Water Kefir and Derived Pasteurized Beverages Modulate Gut Microbiota, Intestinal Permeability and Cytokine Production In Vitro

Fermentation is an ancient food preservation process, and fermented products have been traditionally consumed in different cultures worldwide over the years. The interplay between human gut microbiota, diet and host health is widely recognized. Diet is one of the main factors modulating gut microbiota potentially with beneficial effects on human health. Fermented dairy products have received much attention, but other sources of probiotic delivery through food received far less attention. In this research, a combination of in vitro tools mimicking colonic fermentation and the intestinal epithelium have been applied to study the effect of different pasteurized and non-pasteurized water kefir products on gut microbiota, epithelial barrier function and immunomodulation. Water kefir increased beneficial short-chain fatty acid production at the microbial level, reduced detrimental proteolytic fermentation compounds and increased Bifidobacterium genus abundance. The observed benefits are enhanced by pasteurization. Pasteurized products also had a significant effect at the host level, improving inflammation-induced intestinal epithelial barrier disruption and increasing IL-10 and IL-1β compared to the control condition. Our data support the potential health benefits of water kefir and demonstrate that pasteurization, performed to prolong shelf life and stability of the product, also enhanced these benefits.

In Silico Bioactive Peptide Prediction from The Enzymatic Hydrolysates of Edible Seaweed Rubisco Large Chain

Seaweeds are one of the ancient food supplements on Earth. Especially Asian countries use seaweeds as the fundamental ingredient in their cuisine. Seaweeds are photosynthetic organisms living in aquatic ecosystems and in the coastal territories. Seaweeds out of farm areas are frequently observed as coastal wastes. However, seaweeds are outstanding sources for bioactive substances and investigation bioactive properties of seaweed RuBisCO has never been done. RuBisCO is the most abundant protein on Earth but a vast amount of RuBisCO goes through waste. In this study, bioactive peptide prediction of frequently consumed seaweed RuBisCO proteins were analyzed in silico to identify possible bioactive peptides as substitute or support for grain, meat, and dairy based bioactive peptides. A huge portion of peptides were di-, tri- peptides with IC50 values less than 300 µM according to the comparison of BIOPEP database. Including gastric digestion, more than half of the peptides showed DDP-IV and ACE inhibitory activity followed by antioxidant properties. Also, novel antiinflammatory and anti-cancer peptides were found through in silico analysis.

Echoes of the late Pleistocene in a novel trophic cascade between cougars and feral donkeys

Introduced large herbivores have partly filled ecological gaps formed in the late Pleistocene, when many of the Earth’s megafauna were driven extinct. However, surviving predators are widely considered unable to influence introduced megafauna, leading them to exert unusually strong herbivory and disturbance-related effects. We report on a behaviorally-mediated trophic cascade between cougars (Puma concolor) and feral donkeys (Equus africanus asinus) at desert wetlands in North America. In response to predation of juveniles, donkeys shifted from nocturnal to almost exclusively diurnal, thereby avoiding peaks in cougar activity. Furthermore, donkeys reduced the time they spent at desert wetlands by 87%: from 5.5 hours a day to 0.7 hours at sites with predation. These shifts in activity were associated with increased activity and richness of other mammal species and reduced disturbance and herbivory-related effects on these ecologically-distinct wetland ecosystems, including 49% fewer trails, 35% less trampled bare ground, and 227% more canopy cover. Cougar predation on introduced donkeys rewires an ancient food web, with diverse implications for modern ecosystems.

Reconstruction of the eating habits of the Japonesians by proteomics

The study of ancient civilisations enables us to establish an understanding of how societies have changed over thousands of years and helps provide useful context for present-day civilisations, as well as highlighting similarities between past and present civilisations. The large-scale study of proteins – proteomics – is one way that scientists can discover the foodstuffs that ancient civilisations grew and ate and gain interesting insights into what life was like back then. This is done through the identification of proteins in materials found during excavations and is at the heart of the work of Associate Professor Takumi Nishiuchi, Advanced Science Research Center, Kanazawa University, Japan. Through the analysis of ancient proteins, Nishiuchi and his team are stimulating ancient food and using archeological data to envisage the lifestyles of ancient civilisations. The researchers are working at ruins in China and South Korea, as well as at two Japanese ruins in Fukuoka prefecture, with a view to better understanding the propagation of rice food culture and, in the process, providing context to Asia's ongoing food culture. In innovative work involving Orbitrap mass spectrometry, the team has performed protein analysis in plant remains and food crusts found at various sites, which is something that has not been done many times before. The researchers hope their work will lay the foundations for similar studies at sites across the globe, providing insights into other civilisations.

