scholarly journals The appearance of DNA bands pattern based on the result of primary selection of RAPD orchid Phaius spp.

2021 ◽  
Vol 905 (1) ◽  
pp. 012147
Author(s):  
S Hartati ◽  
Samanhudi ◽  
O Cahyono
Keyword(s):  
Molecular Analysis ◽  
Plant Conservation ◽  
Pcr Amplification ◽  
Dna Amplification ◽  
Band Pattern ◽  
Botanical Gardens ◽  
Research Material ◽  
Clear Band ◽  
Primary Selection ◽  
Selection Of

Abstract This study aimed to examine the appearance of the DNA band pattern resulted from the selection of RAPD primers on Phaius spp. namely Phaius tankervillae, Phaius montanus, Phaius collasus and Phaius amboinensis. The research material was performed in the Center for Plant Conservation of the LIPI Bogor Botanical Gardens. Molecular analysis was carried out at the Laboratory of the Center for Horticultural and Tropical Studies IPB using RAPD. The study showed that the 2 primers RAPD OPA 02 and OPA 16 can be used for DNA amplification of orchids Phaius spp (Phaius tankervillae, Phaius montanus Phaius collasus and Phaius amboinensis) because they produce clear DNA bands. The result of PCR amplification on Phaius tankervillae, Phaius montanus, Phaius collasus, and Phaius amboinensis using OPA 02 and OPA 16 primers produced 11 and 9 DNA bands, respectively, with an average of 5 DNA bands per primer. In the band pattern at 800 bp on OPA 02 primers resulting sharp and clear band pattern quality.

MANIFESTATION OF TRANSGRESSION BY MAIN QUANTITATIVE CHARACTERISTICS OF WINTER TRITICAL PRODUCTIVITY IN F2 HYBRID POPULATIONS

2021 ◽  
Vol 1 ◽  
pp. 3-7
Author(s):  
В. Д. Тромсюк ◽  
◽  
В. Д. Бугайов ◽  
Keyword(s):  
Maximum Degree ◽  
Geographical Origin ◽  
Selection Process ◽  
Diallel Analysis ◽  
Grain Weight ◽  
Maximum Frequency ◽  
Winter Triticale ◽  
Research Material ◽  
Quantitative Characteristics ◽  
Selection Of

The research was conducted in 2014-2017 in the department of selection of fodder, grain ears, and industrial crops in the fields of scientific crop rotation of the Institute of Fodder and Agriculture of Podillya NAAS of Ukraine. A collection of 114 hexaploid specimens of different ecological and geographical origins was used as research material. Six varieties of winter triticale of different ecological and geographical origin and the manifestation of valuable economic features were used in crosses according to the scheme of full diallel analysis: Polovetske (UA0602494), Amos (UA0602627) originating from Ukraine; Kapryz (UA0601781), Tsekad 90 (UA0602066) - Russia; Dubrava (UA0602222) - Belarus and Pawo (UA0602555) - Poland. Only the best plants, which did not lag in growth and were normally developed, were selected for hybridization. The obtained generation F0 was sown in 2016, and generation F1 – 2017 manually in an experimental field with the same depth, which provides plants with the same area of nutrition. After full maturation, the plants were also collected manually in sheaves, structural analysis was performed on the main indicators of productivity. The frequency and degree of manifestation of positive transgressions in hybrids of the second generation (F2) of winter triticale were evaluated based on productivity: productive bushiness, number and weight of ear grains and grain weight from the plant. The best hybrids by degree and frequency of manifestation of positive transgression are selected. The degree of transgressions in productive bushiness averaged 65.3%, frequency - 43%; number of grains in the main ear - 7.4%, frequency - 15.3%; weight of grain in the ear - 13.8% at a frequency of 27, 7%; weight of grain from the plant - 91.5% with a transgression frequency of 52.7%. The best hybrid combinations of winter triticale on the basis of "productive bushiness" - Polovtsian / Caprice and Dubrava / Polovtsian (Tc = 100%), the maximum frequency of transgressions on this basis - hybrids Polovtsian / Caprice / Pawos and Pawo are selected and offered for use in the selection process. TC = 60); "Number of grains from the ear" - the maximum degree of transgressions was found in the hybrid Pawo / Polovtsian (Tc = 18.1%), the maximum frequency - in the hybrid Caprich / Pawo (Tch = 40); "Ear grain mass" - Pawo / Amos (Tc = 37.0%), the highest frequency of transgressions - in hybrids Amos / Dubrava and Kaprikh / Pawo (Tch = 50); "Grain weight from the plant" - Polovtsian / Amos (Tc = 37.0%), the frequency of transgressions - hybrids Polovtsian / Amos and Polovtsian / Caprice (Tc = 85%).

