Relationships among Indicators of Metabolism, Mammary Health and the Microbiomes of Periparturient Holstein Cows

During the period called “transition”, from the ceasing of milk production to the reestablishment of full milk production, it is postulated that the microbiota of cows undergo changes in composition driven by the fluxes in systemic energetics and that these changes appear to impact the health of cows. The primary objective of this study was to document the make-up of the microbiota in the mammary gland compared with those in the vagina and in feces in an attempt to determine any correlations between the composition of the microbiota, the impact of blood indicators of energetic metabolites and the health of the mammary gland at the time of calving. Samples were collected from 20 Holstein dairy cows immediately following calving to assess their general health and measure the microbiomes associated with each cow using 16S rRNA sequencing. The results indicated that the microbiomes found within each maternal niche were different. A set of significant negative associations between the blood energetic biomarkers (NEFAs, BHB, triglycerides and cholesterol) and the taxa Pseudomonas, Christensenellaceae and Methanobrevibacter were observed in this study. In contrast, Escherichia and Romboutsia were positively correlated with the same energetic metabolites. Therefore, it was concluded that there appears to be a set of relationships between the microorganisms that colonize several niches of cows and the sufficiency of systemic energy metabolism. Furthermore, both the microbiome and energy dynamics impact the health of the mammary gland of the host.

Effect of Immobilization on Changes in Blood Glucose and Cholesterol Concentration in the Examined Mice – Preliminary Studies

Introduction: Immobilization in animals may be an analogue of strong neurogenic stress in humans, in which an adaptive reaction aimed at energy mobilization of the system seems seemingly insignificant. The animal does not move, so it does not lose energy. However, there is an energy mobilization of the organism in which most regulatory organs and systems participate. Aim: To assess the extent of possible changes in glucose and plasma cholesterol concentration in mice subjected to immobilization. Material and Methods: The study was conducted on 40 female Swiss mice at the age of 12 weeks unselected and selected for a high rate of weight gain. Plasma concentrations of glucose and total cholesterol were determined. Results: Immobilization caused a significant reduction in glucose concentration (83%) and a significant increase in cholesterol (121%) in the plasma of mice selected in comparison to the control group. [From the analysis of multidirectional variance the significance of statistically confirmed differences induced by selection was revealed in relation to glucose [F = 21.81] and plasma cholesterol [F = 45.63]. Conclusions: The selection for high body weight had a significant effect on the level of the examined indicators. This may indicate that selected mice obtained greater protein gain in the form of larger body increases, with lower glucose levels, were forced to work more economically at the cellular level, managing their structural and energetic metabolites more carefully.

Aerobic exercise produces changes in plasma IL-6 but not IL-1b in four-beat gaited horses

Exercise is an important stressor and is correlated with cytokine production in several tissues. There is little information about changes in interleukin (IL)-6 and IL-1b in four-beat gaited horses during a typical aerobic exercise challenge. The objective of this research was to characterise changes in plasma IL-6, IL-1b, cortisol and other blood biomarkers in four-beat gaited horses after a marcha simulation test. We subjected 12 fit horses (approximately 5 years old and 390 kg) to the marcha simulation test (MST) (i.e. a 10 min warm-up, 30 min at marcha and 15 min cool down). Blood samples were collected before the MST, immediately after the MST and 15 and 120 min after the MST (i.e. recovery). Results were analysed with One Way Repeated Measures ANOVA and a Tukey tests with P≤0.05 and Pearson correlation test. The highest value of plasma IL-6 was observed immediately after MST (6.85 pg/ml) (P≥0.05). IL-1 and cortisol did not change (P≥0.05). However, creatine kinase (CK) and aspartate aminotransferase, nonesterified fatty acid (NEFA), glycerol, total cholesterol and glucose were elevated immediately after the MST and at 15 min after the MST (P≤0.05). No change was observed in alanine aminotransferase and triglycerides (P>0.05). Medium correlation was observed between NEFA and glycerol (R=0.64) and glycerol and triglycerides (R=0.50). In conclusion, an increase in IL-6 immediately that occurred after marcha simulation test was associated with elevated concentrations of several energetic metabolites (NEFA, glycerol and glucose). This metabolic adaption may contributed to the horses’ performance during their typical aerobic exercise and was not associated with significant elevation in IL-1b, CK, and cortisol in healthy trained four-beat gaited horses.