ancient proteins
Recently Published Documents


TOTAL DOCUMENTS

67
(FIVE YEARS 8)

H-INDEX

20
(FIVE YEARS 0)

eLife ◽  
2021 ◽  
Vol 10 ◽  
Author(s):  
Thanh Thi Vuong-Brender ◽  
Sean Flynn ◽  
Yvonne Vallis ◽  
Mario de Bono

The ubiquitous Ca2+ sensor calmodulin (CaM) binds and regulates many proteins, including ion channels, CaM kinases, and calcineurin, according to Ca2+-CaM levels. What regulates neuronal CaM levels, is, however, unclear. CaM-binding transcription activators (CAMTAs) are ancient proteins expressed broadly in nervous systems and whose loss confers pleiotropic behavioral defects in flies, mice, and humans. Using Caenorhabditis elegans and Drosophila, we show that CAMTAs control neuronal CaM levels. The behavioral and neuronal Ca2+ signaling defects in mutants lacking camt-1, the sole C. elegans CAMTA, can be rescued by supplementing neuronal CaM. CAMT-1 binds multiple sites in the CaM promoter and deleting these sites phenocopies camt-1. Our data suggest CAMTAs mediate a conserved and general mechanism that controls neuronal CaM levels, thereby regulating Ca2+ signaling, physiology, and behavior.



Impact ◽  
2021 ◽  
Vol 2021 (3) ◽  
pp. 86-88
Author(s):  
Takumi Nishiuchi

The study of ancient civilisations enables us to establish an understanding of how societies have changed over thousands of years and helps provide useful context for present-day civilisations, as well as highlighting similarities between past and present civilisations. The large-scale study of proteins – proteomics – is one way that scientists can discover the foodstuffs that ancient civilisations grew and ate and gain interesting insights into what life was like back then. This is done through the identification of proteins in materials found during excavations and is at the heart of the work of Associate Professor Takumi Nishiuchi, Advanced Science Research Center, Kanazawa University, Japan. Through the analysis of ancient proteins, Nishiuchi and his team are stimulating ancient food and using archeological data to envisage the lifestyles of ancient civilisations. The researchers are working at ruins in China and South Korea, as well as at two Japanese ruins in Fukuoka prefecture, with a view to better understanding the propagation of rice food culture and, in the process, providing context to Asia's ongoing food culture. In innovative work involving Orbitrap mass spectrometry, the team has performed protein analysis in plant remains and food crusts found at various sites, which is something that has not been done many times before. The researchers hope their work will lay the foundations for similar studies at sites across the globe, providing insights into other civilisations.



Author(s):  
Nina Sophia Mahlke ◽  
Silvia Renhart ◽  
Dorothea Talaa ◽  
Alexandra Reckert ◽  
Stefanie Ritz-Timme

AbstractAge at death estimation in cases of human skeletal finds is an important task in forensic medicine as well as in anthropology. In forensic medicine, methods based on “molecular clocks” in dental tissues and bone play an increasing role. The question, whether these methods are applicable also in cases with post-depositional intervals far beyond the forensically relevant period, was investigated for two “protein clocks”, the accumulation of D-aspartic acid (D-Asp) and the accumulation of pentosidine (Pen) in dentine. Eight teeth of skeletons from different burial sites in Austria and with post-depositional intervals between c. 1216 and c. 8775 years were analysed. The results of age at death estimation based on D-Asp and Pen in dentine were compared to that derived from a classical morphological examination. Age at death estimation based on D-Asp resulted consistently in false high values. This finding can be explained by a post-mortem accumulation of D-Asp that may be enhanced by protein degradation. In contrast, the Pen-based age estimates fitted well with the morphological age diagnoses. The described effect of post-mortem protein degradation is negligible in forensically relevant time horizons, but not for post-depositional intervals of thousands of years. That means that the “D-Asp clock” loses its functionality with increasing post-depositional intervals, whereas Pen seems to be very stable. The “Pen-clock” may have the potential to become an interesting supplement to the existing repertoire of methods even in cases with extremely long post-depositional intervals. Further investigations have to test this hypothesis.