scholarly journals KENYAMANAN IKLIM LOKASI WISATA BERBASIS ALAM DI KAWASAN TROPIS

Agromet ◽  
2019 ◽  
Vol 33 (2) ◽  
pp. 53-61
Author(s):  
Nofi Yendri Sudiar
Keyword(s):  
Weather Conditions ◽  
Education Level ◽  
Tourism Industry ◽  
Botanical Gardens ◽  
Visitor Perceptions ◽  
Survey Results ◽  
Climate Comfort ◽  
Selection Of

This research reveals visitors perceptions of climate comfort in nature-based tourism areas in Ecopark Ancol, Bogor Botanical Gardens (KRB) and Cibodas Botanical Gardens (KRC). In addition to calculating the comfort score using the TCI and HCI methods and modifying their thermal aspects, a survey was also carried out in all three tourism areas simultaneously. The survey was conducted to collect data on climate comfort perceptions and the role of the weather on these comfort. A total of 793 respondents participated in this study. The majority of visitors stated that the weather affected the comfort of the climate during the tour. But weather conditions do not fully influence decisions in the selection of tourist visits. The level of perceived climate comfort for the three tourism sites namely Ecopark was perceived as neutral (57.3%), KRB was perceived as comfortable (60%) and KRC was perceived as comfortable (78.4%). While based on the score calculation approaching the survey results in Ecopark is TCI index modified in its thermal aspect with PET Tianjin (57.2). KRB is HCI without modification (59) and KRC is HCI modified by its thermal aspect with PET Tianjin (77.6). Statistically there are significant differences between sex, age, education level and topography. By understanding visitor perceptions, strategies and appropriate actions can be developed to increase comfort in the nature-based tourism industry.

scholarly journals Primary selection of a pedicled rectus femoris flap for protection of aortic graft

2019 ◽  
Vol 5 (4) ◽  
pp. 512-517
Author(s):  
Carissa L. Patete ◽  
Kriya Gishen ◽  
Ajani Nugent ◽  
Jorge Rey
Keyword(s):  
Rectus Femoris ◽  
Aortic Graft ◽  
Primary Selection ◽  
Selection Of

scholarly journals Selectivity of herbicides in the post-emergence sugarcane seedlings in primary selection fields

2019 ◽  
pp. 1232-1235
Author(s):  
Tácio Peres Silva ◽  
Amanda Mendes de Moura ◽  
Bruno Manoel Rezende de Melo ◽  
Douglas Goulart Castro ◽  
Laís Moretti Tomé ◽  
...  
Keyword(s):  
Sandy Soils ◽  
Mass Selection ◽  
Selective Action ◽  
Herbicide Treatments ◽  
Primary Selection ◽  
Establishment Phase ◽  
Final Selection ◽  
Selection Of

The hypothesis here proposed is that, in primary fields of mass selection (F1) of sugarcane, herbicides exert a selective action when applied in the early post-emergence of seedlings (POST-i) and supplemented after their establishment phase (POST-l) even in sandy soils. To verify such hypothesis, the selectivity of herbicides was studied on seedlings of three sugarcane families, after their application both at early and late post-emergence in primary selection fields (F1). The field had a randomized blocks design, with five replications, and the subdivided plots scheme was used. Twelve herbicide treatments were allocated in the plots (27 m-2), whereas the sub-plots (9 m-2) contained three families of seedlings. Each sub-plot consisted of a transplanted row (0.5 x 1.50 m) with 12 individuals of the same family. Six treatments were employed, namely, T1: tebuthiuron (1200 g ha-1) post-i + ametryn (3000 g ha-1) post-l; T2: diuron (1404 g ha-1) + hexazinone (396 g ha-1) post-i + ametryn (3000 g ha-1) post-l; T3: sulfentrazone (800 g ha-1) post-i + ametryn (3000 g ha-1) post-l; T4: diuron (1404 g ha-1) + hexazinone (396 g ha-1) post-i + metribuzin (1920 g ha-1) post-l; T5: sulfentrazone (800 g ha-1) post-i + metribuzin (1920 g ha-1) post-l, and T6: weeded plot. The sugarcane families were: F400 (♀IAC086155 x ♂unknown), F43 (♀IACBIO264 × ♂IAC911099), and F14 (♀IACSP991305 × ♂GlagaH). We concluded that the herbicides tebuthiuron (1200 g ha-1), diuron (104 g ha-1) + hexazinone (396 g ha-1), or sulfentrazone (800 g ha-1), when applied in the early post-emergence and supplemented with ametryn (3000 g ha-1) or metribuzin (1920 g ha-1) in late post-emergence (60 days from planting) of the sugarcane families studied, were selective to the seedlings. That is, no toxicity symptoms with a potential to impede the final selection of seedlings occurred.