Disentangling ecological and taphonomic signals in ancient food webs

Analyses of ancient food webs reveal important paleoecological processes and responses to a range of perturbations throughout Earth's history, such as climate change. These responses can inform our forecasts of future biotic responses to similar perturbations. However, previous analyses of ancient food webs rarely accounted for key differences between modern and ancient community data, particularly selective loss of soft-bodied taxa during fossilization. To consider how fossilization impacts inferences of ancient community structure, we (1) analyzed node-level attributes to identify correlations between ecological roles and fossilization potential and (2) applied selective information loss procedures to food web data for extant systems. We found that selective loss of soft-bodied organisms has predictable effects on the trophic structure of “artificially fossilized” food webs because these organisms occupy unique, consistent food web positions. Fossilized food webs misleadingly appear less stable (i.e., more prone to trophic cascades), with less predation and an overrepresentation of generalist consumers. We also found that ecological differences between soft- and hard-bodied taxa—indicated by distinct positions in modern food webs—are recorded in an early Eocene web, but not in Cambrian webs. This suggests that ecological differences between the groups have existed for ≥48 Myr. Our results indicate that accounting for soft-bodied taxa is vital for accurate depictions of ancient food webs. However, the consistency of information loss trends across the analyzed food webs means it is possible to predict how the selective loss of soft-bodied taxa affects food web metrics, which can permit better modeling of ancient communities.

New genus of the tribe Ceutorhynchini (Coleoptera: Curculionidae) from the late Oligocene of Enspel, southwestern Germany, with a remark on the role of weevils in the ancient food web

The new weevil genus Igneonasus gen. nov. (type species: I. rudolphi sp. nov.) of the tribe Ceutorhynchini (Curculionidae: Conoderinae: Ceutorhynchitae) is described from the late Oligocene of Fossillagerstätte Enspel, Germany. The new genus differs from the similar genus Stenocarus Thomson, 1859 in the anterior margin of the pronotum, which is not raised, a pronotum without tubercles on the sides, and a femur without teeth. This weevil is the largest representative of this supertribe and the first fossil Curculionidae species described from the paleolake Enspel. In this ancient ecosystem, weevils were at least sometimes an important food resource for the cyprinid fish Palaeorutilus enspelensis.

Edible Wild Flowers: An Innovative but Ancient Food

Edible flowers are often mentioned in connection with biologically active substances and their presence is today frequent in supermarkets. The main factor that determines their use is their appearance, so particular attention must be paid to the shelf life. Flowers must be protected from desiccation and their quality must be preserved. Thus, the aims of this study were to evaluate the shelf life of the spontaneous edible flowers Glebionis segetum, Malva sylvestris, and Tropaeolum majus and Papaver rhoeas, and to detect the presence of major polyphenols. The flowers were packaged with polypropylene (PP) bags and then stored in a refrigerator at +4 ± 1 °C. Samples were tested in the dark and in the presence of light for 12 days. Gas composition, weight loss, and color were registered once the packages were opened; antioxidant activity, total phenols, and enzymatic analyses were performed. The light has a negative influence, the weight loss was limited, and the gas composition did not change significantly during shelf life, with variation in the studied samples. The highest variation in color parameters was found in control samples, whereas the extracts from G. segetum showed the highest antioxidant activity and polyphenol content; in addition, it was the most resistant but also had the highest weight loss. Furthermore, enzymatic results indicated the PP was the best packaging film if used in the dark. P. rhoeas was characterized by extreme fragility, M. sylvestris was not able to tolerate low temperatures, and T. majus changed its color progressively with time.