Author(s):  
Martina Crippa ◽  
Damiano Andreghetti ◽  
Riccardo Capelli ◽  
Guido Tiana

AbstractEnergetic properties of a protein are a major determinant of its evolutionary fitness. Using a reconstruction algorithm, dating the reconstructed proteins and calculating the interaction network between their amino acids through a coevolutionary approach, we studied how the interactions that stabilise 890 proteins, belonging to five families, evolved for billions of years. In particular, we focused our attention on the network of most strongly attractive contacts and on that of poorly optimised, frustrated contacts. Our results support the idea that the cluster of most attractive interactions extends its size along evolutionary time, but from the data, we cannot conclude that protein stability or that the degree of frustration tends always to decrease.



Amino Acids ◽  
2021 ◽  
Vol 53 (2) ◽  
pp. 295-312
Author(s):  
Davide Tanasi ◽  
Annamaria Cucina ◽  
Vincenzo Cunsolo ◽  
Rosaria Saletti ◽  
Antonella Di Francesco ◽  
...  

AbstractMass spectrometry-based approaches have been successfully applied for identifying ancient proteins in bones and other tissues. On the contrary, there are relatively few examples of the successful recovery and identification of archeological protein residues from ceramic artifacts; this is because ceramics contain much lower levels of proteins which are extensively degraded by diagenetic effects. In this paper, we report the results of the characterization of proteins extracted from pottery of the Maltese site of Baħrija, the guide-site for the Baħrija period (half of 9th–second half of eighth century BCE), recently identified as the final part of the Borġ in-Nadur culture. Proteomic data here reported confirm that one of the major issue of these kind of studies is represented by contamination of animal and human agents that may complicate endogenous protein identification and authentication. The samples tested included a small group of ceramic forms, namely three tableware and six coarse ware thought to have been used in food preparation and/or storage. In this context, the limited availability of paleobotanical and archeozoological analyses may be compensated by the outcomes of the first proteomics profiling which, even if obtained on a limited selection of vessels, revealed the centrality of wheat in the diet of the ancient community of Baħrija. The data have been deposited to the ProteomeXchange with identifier < PXD022848 > .



2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Madeleine Bleasdale ◽  
Kristine K. Richter ◽  
Anneke Janzen ◽  
Samantha Brown ◽  
Ashley Scott ◽  
...  

AbstractConsuming the milk of other species is a unique adaptation of Homo sapiens, with implications for health, birth spacing and evolution. Key questions nonetheless remain regarding the origins of dairying and its relationship to the genetically-determined ability to drink milk into adulthood through lactase persistence (LP). As a major centre of LP diversity, Africa is of significant interest to the evolution of dairying. Here we report proteomic evidence for milk consumption in ancient Africa. Using liquid chromatography tandem mass spectrometry (LC-MS/MS) we identify dairy proteins in human dental calculus from northeastern Africa, directly demonstrating milk consumption at least six millennia ago. Our findings indicate that pastoralist groups were drinking milk as soon as herding spread into eastern Africa, at a time when the genetic adaptation for milk digestion was absent or rare. Our study links LP status in specific ancient individuals with direct evidence for their consumption of dairy products.



2021 ◽  
Vol 7 (3) ◽  
pp. eabb9314
Author(s):  
Jessica Hendy

The analysis of ancient proteins from paleontological, archeological, and historic materials is revealing insights into past subsistence practices, patterns of health and disease, evolution and phylogeny, and past environments. This review tracks the development of this field, discusses some of the major methodological strategies used, and synthesizes recent developments in archeological applications of ancient protein analysis. Moreover, this review highlights some of the challenges faced by the field and potential future directions, arguing that the development of minimally invasive or nondestructive techniques, strategies for protein authentication, and the integration of ancient protein analysis with other biomolecular techniques are important research strategies as this field grows.





ACS Catalysis ◽  
2020 ◽  
Vol 10 (9) ◽  
pp. 4863-4870 ◽  
Author(s):  
Jasmine M. Gardner ◽  
Michal Biler ◽  
Valeria A. Risso ◽  
Jose M. Sanchez-Ruiz ◽  
Shina C. L. Kamerlin


Sign in / Sign up

Export Citation Format

Share Document