Transcriptome profiling of developing leaf and shoot apices to reveal the molecular mechanism and co-expression genes responsible for the wheat heading date

Background Wheat is one of the most widely planted crops worldwide. The heading date is important for wheat environmental adaptability, as it not only controls flowering time but also determines the yield component in terms of grain number per spike. Results In this research, homozygous genotypes with early and late heading dates derived from backcrossed progeny were selected to conduct RNA-Seq analysis at the double ridge stage (W2.0) and androgynous primordium differentiation stage (W3.5) of the leaf and apical meristem, respectively. In total, 18,352 differentially expressed genes (DEGs) were identified, many of which are strongly associated with wheat heading date genes. Gene Ontology (GO) enrichment analysis revealed that carbohydrate metabolism, trehalose metabolic process, photosynthesis, and light reaction are closely related to the flowering time regulation pathway. Based on MapMan metabolic analysis, the DEGs are mainly involved in the light reaction, hormone signaling, lipid metabolism, secondary metabolism, and nucleotide synthesis. In addition, 1,225 DEGs were annotated to 45 transcription factor gene families, including LFY, SBP, and MADS-box transcription factors closely related to flowering time. Weighted gene co-expression network analysis (WGCNA) showed that 16, 336, 446, and 124 DEGs have biological connections with Vrn1-5 A, Vrn3-7B, Ppd-1D, and WSOC1, respectively. Furthermore, TraesCS2D02G181400 encodes a MADS-MIKC transcription factor and is co-expressed with Vrn1-5 A, which indicates that this gene may be related to flowering time. Conclusions RNA-Seq analysis provided transcriptome data for the wheat heading date at key flower development stages of double ridge (W2.0) and androgynous primordium differentiation (W3.5). Based on the DEGs identified, co-expression networks of key flowering time genes in Vrn1-5 A, Vrn3-7B, WSOC1, and Ppd-1D were established. Moreover, we discovered a potential candidate flowering time gene, TraesCS2D02G181400. Taken together, these results serve as a foundation for further study on the regulatory mechanism of the wheat heading date.

Model Simulation of M2 Internal Tide at the Mariana Double Ridges

In this study, M2 tidal energy and tide-induced mixing in the Mariana double ridges are investigated with a high-resolution three-dimensional non-hydrostatic numerical model and baroclinic energy budget analysis. The interference effect of the double ridges on the internal tide in the Mariana is examined by omitting either the eastern or the western ridge. Our results show that the baroclinic velocity on the sides of the interior facing slopes of the double ridges is larger than that on the other sides. In the double ridges, high values of dissipation reaching O (10−6 W kg−1) are accompanied by diapycnal diffusivity reaching O (10−1 m2 s−1), which is several orders of magnitude higher than the mixing of the open ocean. The bottom diapycnal mixing in the inner region between the two ridges is one order of magnitude larger than the mixing outside the ridges, indicating the important role of the interference of the double-ridge topography on the mixing in the Mariana Arc. Omitting either the eastern or the western ridge would have a significant impact on tide current, baroclinic energy flux and dissipation, and diapycnal mixing. The internal tide conversion, dissipation, and flux divergence are amplified by the double ridge topography, especially in the central part of the double ridges. Through energy budgets analysis, we conclude that the eastern ridge is the main source of the baroclinic tide in the Mariana double ridges.

Observations of Stably Stratified Flow through a Microscale Gap

This paper reports the findings of a comprehensive field investigation on flow through a mountain gap subject to a range of stably stratified environmental conditions. This study was embedded within the Perdigão field campaign, which was conducted in a region of parallel double-ridge topography with ridge-normal wind climatology. One of the ridges has a well-defined gap (col) at the top, and an array of in situ and remote sensors, including a novel triple Doppler lidar system, was deployed around it. The experimental design was mostly guided by previous numerical and theoretical studies conducted with an idealized configuration where a flow (with characteristic velocity U0 and buoyancy frequency N) approaches normal to a mountain of height h with a gap at its crest, for which the governing parameters are the dimensionless mountain height G = Nh/U0 and various gap aspect ratios. Modified forms of G were proposed to account for real-world atmospheric variability, and the results are discussed in terms of a gap-averaged value Gc. The nature of gap flow was highly dependent on Gc, wherein a nearly neutral flow regime (Gc < 1), a transitional mountain wave regime [Gc ~ O(1)], and a gap-jetting regime [Gc > O(1)] were identified. The measurements were in broad agreement with previous numerical and theoretical studies on a single ridge with a gap or double-ridge topography, although details vary. This is the first-ever detailed field study reported on microscale [O(100) m] gap flows, and it provides useful data and insights for future theoretical and numerical studies.