scholarly journals Education and training in Brazilian botanical gardens: are we achieving GSPC targets?

Rodriguésia ◽  
2018 ◽  
Vol 69 (4) ◽  
pp. 1603-1612
Author(s):  
Tania Maria Cerati
Keyword(s):  
Plant Diversity ◽  
Public Awareness ◽  
Plant Conservation ◽  
Global Strategy ◽  
Ex Situ ◽  
The Public ◽  
Botanical Gardens ◽  
Representative Species ◽  
Education And Awareness

Abstract Engage, educate and raise public awareness of the importance of plant diversity is the means through which plant conservation can be achieved. The Global Strategy for Plant Conservation (GSPC) is a programme to halt the loss of plant diversity and recognize botanical gardens as a partners. This paper investigates the implementation of targets 14 and 15 GSPC in Brazilian botanic gardens specially in education and awareness about plant diversity and trained people. A survey was carried out on the institutional websites of 21 gardens registered in the National System of Botanical Gardens Registration to identify actions that follow the guidelines of these two targets. Websites outdated, difficult access and lack online resources for the public shows us that internet tools are poorly used to divulgation. The target 14's activities performed in most gardens are educational visits and environmental interpretation and explore collections in situ and ex situ from representative species of regional flora. Trained people is held in all gardens but some stand out as important research centers and graduate programs. Despite the efforts Brazilian botanical gardens need improve the public communication and develop strategies to measure the targets progress.

Phylogenetic grouping and identification of Rhizobium isolates on the basis of random amplified polymorphic DNA profiles

1993 ◽  
Vol 39 (7) ◽  
pp. 665-673 ◽  
Author(s):  
John J. Dooley ◽  
Stephen P. Harrison ◽  
Lance R. Mytton ◽  
Malcolm Dye ◽  
Ann Cresswell ◽  
...  
Keyword(s):  
Rhizobium Leguminosarum ◽  
Rhizobium Meliloti ◽  
Random Amplified Polymorphic Dna ◽  
Dna Amplification ◽  
Distinct Group ◽  
Principal Coordinate Analysis ◽  
The Other ◽  
Phylogenetic Grouping ◽  
Dna Profiles ◽  
Selection Of

Through the use of a single, random 15mer as a primer, between 1 and 12 DNA amplification products were obtained per strain from a selection of 84 Rhizobium and Bradyrhizobium isolates. A principal-coordinate analysis was used to analyse the resulting amplified DNA profiles and it was possible to assign isolates to specific groupings. Within the species Rhizobium leguminosarum, the biovar phaseoli formed a distinct group from the other biovars of the species, viciae and trifolii, which grouped together. Isolates of Rhizobium meliloti and Bradyrhizobium species formed their own clear, specific groups. Although it was possible to identify individual isolates on the basis of differences in their amplified DNA profiles, there was evidence that some amplified segments were conserved among individuals at the biovar and species levels.Key words: Rhizobium, DNA amplification, random primers.

