scholarly journals Efficacy of Aqueous Crude Fruit Extract of Desert Date (Balanites aegyptiaca) in Anaesthetization of African Catfish (Clarias gariepinus) Fingerlings

2020 ◽  
Vol 10 (2) ◽  
pp. 45-52
Author(s):  
Idi Audu Wakawa ◽  
Abubakar Chiroma
Keyword(s):  
Tap Water ◽  
Block Design ◽  
Clarias Gariepinus ◽  
Total Alkalinity ◽  
African Catfish ◽  
Fruit Extract ◽  
Balanites Aegyptiaca ◽  
Randomized Block Design ◽  
Free Carbon Dioxide ◽  
Anaesthetic Effect

Chemicals have been used to anaesthetize fish but due to their hazardous effects on the environment, fish and humans environmentally friendly plant anaesthetics are being sought. Fruit of Desert date (Balanites aegyptiaca) is nontoxic to humans but has been reported to have anaesthetic potentials on fish. This study investigates anaesthetic effect of aqueous crude fruit extract of B. aegyptiaca on African catfish (Clarias gariepinus) fingerlings. Phytochemical and proximate compositions of the fruit were screened. Exactly 120 C. gariepinus fingerlings (mean weight 32.13±2.43g and mean total length 23.88±2.11cm) were used for the experiment. A total of 10 fingerlings were exposed to each of 2.00, 2.50, 3.00, 3.50 and 4.00g/L concentrations of aqueous crude fruit extract of B. aegyptiaca and a control in 6 plastic tanks (45x28x25cm) filled with 10L of tap water. Setup was arranged in randomized block design and replicated. Temperature, dissolved oxygen, pH, free carbon dioxide and total alkalinity were monitored. Results revealed long mean induction (25.05±3.35 min) and recovery (108.35±2.45 min) times with resultant mortalities (40%) of C. gariepinus fingerlings. significant correlation (P<0.01) exists between concentration of the fruit extract and induction time as well as between fruit extract concentrations and survival of fingerlings. Aqueous crude fruit extract of B. aegyptiaca is, therefore, not effective for anaesthetization of C. gariepinus fingerlings hence should be avoided. Keywords: Anaesthetic, Balanites aegyptiaca, Clarias gariepinus

scholarly journals Toxicological Impact of Leachates from Lemna Refuse Dump Site on African Catfish (Clarias gariepinus, Burchell 1882) Fingerlings Under Laboratory Condition

2021 ◽  
pp. 23-37
Author(s):  
Udeme Uyom Udofia ◽  
Finian Tobias Okoro ◽  
Akaninyene Paul Joseph
Keyword(s):  
Block Design ◽  
Clarias Gariepinus ◽  
Aquatic Systems ◽  
Control Group ◽  
African Catfish ◽  
Dump Site ◽  
Toxicological Effects ◽  
Lc50 Value ◽  
Randomized Block Design ◽  
Set Up

Aim: The study investigated the toxicological effects of Calabar municipal dumpsite leachate on the fingerlings of Clarias gariepinus. Experimental Design: The study was set-up in a 6 × 2 Complete Randomized Block Design. Methodology: During the studt 120 C. gariepinus fingerlings were used. Each group was made up of 10 fingerlings. The fingerlings were exposed to 0% (control group), 15%, 20%, 25%, 30% and 35% of leachate in duplicate. Histopathology was also carried-out on the gills and liver of the fingerlings of each exposure group. Results: The temperature, pH, conductivity, and BOD increased with increasing leachate concentration, while the DO decreased with concentration. 5.17 ± 0.66 cm and 1.09 ± 0.40 g mean length and weight respectively of fingerlings were determined. Mortality depended on concentration. The 96 hours LC50 value with 95% confidence limit of C. gariepinus fingerlings exposed to leachate was 22.5% ± 0.89, and was significant with a determination coefficient (r2) of 0.93 at P<0.05. Leachates altered the orientation of gills and liver of the fingerlings. Conclusion and Recommendations: The low LC50 value of fingerlings exposed to different concentrations of leachate indicates a high toxicity of the leachate and altered the gills and liver of fingerlings. To this end, we recommended dumpsites are sited at a distance far from water bodies and areas inhabited by human, to mitigate the leeching of leachate into nearby aquatic systems. Law against indiscriminate discharge of waste into drainage channels and any aquatic system should be enforced by Government, to prevent mortality of biological organisms, extinction of species, contamination of organisms in nearby aquatic systems, changes in the physical properties, and health risk to humans that depends on resources from these aquatic systems for food.

scholarly journals Effect of starter sources and old fermentation on making nata de whey towards chemical quality

2020 ◽  
Vol 142 ◽  
pp. 04001
Author(s):  
Asmaul Khusna ◽  
Anis Prastujati ◽  
Shinta Setiadevi ◽  
Mustofa Hilmi
Keyword(s):  
Reducing Sugars ◽  
Block Design ◽  
Reducing Sugar ◽  
Cellulose Content ◽  
Chemical Quality ◽  
Fruit Extract ◽  
Fermentation Time ◽  
Randomized Block Design ◽  
Pineapple Fruit ◽  
The Difference

