The Effect of Endophytic Talaromyces pinophilus on Growth, Absorption and Accumulation of Heavy Metals of Triticum aestivum Grown on Sandy Soil Amended by Sewage Sludge

Sewage sludge improves agricultural soil and plant growth, but there are risks associated with its use, including high heavy metal content. In this study, experiments were carried out to investigate the role of endophytic Talaromyces pinophilus MW695526 on the growth of Triticum aestivum cultivated in soil amended with sewage sludge and its phytoremediation ability. T. pinophilus could produce gibberellic acid (GA) and stimulate T. aestivum to accumulate GA. The results showed that inoculation with T. pinophilus boosted plant growth criteria, photosynthetic pigments, osmolytes (soluble proteins, soluble sugars and total amino acids), enzymatic antioxidants (catalase, superoxide dismutase and peroxidase), K, Ca and Mg. On the other hand, it reduced Na, Na/K ratio, Cd, Ni, Cu and Zn in the growth media as well as in the shoot and root of T. aestivum. The results suggest that endophytic T. pinophilus can work as a barrier to reduce the absorption of heavy metals in T. aestivum cultivated in soil amended with sewage sludge.

IMPACTO DEL CAMBIO EN EL MANEJO DEL CULTIVO DE TRIGO DE CONVENCIONAL A ORGÁNICO SOBRE LAS COMUNIDADES FÚNGICAS CULTIVABLES DEL SUELO EN EL VALLE DEL YAQUI, MÉXICO

El uso de fertilizantes orgánicos en el Valle del Yaqui tiende a reducir la degradación del suelo en la producción de trigo, sin embargo, todavía no se han evaluado los impactos biológicos de esta práctica en el suelo. El objetivo de este experimento fue cuantificar el impacto del cambio en el manejo del cultivo de trigo, de convencional a orgánico, sobre la reducción de la degradación fúngica del suelo, por medio del análisis de sus poblaciones fúngicas cultivables y su diversidad. La hipótesis fue que la aportación de fertilizantes orgánicos al cultivo de trigo produce cambios positivos en las propiedades fisicoquímicas del suelo, lo cual beneficia a las poblaciones de hongos, y disminuye su degradación. Tres campos comerciales de trigo se seleccionaron con 1) fertilización sintética convencional (SF), 2) fertilización sintética más fertilización orgánica (SF+OF) y 3) sólo fertilización orgánica (OF). Los sitios de estudio bajo OF y SF+OF vs. SF mostraron mayores, contenidos de materia orgánica (1.5, 1.6 vs. 0.45%), población de hongos (1.7x105, 9.1x104 vs. 1.6x104 CFU g-1 de suelo seco) y diversidad (12, 12 vs. 2). Con base en el análisis filogenético, se aislaron 9, 11 y 2 géneros de hongos de los sitios de estudio bajo fertilización orgánica, sintética + orgánica y sintética, respectivamente. La diversidad metabólica de las cepas fúngicas obtenidas mostró que la producción de indoles osciló entre 0.5 y 65.4 μg mL-1; el hongo Stachybotrys sp. TSM35 fue el productor de indoles mayor. La solubilización de fosfatos mayor (21%) se observó en la cepa Talaromyces pinophilus TSO42 y la cepa con producción de sideróforos mayor (33.9%) fue Volutella ciliata TSM43. Además, el 60% de las cepas mostraron riesgos potenciales para la salud (α- o β-hemólisis). La rotación de convencional a orgánico en el cultivo del trigo logró cambios positivos en las propiedades fisicoquímicas del suelo, en la diversidad y características metabólicas de las poblaciones fúngicas cultivable. El control de la calidad microbiológica de los fertilizantes orgánicos se necesita para evitar riesgos microbiológicos en los sistemas agronómicos, causados por la introducción de cepas con potencial patogénico.

Effect of soil conditioner enriched with biofertilizers to improve soil fertility and maize (Zea mays L.) growth on andisols Sinabung area

<p>Andisol soil contains a lot of heavy metals Al and Fe, which results in P being unavailable to plants and can cause low soil pH, this will inhibit plant growth. One effort that can be made to increase the availability of nutrients in the soil is by utilizing soil enhancing ingredients enriched with biological fertilizers. The research design used was factorial randomized block design (RBD) consisting of: Factor I: (biological fertilizers) M0 = without application, M1 = <em>Talaromyces pinophilus </em>(Hedgecock), M2 = <em>Azotobacter</em> sp, M3 = Mycorrhizae, M4 = <em>Talaromyces pinophilus</em> + mycorrhizae + <em>Azotobacter </em>sp. Factor II Soil enhancing ingredients, namely P0 = Without Soil Improvement, K1 = Zeolite 50 g, K2 = Humic acid 50 ml, K3 = compost / manure fertilizer for agriculture 50 g. From the results the combination of microbial treatment and soil conditioner can increase nitrogen in the soil by 2-40.81 %, cation exchange capacity by 1.7-44.29 % and P available by 1.3-49.36 %. Soil conditioner combined with biological fertilizers can improve soil quality in general, the best treatment is a combination treatment of <em>T.</em> <em>pinophilus</em> + mycorrhizae + <em>Azotobacter </em>sp. with coffee skin (M4P4).</p>

Keefektifan Bioinsektisida Berbasis Cendawan Entomopatogen Talaromyces pinophilus dan Minyak Nabati terhadap Hama Penggerek Buah Kopi

<em>The coffee berry borer (CBB) Hypothenemus hampei (Coleoptera: Curculionidae: Scolytinae), is one of the main pests that cause yield losses in coffee plants. This pest directly attacks the coffee berries, both immature and ripe. The entomopathogenic fungi is one of the potential biological agents for CBB, environmentally friendly and does not kill non-target organisms. The study was conducted at the Integrated Laboratory, Industrial and Beverage Crop Research Institute, from June to October 2018. The aim of this study was to examine the effectiveness of bioinsecticide based on entomopathogenic fungus of Talaromyces pinophilus (EFTP) and vegetable oil on CBB. The completely randomized design with 31 treatments and 3 replications was used in this study. The 31^th of treatments consisted of 9 formulas of bioinsecticide based on EFTP and vegetable oil, control-positive (bioinsecticide based on EFTP and water), and control- negative (Klorpirifos insecticide). Variables observed were the activities of EFTP enzyme, effectiveness of vegetable oil as a carrier material, mortality of CBB beetles, percentage of CBB attacks, percentage of hollow berries, and number of holes per 10 coffee berries. Results showed that the formula of bioinsecticide based on EFTP produce the chitinase and protease enzymes which were potential as biological agents to control CBB in vitro. Soybean oil is the most effective as a carrier material in formulating EFTP. The formula of S69MK30 and S79MK20 in concentration of 7.50% respectively have the highest potential as biological agents to control CBB. Other potential formula is S69MKT30 in concentration of 7.50%.</em>