Primary selection of viral inhibitors in tissue culture

1978 ◽  
Vol 12 (11) ◽  
pp. 1423-1427
Author(s):  
V. I. Votyakov ◽  
M. N. Shashikhina ◽  
S. V. Zhavrid ◽  
G. I. Zhungietu ◽  
M. A. Rekhter ◽  
...  
Keyword(s):  
Tissue Culture ◽  
Primary Selection ◽  
Selection Of ◽  
Viral Inhibitors

scholarly journals Evaluation of selective media for the isolation of Brachyspira aalborgi from human faeces

2003 ◽  
Vol 52 (6) ◽  
pp. 509-513 ◽  
Author(s):  
C. Josephine Brooke ◽  
Thomas V. Riley ◽  
David J. Hampson
Keyword(s):  
Pcr Amplification ◽  
Brain Heart Infusion ◽  
Polymyxin B ◽  
Isolation Medium ◽  
Human Faeces ◽  
Solid Media ◽  
Faecal Samples ◽  
The Media ◽  
Brachyspira Aalborgi ◽  
Selection Of

The purposes of this study were to identify a solid medium that supports improved growth of the anaerobic intestinal spirochaete Brachyspira aalborgi, to modify this for use as a selective isolation medium and then to test the medium for its effectiveness in isolating B. aalborgi from patients’ faeces. Of the media evaluated, brain heart infusion agar (BHIA) with 10 % bovine blood (BB) was the most effective base–supplement combination for growth, with colonies attaining 1.2 mm in diameter by 21 days. Incubation in an anaerobic jar (94 % H2, 6 % CO2) permitted growth of larger colonies than incubation in an anaerobic chamber (80 % N2, 10 % H2, 10 % CO2). Growth was improved only slightly at 38.5 °C compared with 37 °C. Selection of B. aalborgi from artificially seeded faeces was achieved equally well on eight different solid media containing spectinomycin (400 μg ml−1) alone or in combinations with polymyxin B (5 μg ml−1), colistin (25 μg ml−1) and rifampicin (12.5 μg ml−1). By using BHIA 10 % BB with spectinomycin plus polymyxin B, B. aalborgi was isolated from one of five human faecal samples that were positive for B. aalborgi by PCR amplification. This is the first report of the isolation of B. aalborgi from human faeces.

scholarly journals Cell surface lipid composition and hydrophobicity governs tuberculosis evolution and pathogenicity

2020 ◽  
Vol 2 (7A) ◽  
Author(s):  
David Minnikin
Keyword(s):  
Late Pleistocene ◽  
Lipid Composition ◽  
Homo Sapiens ◽  
Cell Envelope ◽  
Dna Amplification ◽  
Bone Lesions ◽  
Surface Lipid ◽  
Fertile Crescent ◽  
Phthiocerol Dimycocerosates ◽  
Selection Of

The evolution of tubercle bacilli correlates closely with changes in cell envelope surface lipid composition (Donoghue et al. Diversity 2017, 9:46; Jankute et al. Scientific Reports 2017, 7:1315). Smooth, hydrophilic “Mycobacterium canettii” is the first recognisable member of the Mycobacterium tuberculosis complex, but it has reduced pathogenicity and poor aerosol transmission. In contrast, rough M. tuberculosis is very hydrophobic and readily spread in aerosols. Starting from hydrophilic surface lipids in environmental Mycobacterium kansasii, intermediate “M. canettii” adds hydrophobic lipids but retains overall cell hydrophilicity. Eliminating hydrophilic lipooligosaccharides (LOSs) and phenolic glycolipids (PGLs) from “M. canettii” leads to M. tuberculosis with a refined selection of hydrophobic lipids, namely phthiocerol dimycocerosates (PDIMs), pentaacyl trehaloses (PATs) and sulfoglycolipids (SGLs). The relative hydrophobicity of M. tuberculosis is double that of representatives of M. kansasii and “M. canettii”. The above changes have implications both for the onset of tuberculosis and pinpointing evolutionary hosts. Tuberculosis has not been found in Homo sapiens during the Late Pleistocene, but megafauna are the most likely hosts; characteristic bone lesions have been validated by TB DNA amplification and lipid biomarkers in bison metacarpals up to 17,000 years old. Late Pleistocene enhanced TB hydrophobicity and aerosolisation may have produced megafaunal pandemics, with extinction of bison, mastodons and contemporary taxa. The oldest H. sapiens tuberculosis is from the “Fertile Crescent” back to 9-11ka BP at the start of the Holocene. Naïve humans arriving “Out of Africa” may have encountered newly virulent tubercle bacilli of megafaunal origin, recently refined through a distinct “bottleneck”.

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