Whey cheese is a by-product of traditional or modern cheese-making processes. The yield of each cheesemaking reaches around 83% of the volume of milk used.whey can be processed into nata de whey by adding Acetobacterxylinum. The purpose of this study were to determine the effect of fermentation duration and differences in starter sources on the chemical quality produced by nata de whey. All samples were analyzed for fat, protein, cellulose and reducing sugars. this use study of two factorial randomized block design. The first factor is the difference in the starter (commercial and pineapple fruit extract) and the second factor is the length of fermentation with three replications. The results showed that the effect of fermentation time and the difference in starter sources on nata de whey had a very significant impact (P <0.01) on the calculation of fat content in the nata the whey. The duration of fermentation and differences in the source of the stater on nata de whey had no significant effect (P> 0.05) on the content of protein, reducing sugar and cellulose content produced.

scholarly journals GROWTH OF AFRICAN CATFISH (Clarias gariepinus) JUVENILES FED GRADED LEVELS OF ROASTED RICE CHAFF AS ENERGY SOURCE

2020 ◽  
Vol 4 (2) ◽  
pp. 386-391
Author(s):  
Ahmed Salihu Dan-kishiya ◽  
C. C. Ekpenwa
Keyword(s):  
Energy Source ◽  
Body Weight ◽  
Low Cost ◽  
Block Design ◽  
Clarias Gariepinus ◽  
African Catfish ◽  
Fish Feed ◽  
Feed Conversion ◽  
Crude Fibre ◽  
Crude Lipid

The high cost of Maize as energy source in fish feed has necessitated the need to search for low cost and locally available indigenous sources. A six months feeding trial was conducted to examine the effects of replacing maize with roasted rice chaff as a dietary energy source on the growth of juveniles of Clarias gariepinus using Completely Randomized block Design with five different treatments. The fishes were subjected to five different inclusion levels of roasted rice chaff meal (0%, 25%, 50%, 75% and 100%). The trial was conducted in a 50litres plastic bowl with replicates and feeding was done initially at 3% body weight, but the quantity of feeds was adjusted based on the new weight gain. The measurements of the fishes were carried out biweekly according to standard methods and procedures. The Roasted rice chaff meal (RRCM) contains 5.93% crude protein, 14.53% crude fibre, 0.92% moisture content, while Ash content, crude lipid and carbohydrates had 19.98%, 3.28% and 55.36% respectively. Fishes fed 100% RRCM recorded the best growth performance in body weight (435.10 ± 20.60gm), total length (45.30 ± 0.70cm), Specific growth rate (1.44 ± 0.01), Feed Conversion Ratio (2.50 ± 0.04) and Feed Intake (92.00 ± 3.40). While the least growth ware observed in 0% RRCM. However, all the physico-chemical parameters measured are within the recommended range for fish culture. Therefore, the result obtained shows that roasted rice chaff can replace maize as a source of energy in Clarias gariepinus.juveniles diets without any negative effect.

scholarly journals PENGGUNAAN INSEKTISIDA BOTANIS UNTUK MENGENDALIKAN HAMA PADA TANAMAN TOMAT

EUGENIA ◽  
2013 ◽  
Vol 19 (2) ◽  
Author(s):  
C. S. Rante ◽  
D. T. Sembel ◽  
E. R.M. Meray ◽  
M. M. Ratulangi ◽  
M. F. Dien ◽  
...  
Keyword(s):  
Block Design ◽  
Control Treatment ◽  
Root Extract ◽  
Botanical Insecticide ◽  
Water Control ◽  
Fruit Extract ◽  
Tomato Plants ◽  
Randomized Block Design ◽  
Plant Pest ◽  
The Village

ABSTRACT   The experiment was conducted in the village of Touure, Tompaso District, Minahasa regency since April to August 2013. Designed studies using randomized block design with 3 treatments, namely fruit extract Bitung (treatment A), tuba root extract (treatment B) and water (control treatment), each treatment was repeated 3 times. Results showed that that the extract of Barringtonia asiatica and Derris elliptica has a botanical insecticide to control the properties of N. tenuis. Applications B. asiatica extract and D. elliptica were not significantly different, but both are significantly different from controls. In addition to N. tenuis found several Plant Pest Organisms (OPT) that attack tomato plants including pest Liriomyza sp., leafhoppers tomatoes., black stem base diseases, leaf spot diseases and viral diseases. Key words : Botanic Insecticide, Tomato pest control ABSTRAK   Penelitian dilaksanakan di Desa Toure, Kecamatan Tompaso, Kabupaten Minahasa sejak bulan April sampai dengan Agustus 2013.  Penelitian dirancang menggunakan Rancangan Acak Kelompok dengan 3 perlakuan yaitu ekstrak buah bitung (perlakuan A), ekstrak akar tuba (perlakuan B) dan air (perlakuan kontrol) yang masing-masing perlakuan diulang sebanyak 3 kali. Hasil penelitian menunjukkan bahwa bahwa ekstrak Barringtonia asiatica dan Derris elliptica memiliki sifat insektisida botanis untuk mengendalikan N. tenuis. Aplikasi ekstrak B. asiatica dan D. elliptica tidak berbeda nyata, namun keduanya berbeda nyata dengan kontrol.  Selain N. tenuis ditemukan juga beberapa Organisme Pengganggu Tanaman (OPT) yang menyerang tanaman tomat diantaranya hama Liriomyza sp., wereng tomat., penyakit pangkal batang hitam, penyakit bercak daun dan penyakit virus. Kata kunci : Insektisida botanis, pengendalian hama tanaman tomat

