome analyses of all samples in the midgut region showed significantly higher values for alpha diversity (Chao 1, Shannon and Simpson). Beta diversity analysis revealed a clear separation of samples according to sampling areas and gut regions. The most abundant genera were Clostridium_sensu_stricto and Clostridium_XI genera across all samples. Between the two sampling lakes, two phyla, Phylum Fusobacteria and Cyanobacteria, were found to be significantly different. On the other hand, six phyla (Actinobacteria, Bacteroidetes, Chloroflexi, Firmicutes, Proteobacteria and Cyanobacteria) were significantly different across gut regions. In this study, we found that all samples shared a large core microbiota, comprising a relatively large number of OTUs, which was dominated by Proteobacteria, Firmicutes, Cyanobacteria, Fusobacteria and Actinobacteria. This study has established the bases for future large-scale investigations of gut microbiota of fishes in Ethiopian lakes.

Diaphanosoma Fischer, 1850 (Crustacea: Cladocera: Sididae) of Lake Turkana (East Africa), with the description of a new species of the genus

Diaphanosoma turkanae sp. nov. is described from Lake Turkana (Kenya, East Africa). This species is the second Afrotropical endemic in the order Ctenopoda. It is characterized by a distinctive feature, the presence of an unusually small number of setae on the upper two-segmented antennal branch (exopodite); the proximal segment of the branch always bears three setae while the distal segment bears either six or seven setae. Morphologically the new species is most similar to D. orghidani Negrea, also occurring in Africa and presumably sharing an evolutionary affinity. D. turkanae sp. nov. co-occurs with two other species of the genus, D. lacustris Kořinek and D. excisum Sars, which are briefly described here. Temperate-subtropical species of the genus Diaphanosoma penetrate far south along the Nile River system up to the equatorial and Ethiopian lakes and further southwards, often forming multi-species assemblages.

The composition of fish communities of nine Ethiopian lakes along a north-south gradient: threats and possible solutions

Fish populations of nine Ethiopian freshwater lakes were quantitatively sampled with a standardized protocol, using multi-mesh gill nets. In total, 27 species were identified, but only 14 species were common. Based on the common species, the fish communities showed large differences in their species composition, except for Lake Abaya and Lake Chamo which were similar. Most fish species were observed in only one or two lakes. Compared with the information reported in literature the present study generally underestimated the species richness. The empirical model of Amarasinghe and Welcomme (2002) for African lakes was used to estimate fish species richness, which was compared with species presence reported in literature. Biodiversity in the two northern highland lakes is low, but not lower than the model estimate. Lake Tana has a high biodiversity which is close to what is estimated by the model, but three Rift Valley lakes have low biodiversity, lower than estimated by the model. There are also strong indications for the Rift Valley lakes that species richness was higher in the past because the species richness reported in the older literature was generally much higher than those observed by us in the present study and those reported in the more recent literature. Threats like overfishing, high sediment load and degradation of habitats were identified. It is recommended that Ethiopia should develop guidelines for fishery legislation and implement it through an enforcement agency. Moreover, catchments management should be practiced to save the water bodies and their fish communities.