Preventif TB Paru Melalui Pengobatan, Informasi dan Edukasi Kepada Masyarakat di Wilayah Kerja Puskesmas Rejosari

Tuberculosis is an infectious disease caused by germs Mycobacterium tuberculosis. There are several species Mycobacterium, among others : M. tuberculosis, M. africanum, M.bovis, M. Laprae dsb. Which is also known as acid resistant bacteria (BTA). Mycobacterium group other than Mycobacterium tuberculosis which can cause distruption of the airways known as MOTT (Mycobacterium Other Than Tuberculosis) which sometime can interfere with the diagnosis of TBC treatment. PKM’s aim is to increase peoples’s knowledge with information and education to the public as a preventive measure against TBC. Methods of health counseling education about TB prevention through information and education to the public. Target community PKM is people in working area Puskesmas Rejosari Tenayan Raya sub-district, Pekanbaru city. The activity was carried out on 12 December 2019. The results of the PKM activity found that out of 25 people participating in the activity, 80% of the community knew about TBc disease and 70% understood how to prevent and treat TBC. It is recommended the puskesmas rejo sari especially the part promkes to intensify provide education and information related to TB disease and the way prevented TB disease also can be eradikasi.

Management of infections with Mycobacterium other than tuberculosis as a complication of surgical procedures

Background: Atypical Mycobacterium other than tuberculosis (MOTT) have emerged as significant human pathogens, causing post-surgical wound infections. The aim of this study is to assess the causative organisms of such infection and their treatment response.Methods: After matching the criteria, 28 cases, were taken for this prospective, single center, observational study. The diagnosis was confirmed by bacterial culture.Results: Among 28 patients, 16 were females (median age of 45.5 year). Patients had undergone laparoscopic cholecystectomy (n=13), laparoscopic appendicectomy (n=3), laparoscopic hernioplasty (n=2), open appendicectomy (n=2), open mesh hernioplasty (n=7), exploratory laparotomy (n=1). No major comorbidities or immunosuppression was identified. All patients were initially treated with repeated incision and drainage and started conventional antibiotics until culture and sensitivity report was available. All except one patient had culture confirmed MOTT infection. Combination antibiotics (clarithromycin, linezolid and ofloxacin) given for initial 3 months. 12 well responded within 3 months. 9 required additional few months to get complete cure. 4 patients cured after 6 months of treatment and 3 patients did not come for follow up.Conclusions: Delayed onset chronic wound infection by atypical mycobacteria is preventable. These organisms are not responsive to conventional antitubercular drugs but to specific drug regimens.

Mutation in MPT64 gene influencing diagnostic accuracy of SD Bioline assay (capilia)

Background Success of India’s TB control program relies on rapid case detection, monitoring, care and treatment of drug resistance. Patients on multidrug resistance (MDR) treatment are monitored by follow up cultures. Discordant results (culture and smear positive while capilia negative) are usually declared negative Mycobacterium tuberculosis complex (MTBC). This study was designed to understand the possible causes of discordant results. Methods The capilia kit was evaluated to test its utility among 4737 follow up MDR patients enrolled during a period of 1 year. A total of 889 were liquid culture positive, 3375 were negative and 473 were contaminated. Of the 889 cultures positive, 829 were found positive by ZN smear, capilia test and MTBDR plus assay. The cultures which gave a positive result on Mycobacterium Growth Indicator Tube 960 (MGIT 960) and ZN smear but were negative on capilia test with no growth on Brain Heart Infusion agar (BHI) were included in this study. The conflicting results of capilia were compared with other molecular techniques; MTBDR plus assay and DNA sequence analysis of MPT64 gene. Results Out of 889 culture positive, 60 (6.7%) were found positive on liquid culture and ZN smear but were negative on capilia. Of these 60 cultures, 10 (16.7%) were found positive by both MTBDR plus assay and PCR. The sequencing analysis revealed that all of the capilia negative isolates had mutations within the MPT64 gene. Conclusion Re-evaluation of culture positive but capilia negative isolates should be done before declaring them as Mycobacterium other than tuberculosis (MOTT) because such cases can act as chronic carriers of TB in the population which can lead to the rise of this lethal disease.