Male and Female Cloacal Anatomy of the Fischer’s Clawed Salamander, Onychodactylus fischeri (Caudata, Hynobiidae)

The cloaca of salamanders is a complex organ with exocrine glands involved in the production of sex pheromones, spermatophores, and storage of sperm. Since the cloaca provides reproductive functions, its signs are important for phylogenetic analysis in the evolutionary biology of tailed amphibians. For clarification of intrafamilial variation of cloacal characteristics in hynobiids, we studied the anatomy of male and female cloacae of Onychodactylus fischeri via histological, histochemical, and 3D-reconstruction methods. Males and females had ciliated cloacal linings and with sexual dimorphism in cloacal conformation and cloacal glands. As in other males and females of hynobiids, females of O. fischeri possessed only ventral glands, secreting neutral glycoproteins. In contrast, males of this species had three types of the cloacal glands. Glands «B» were like ventral glands of females and other hynobiids, while glands «A» and «C» had different histochemical and morphological characteristics. As our results are generally consistent with the data for the related species O. japonicus, these characteristics of the male and female cloacal anatomy may be common to all species of the genus Onychodactylus. The presence of three types of unique cloacal glands in males distinguishes Onychodactylus from all other hynobiids and salamanders.

New Eremidrilus species (Clitellata: Lumbriculidae) from western North America. Part 1, species with two spermathecal segments

Four new species of the lumbriculid genus Eremidrilus Fend & Rodriguez, 2003 are described from Idaho, Montana, and New Mexico. The new species all have a filiform proboscis, club-shaped atria in X, each with two functional vasa deferentia, and spermathecae paired in both XI and XII. Male pores of Eremidrilus artzaini n. sp., E. humboldti n. sp., and E. gilita n. sp. are all associated with distinctive porophores, but these structures are absent in E. montanensis n. sp. The new species are easily distinguished from California Eremidrilus species, all of which have spermathecae only in XI. New observations of type specimens of Eremidrilus allegheniensis (Cook, 1971), a species with two spermathecal segments, known only from eastern USA, confirm differences in atrial morphology, and also document unusual spermathecal porophores and ventral glands.

Ultrastructure of the prosomal gland complex in unfed larvae of the fresh-water mite Limnesia maculata (Müller, 1776) (Acariformes, Limnesiidae)

The salivary and coxal glands in unfed larvae of the fresh-water mite Limnesia maculata (Müller, 1776) (Acariformes, Limnesiidae) were studied using light and electron microscopy. Three pairs of the salivary and one pair of the coxal glands constitute the prosomal gland complex. The salivary glands pertain to simple alveolar glands consisting of the single acinus with prismatic cells arranged around the intra-alveolar lumen. Owing to their spatial distribution, the salivary glands may be termed as medial, ventral and lateral. While the ventral and lateral glands are arranged symmetrically, the medial glands are disposed one after another at the frontal wall of the synganglion (brain). The medial and the ventral glands possess mostly electron-dense secretory granules supposedly of protein nature. The lateral glands show variable organization mainly having electron-lucent vacuoles. No well pronounced rough endoplasmic reticulum and Golgi bodies were discernible in the salivary glands. The coxal glands are well developed and composed of (i) the main portion (the so-called labyrinth) including at least two tightly opposed loops and extending backward along the brain, and (ii) the very short anterior portion/end piece transforming anteriorly into the terminal sac/bladder. The cells of the main portion are provided with apical microvilli, whereas the basal infolding/basal labyrinth is lacking. The cells of the anterior portion do not show the apical microvilli and the basal infoldings. The terminal sac is lined by a cuticle and has an ectodermal origin. The ducts of the salivary glands come together at the dorsal aspect of the bladder, to which they open forming the common podocephalic duct anteriorly. The latter opens on each side into the subcheliceral space. The well-developed podocephalic system suggests its active role in the living processes such as feeding and ion-water balance.

A new soil centipede from South-East Asia with a unique arrangement of ventral glands, and a revised synopsis of Gonibregmatidae (Chilopoda, Geophilomorpha)

A new gonibregmatid centipede,Vinaphilusunicusgen. n.,sp. n., is described based on two females from a single location in northern Vietnam. The new genus and species are distinguished mainly by the arrangement of the ventral pore-fields, which is unique among all Chilopoda. A critically revised synopsis of the Gonibregmatidae is also given. In particular, three species are provisionally recognized inHimatosomaPocock, 1891:H.bidivisumSilvestri, 1919,H.porosumPocock, 1891 (=H.typicumtridivisumSilvestri, 1919,syn. n.), andH.typicumPocock, 1891. The generaDschangelophilusVerhoeff, 1937 andTweediphilusVerhoeff, 1937, with their speciesD.coloratusVerhoeff, 1937 andT.malaccanusVerhoeff, 1937, are moved to the Gonibregmatidae, whereasGeoporophilusaporusAttems, 1930 is moved to the Oryidae asOrphnaeusaporus(Attems, 1930),comb. n.

Ultrastructural aspects of the oesophageal and reproductive systems of the equine parasiteStrongylus vulgaris

The ultrastructure of the dorsal oesophageal gland ampulla and its relationship with the oesophagus, oesophageal ultrastructure, and control mechanisms in oesophageal activity were studied. Terminal ducts of the sub-ventral glands open through the oesophageal crown at the base of the buccal cavity. The terminal duct of the dorsal oesophageal gland running through the dorsal gutter opens to the exterior at the rim ‘groove’ of the buccal capsule. The posterior oesophageal region is clavate and the cuticle of the lumen folds to form outlet valves, ‘valvulae’. An inconspicuous oesophago-intestinal valve (three lobes) connects oesophagus and intestine and is visualized in the open and shut position. In the female reproductive tract, with the exception of the uterus, the cells lie on a thick, irregular (convoluted) basal lamina. The apical plasma membrane of the uterus, and seminal receptacle, extend into the lumen by microvilli-like projections with which spermatozoa make intimate contact. The lumen of the uterus is filled with oocytes, fertilized and unfertilized. Testicular cells have two parts linked by a rachis. Spermatocytes are elongated with a large nucleus, distinct nuclear membrane, and many granules. The apical membrane of the rachis forms long microvilli-like projections with balloon-like tips. The amoeboid spermatozoa contain membrane specializations, a nucleus devoid of a membrane, and are enclosed by a pseudopodial-like extension.