Carbon Materials Advancing Microorganisms in Driving Soil Organic Carbon Regulation

Carbon emission from soil is not only one of the major sources of greenhouse gases but also threatens biological diversity, agricultural productivity, and food security. Regulation and control of the soil carbon pool are political practices in many countries around the globe. Carbon pool management in engineering sense is much bigger and beyond laws and monitoring, as it has to contain proactive elements to restore active carbon. Biogeochemistry teaches us that soil microorganisms are crucial to manage the carbon content effectively. Adding carbon materials to soil is thereby not directly sequestration, as interaction of appropriately designed materials with the soil microbiome can result in both: metabolization and thereby nonsustainable use of the added carbon, or—more favorably—a biological amplification of human efforts and sequestration of extra CO2 by microbial growth. We review here potential approaches to govern soil carbon, with a special focus set on the emerging practice of adding manufactured carbon materials to control soil carbon and its biological dynamics. Notably, research on so-called “biochar” is already relatively mature, while the role of artificial humic substance (A-HS) in microbial carbon sequestration is still in the developing stage. However, it is shown that the preparation and application of A-HS are large biological levers, as they directly interact with the environment and community building of the biological soil system. We believe that A-HS can play a central role in stabilizing carbon pools in soil.

Impacts of Rice Field Winter Planting on Soil Organic Carbon and Carbon Management Index

To tackle with the problem of prevailing farmland abandonment in winter, 5 treatments includes Chinese milk vetch-double cropping rice (CRR), rape-double cropping rice (RRR), garlic-double cropping rice (GRR), winter crop multiple cropping rotation (ROT), winter fallow control (WRR) were set up. By measuring soil total organic carbon, active organic carbon and its components and calculating the soil carbon pool management index in 0~15 cm and 15~30 cm soil layers in the early and late rice ripening stage. The effects of different winter planting patterns on the changes of soil organic carbon and carbon pool management index were discussed. In order to provide theoretical basis for the optimization and adjustment of winter planting pattern of double cropping rice field in the middle reaches of Yangtze River. The results showed that soil total organic carbon, active organic carbon and its components in different winter cropping patterns were increased, and ROT and CRR treatments were more beneficial to the accumulation of soil total organic carbon, active organic carbon and its components as well as the improvement of soil carbon pool management index, which should be preferred in the adjustment of cropping patterns.

Pyrolyzed Substrates Induce Aromatic Compound Metabolism in the Post-fire Fungus, Pyronema domesticum

Wildfires represent a fundamental and profound disturbance in many ecosystems, and their frequency and severity are increasing in many regions of the world. Fire affects soil by removing carbon in the form of CO2 and transforming remaining surface carbon into pyrolyzed organic matter (PyOM). Fires also generate substantial necromass at depths where the heat kills soil organisms but does not catalyze the formation of PyOM. Pyronema species strongly dominate soil fungal communities within weeks to months after fire. However, the carbon pool (i.e., necromass or PyOM) that fuels their rise in abundance is unknown. We used a Pyronema domesticum isolate from the catastrophic 2013 Rim Fire (CA, United States) to ask whether P. domesticum is capable of metabolizing PyOM. Pyronema domesticum grew readily on agar media where the sole carbon source was PyOM (specifically, pine wood PyOM produced at 750°C). Using RNAseq, we investigated the response of P. domesticum to PyOM and observed a comprehensive induction of genes involved in the metabolism and mineralization of aromatic compounds, typical of those found in PyOM. Lastly, we used 13C-labeled 750°C PyOM to demonstrate that P. domesticum is capable of mineralizing PyOM to CO2. Collectively, our results indicate a robust potential for P. domesticum to liberate carbon from PyOM in post-fire ecosystems and return it to the bioavailable carbon pool.