Stiffness Variation of 3D Collagen Networks by Surface Functionalization of Network Fibrils with Sulfonated Polymers

Fibrillar collagen is the most prominent protein in the mammalian extracellular matrix. Therefore, it is also widely used for cell culture research and clinical therapy as a biomimetic 3D scaffold. Charged biopolymers, such as sulfated glycosaminoglycans, occur in vivo in close contact with collagen fibrils, affecting many functional properties such as mechanics and binding of growth factors. For in vitro application, the functions of sulfated biopolymer decorations of fibrillar collagen materials are hardly understood. Herein, we report new results on the stiffness dependence of 3D collagen I networks by surface functionalization of the network fibrils with synthetic sulfonated polymers, namely, poly(styrene sulfonate) (PSS) and poly(vinyl sulfonate) (PVS). A non-monotonic stiffness dependence on the amount of adsorbed polymer was found for both polymers. The stiffness dependence correlated to a transition from mono- to multilayer adsorption of sulfonated polymers on the fibrils, which was most prominent for PVS. PVS mono- and multilayers caused a network stiffness change by a factor of 0.3 and 2, respectively. A charge-dependent weakening of intrafibrillar salt bridges by the adsorbed sulfonated polymers leading to fibrillar softening is discussed as the mechanism for the stiffness decrease in the monolayer regime. In contrast, multilayer adsorption can be assumed to induce interfibrillar bridging and an increase in network stiffness. Our in vitro results have a strong implication on in vivo characteristics of fibrillar collagen I, as sulfated glycosaminoglycans frequently attach to collagen fibrils in various tissues, calling for an up to now overlooked impact on matrix and tendon mechanics.

Two 6/10-connected Cu12S6 cluster-based organic frameworks: crystal structure and proton conduction

Nowadays, although the exploration of proton conductive materials has been ranged from traditional sulfonated polymers to novel crystalline solid materials such as MOFs, COFs, and HOFs, the research on crystalline...

Interaction of synthetic and lignin-based sulfonated polymers with hydrophilic, hydrophobic, and charged self-assembled monolayers

The interaction mechanism of synthetic and lignin based sulfonated materials with well-designed functional surfaces was investigated systematically.

Synthesis and investigation of sulfonated poly(p-phenylene)-based ionomers with precisely controlled ion exchange capacity for use as polymer electrolyte membranes

Poly(p-phenylene)-based sulfonated polymers with well-controlled IECs were synthesized via a three-step procedure including preceding sulfonation of precursor monomers.

Preparation of Porous Polymeric Membranes Based on a Pyridine Containing Aromatic Polyether Sulfone

Polymeric membranes, based on a polysulfone-type aromatic polyether matrix, were successfully developed via the non-solvent induced phase separation (NIPS) method. The polyethersulfone type polymer poly[2-(4-(diphenylsulfonyl)-phenoxy)-6-(4-phenoxy) pyridine] (PDSPP) was used as the membrane matrix, and mixed with its sulfonated derivative (SPDSPP) and a polymeric porogen. The SPDPPP was added to impart hydrophilicity, while at the same time maintaining the interactions with the non-sulfonated aromatic polyether forming the membrane matrix. Different techniques were used for the membranes’ properties characterization. The results revealed that the use of the non-sulfonated and sulfonated polymers of the same polymeric backbone, at certain compositions, can lead to membranes with controllable porosity and hydrophilicity.