Supply-Demand Matching of Medical Services in Haikou Under the Background of Hierarchical Diagnosis and Treatment - Based on Didi Travel Data

Background Implementation of the Healthy China Strategy and the hierarchical diagnosis and treatment system has injected new vitality into medical services. Given the insufficient supply of medical services and increasing demand for medical treatment, exploring the supply-demand pattern of medical services has become an urgent theoretical and practical problem to be solved. The equity of healthcare facilities has received widespread attention, but due to limited data, there is little research on the supply-demand pattern of medical services. This study focuses on evaluating the supply-demand matching pattern of medical services at different levels in Haikou City with big geographic data and promoting the realization of a balance between medical supply and demand. Methods This study utilizes spatial data of medical institutions, didi travel data, and population density data. Firstly, use the two-step floating catchment area method and GIS spatial analysis to explore characteristics of the supply-demand patterns of medical services at different levels in Haikou. Secondly, based on didi travel data, mine residents' demand for medical treatment. Then combined with population density data, divide supply-demand matching of medical institutions into four types. Finally, propose optimization strategies for the problems. Results The accessibility pattern of high-level medical institutions in Haikou presents high in the north and low in the south. The accessibility pattern of low-level medical institutions is the opposite. High-level medical institutions have a strong need for medical treatment, which is less hampered by distance. The healthcare demand of low-level medical institutions is small, and they mainly are medium- and short-distance medical travel. The types of medical services at different levels are mainly "low supply - low demand" and "high supply - low demand" types. Conclusions Medical services at different levels in Haikou are mainly in supply-demand imbalance. Therefore, we put forward optimization strategies to promote the equity of primary medical services, such as propelling the establishment and improvement of the hierarchical diagnosis and treatment system, building a new model of medical and health service supply, and strengthening balanced coverage of primary medical institutions. The mining of big geographic data is beneficial to alleviate the contradiction between medical supply and demand, although the data and methods need to be improved.

APEC: an accesson-based method for single-cell chromatin accessibility analysis

ABSTRACTThe development of sequencing technologies has promoted the survey of genome-wide chromatin accessibility at single-cell resolution; however, comprehensive analysis of single-cell epigenomic profiles remains a challenge. Here, we introduce an accessibility pattern-based epigenomic clustering (APEC) method, which classifies each individual cell by groups of accessible regions with synergistic signal patterns termed “accessons”. By integrating with other analytical tools, this python-based APEC package greatly improves the accuracy of unsupervised single-cell clustering for many different public data sets. APEC also predicts gene expressions, identifies significant differential enriched motifs, discovers super enhancers, and projects pseudotime trajectories. Furthermore, we adopted a fluorescent tagmentation-based single-cell ATAC-seq technique (ftATAC-seq) to investigated the per cell regulome dynamics of mouse thymocytes. Associated with ftATAC-seq, APEC revealed a detailed epigenomic heterogeneity of thymocytes, characterized the developmental trajectory and predicted the regulators that control the stages of maturation process. Overall, this work illustrates a powerful approach to study single-cell epigenomic heterogeneity and regulome dynamics.

Study of the Impact of a High-Speed Railway Opening on China’s Accessibility Pattern and Spatial Equality

China’s high-speed rail was inaugurated in 2008; it has greatly improved accessibility, and reduced the time required to travel between cities, but at the same time, has caused an unfair distribution of accessibility levels. Therefore, this paper analyzes urban traffic roads and socio-economic statistics, using network analysis methods, accessibility coefficients of variation, and social demand indexes to explore the spatial and temporal characteristics of transport accessibility and spatial equity in China. By 2015, the national transport accessibility level will form a new pattern of “corridors” and “islands”, centered on high-speed rail lines and sites. Additionally, the opening of high-speed railways has improved, to a certain extent, the inter-regional accessibility balance, and increased accessibility from high-speed railway sites to non-site cities. Spatial equality was also analyzed using the accessibility coefficient and social demand index. In conclusion, studying accessibility and spatial equity plays an important role in the rational planning of urban land resources and transportation.

Suppression of the GLUT4 adaptive response to exercise in fructose-fed rats

Exercise-induced increase in skeletal muscle GLUT4 expression is associated with hyperacetylation of histone H3 within a 350-bp DNA region surrounding the myocyte enhancer factor 2 (MEF2) element on the Glut4 promoter and increased binding of MEF2A. Previous studies have hypothesized that the increase in MEF2A binding is a result of improved accessibility of this DNA segment. Here, we investigated the impact of fructose consumption on exercise-induced GLUT4 adaptive response and directly measured the accessibility of the above segment to nucleases. Male Wistar rats ( n = 30) were fed standard chow or chow + 10% fructose or maltodextrin drinks ad libitum for 13 days. In the last 6 days five animals per group performed 3 × 17-min bouts of intermittent swimming daily and five remained untrained. Triceps muscles were harvested and used to measure 1) GLUT4, pAMPK, and HDAC5 contents by Western blot, 2) accessibility of the DNA segment from intact nuclei using nuclease accessibility assays, 3) acetylation level of histone H3 and bound MEF2A by ChIP assays, and 4) glycogen content. Swim training increased GLUT4 content by ∼66% ( P < 0.05) but fructose and maltodextrin feeding suppressed the adaptation. Accessibility of the DNA region to MNase and DNase I was significantly increased by swimming (∼2.75- and 5.75-fold, respectively) but was also suppressed in trained rats that consumed fructose or maltodextrin. Histone H3 acetylation and MEF2A binding paralleled the accessibility pattern. These findings indicate that both fructose and maltodextrin modulate the GLUT4 adaptive response to exercise by mechanisms involving chromatin remodeling at the Glut4 promoter.

Aromatic π-networks in Sm/LSm protein interfaces

In this work, we have analyzed the influence of ?-? interactions on stability and properties of Sm/LSm assemblies. Phe residues were found to be involved in ?-? interactions much more frequently than Tyr or His. Similarly, the Phe-Phe ?-? interacting pair had the highest frequency of occurrence. Furthermore, a significant number of ?-networks were observed at the interface of Sm/LSm proteins. Generally speaking, the distance between the interacting pairs was in the range of 5-6 ?. 3? and 7?-networks were found to frequently have plane-plane angles less than 60?. Solvent accessibility pattern of Sm/LSm proteins revealed that all of the interacting residues were from buried areas. Moreover, most of the ?-? interacting residues of Sm/LSm proteins were evolutionary conserved and were in the strand regions. A high percentage of these residues could be considered as stabilization centers that (significantly) contribute to the net stability of Sm/LSm proteins.

FRACTAL PATTERN IN SPATIAL STRUCTURE OF URBAN ROAD NETWORKS

In this paper, we investigate the fractal pattern in spatial structure of urban road networks. By introducing sub-domain, an improved box-counting algorithm is proposed to obtain the fractal pattern. Numerical experiments explore the realistic urban road network at Dalian city in China. In order to clearly show the performance of our proposed box-counting algorithm, two other measures for urban road networks, i.e., density pattern and accessibility pattern are introduced, compared and discussed.