Local Anesthetic Efficacy of Tramadol Hydrochloride with Adrenaline in the Extraction of Maxillary Teeth

Aims: To access the local anaesthetic effect of tramadol hydrochloride with adrenaline and its applicability in the extraction of maxillary teeth. Methodology: 100 patients were selected from the outpatient department (OPD) of oral and maxillofacial surgery, who needed extraction of any maxillary teeth and were under ASA – I category, and Using software SPSS Version 16 statistical analysis was done. Results: Tramadol hydrochloride 50mg with adrenaline 0.0225mg exhibits a local anaesthetic effect that enables successful and painless extraction of maxillary teeth. Conclusion: Tramadol hydrochloride does exhibit local anaesthetic effect and can be used as an alternative to conventional anaesthetics however due to certain unusual reasons this it cannot be proposed as a first-line agent. Keywords: Tramadol hydrochloride Local Anesthesia Extraction.

Multicomponent coating in purulent wound healing: A randomised controlled experimental study

Background. Purulent wound healing is a pressing surgical challenge relevant in 30−35% of patient cases. To the more, wound infectious agents elaborate resistance to available drugs warranting the development of new drug combinations exserting a multidirectional effect on the wound process.Objective. Using a purulent wound model to experimentally evaluate the efficiency of a new multicomponent wound coating comprised of polyethylene oxide and carboxymethylcellulose sodium-immobilised dioxidine, methyluracil, metronidazole and lidocaine hydrochloride in comparison with a legal approved wound coating drug preparation of beeswax and propolis-based dioxidine ointment.Methods. The antimicrobial activity range (disk-diffusion method) and local anaesthetic effect (Rainier’s method) of the developed wound coating have been assessed. The healing process was studied in a purulent wound model with 72 Wistar rats divided between two equal groups. The following methods were applied: visual wound inspection (wound cleansing time, absence of wound-surrounding tissue oedema, granulation and epithelisation), planimetric parameter estimation (wound area, healing rate, wound area reduction ratio), wound contamination and pH measurement, wound section cell morphometry (granulocyte, macrophage, lymphocyte and fibroblast counts, cell index estimation). Daily dressings were applied for 15 days.Results. The developed wound coating exhibited high efficiency against Gram-positive and Gram-negative bacteria in the zone of inhibition tests. Its local anaesthetic effect was significantly superior to the approved drug by the duration of action. The wound area reduction was 94.2 (93.7; 94.8)% in the experimental group and 86 (84.2; 88.8)% in the control (differences statistically significant) already on day 10. A maximal healing rate in both groups was registered in phase 1 of the wound process being 1.4 times higher in experiment compared to the control. The wound contamination was significantly lower in experiment vs. control on day 8 (p = 0.0075). Wound pH negatively correlated with the fibroblast count and positively — with the contamination level.Conclusion. The study demonstrates high efficiency of the developed wound coating against infectious agents and its positive healing impact via reducing phase 1 and stimulating proliferation in phase 2 of the wound process.

Cholesterol Sequestration by Xenon Leads to Lipid Raft Destabilization

Combined coarse-grained (CG) and atomistic molecular dynamics (MD) simulations were performed to study the interactions of xenon with model lipid rafts consisting of 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC), 1,2-dilauroyl-sn-glycero-3-phosphocholine (DLPC) and cholesterol (Chol). At a concentration of 2 Xe/lipid we observed an unexpected result: Spontaneous nucleation of Xe nanoclusters which then rapidly plunged into the bilayer. In this process Chol, essential for raft stabilization, was pulled out from the raft into the hydrophobic zone. When concentration was further increased (3 Xe/lipid), the clusters disrupted both the membrane and raft. We computed the radial distribution functions, pair-wise potentials, second virial coefficients and Schlit-ter entropy to scrutinize the nature of the interactions. Our findings suggest that the well-known anaesthetic effect of Xe could be mediated by sequestration of Chol, which, in turn, compromises the stability of rafts where specialized proteins needed to produce the nervous signal are anchored.

Efficacy of Aqueous Crude Fruit Extract of Desert Date (Balanites aegyptiaca) in Anaesthetization of African Catfish (Clarias gariepinus) Fingerlings

Chemicals have been used to anaesthetize fish but due to their hazardous effects on the environment, fish and humans environmentally friendly plant anaesthetics are being sought. Fruit of Desert date (Balanites aegyptiaca) is nontoxic to humans but has been reported to have anaesthetic potentials on fish. This study investigates anaesthetic effect of aqueous crude fruit extract of B. aegyptiaca on African catfish (Clarias gariepinus) fingerlings. Phytochemical and proximate compositions of the fruit were screened. Exactly 120 C. gariepinus fingerlings (mean weight 32.13±2.43g and mean total length 23.88±2.11cm) were used for the experiment. A total of 10 fingerlings were exposed to each of 2.00, 2.50, 3.00, 3.50 and 4.00g/L concentrations of aqueous crude fruit extract of B. aegyptiaca and a control in 6 plastic tanks (45x28x25cm) filled with 10L of tap water. Setup was arranged in randomized block design and replicated. Temperature, dissolved oxygen, pH, free carbon dioxide and total alkalinity were monitored. Results revealed long mean induction (25.05±3.35 min) and recovery (108.35±2.45 min) times with resultant mortalities (40%) of C. gariepinus fingerlings. significant correlation (P<0.01) exists between concentration of the fruit extract and induction time as well as between fruit extract concentrations and survival of fingerlings. Aqueous crude fruit extract of B. aegyptiaca is, therefore, not effective for anaesthetization of C. gariepinus fingerlings hence should be avoided. Keywords: Anaesthetic, Balanites aegyptiaca, Clarias gariepinus

Anaesthetic effects of alfaxalone administered intraperitoneally alone or combined with dexmedetomidine and fentanyl in the rat

Alfaxalone is a neuroactive steroid used as a general anaesthetic in several species including dogs, cats, rabbits and ferrets. It has a wide margin of safety and a similar anaesthetic profile to propofol. To increase its aqueous solubility, a new formulation with cyclodextrins has been marketed recently. The objective of this study was to evaluate the anaesthetic effect of several doses of alfaxalone alone, considering differences between sexes, and alfaxalone combined with dexmedetomidine and fentanyl in the rat administered by the intraperitoneal route. A total of 40 Sprague Dawley rats, involved in three studies, were used. Firstly, 25, 35 and 45 mg kg–1 of alfaxalone alone were tested. In a second study, alfaxalone (25 mg kg–1, females; 75 mg kg–1, males) was combined with dexmedetomidine (0.05 mg kg–1). Finally, alfaxalone (20 mg kg–1, females; 60 mg kg–1, males) was combined with dexmedetomidine (0.05 mg kg–1) and fentanyl (0.1 mg kg–1). Times of onset and duration of anaesthesia, and analgesia, deemed as losing of withdrawal pedal reflex, were recorded. Alfaxalone alone produced a 2 - to 3-fold longer time of anaesthesia in females, although surgical anaesthesia was not achieved in either sex. The addition of dexmedetomidine and fentanyl to alfaxalone produced a similar time of analgesia as well as increased time of anaesthesia in both sexes. In conclusion, alfaxalone produces light anaesthesia in rats, and males required a higher dose. The combination with other sedatives or analgesics, such as dexmedetomidine or fentanyl, allows a more prolonged anaesthesia with analgesic effects, potentially suitable for invasive procedures.