Titanium dioxide nanoparticles-induced cytogenotoxicity and alterations in haematological indices of Clarias gariepinus (Burchell, 1822)

2020 ◽  
Vol 36 (10) ◽  
pp. 807-815
Author(s):  
Opeoluwa M Ogunsuyi ◽  
Elizabeth O Adegoye ◽  
Olusegun I Ogunsuyi ◽  
Okunola A Alabi ◽  
Chibuisi G Alimba ◽  
...  
Keyword(s):  
Titanium Dioxide ◽  
Tap Water ◽  
Clarias Gariepinus ◽  
Haemoglobin Concentration ◽  
Exposure Period ◽  
Negative Control ◽  
African Catfish ◽  
Mean Cell Volume ◽  
Corpuscular Haemoglobin ◽  
Mean Corpuscular Haemoglobin

The application of titanium dioxide (TiO2) nanoparticles (NPs) in the manufacturing of consumer products has increased tremendously and with the potential to induce deleterious effects on aquatic biota. There have been reports on metal oxide NP toxicity in aquatic organisms, however, information on cytotoxicity and genotoxicity of TiO2 NPs on the African catfish, Clarias gariepinus, is scarce. In this study, we investigated the genotoxicity and haematotoxicity of TiO2 NPs in C. gariepinus using the micronucleus (MN) assay and haematological analysis, respectively. Juvenile C. gariepinus were exposed to 6.25, 12.5, 25.0, 50.0 and 100.0 mg L−1 concentrations of TiO2 NPs for 7 and 28 days. Benzene (0.05 mL L−1) and dechlorinated tap water were used as positive and negative controls, respectively. Data of the MN showed a significant ( p < 0.05) concentration-dependent increase in the frequency of MN at both exposure periods in comparison to negative control. Red blood cells, haematocrit, platelets and heterophils significantly reduced with an increased mean corpuscular haemoglobin concentration and lymphocytes at the 7-day exposure period, while in the 28-day exposure period, mean cell volume, mean corpuscular haemoglobin and lymphocytes had a significant increase in comparison with the negative control. This study indicates that TiO2 NPs induced cytogenetic and haematological alterations in C. gariepinus and is of relevance in biodiversity and aquatic health management.

scholarly journals Post feeding protection from Bilimbi to Motile Aeromonas Septicemia disease in African Catfish

DEPIK ◽  
2021 ◽  
Vol 10 (2) ◽  
pp. 120-124
Author(s):  
Sefti Heza Dwinanti ◽  
Gusti Setiawan ◽  
Ade Dewi Sasanti ◽  
Madyasta Anggana Rarassari
Keyword(s):  
Water Quality ◽  
Feed Efficiency ◽  
Block Design ◽  
Control Fish ◽  
African Catfish ◽  
Factor B ◽  
Post Feeding ◽  
Randomized Block Design ◽  
Fish Survival ◽  
Protection Period

Bilimbi (Averrhoa bilimbi) is plant medicinal used to control fish diseases both prevention and treatment. This study aims to determine the protection period of bilimbi-fed which different duration in catfish to enhance catfish (Clarias sp.) immunity in order to protect fish from Aeromonas hydrophila infection. The Randomized Block Design Factorial (RBDF) with 2 factors was used in this experimental. The first factor (A) was the period of feeding containing bilimbi juice consisting of 3 treatments, namely 0 days (P0), 7 days (P1), and 14 days (P2). The second factor (B) was the protection period after the administration of bilimbi juice to the infection of A. hydrophila bacteria consisting of 3 treatment, 5 days (T5), 10 days (T10), and (T15). This research was conducted at the Laboratory of Aquaculture Experiment Pond, Faculty of Agriculture, Sriwijaya University in October-December 2018. The parameters observed included prevalence of infection, growth, survival, feed efficiency and water quality. The result revealed that by giving 300 mL.kg-1 of bilimbi juice with different periods significantly affected the period of fish protection for A. hydrophila infection. The best prevalence value was 3,33% (P2T5), and the best survival of the fish before infection was 94,17% (P2), while the best fish survival post infection was 100% (P2T5), the best feed efficiency value was 56,47% (P2), water quality under experiment were 26-29,70C for temperature, pH 6.8-7.7, dissolved oxygen 4.35-5.37 mg/L and ammonia 0.09-0.21 mg/L.Keywords:Averrhoa bilimbiAeromonas hydrophilaEthnomedicinal plantFish immunityMotile Aeromonas Septicemia

scholarly journals Pengendalian Layu Fusarium Menggunakan Mikrobe Antagonis dan Tanaman Resisten pada Lili

2011 ◽  
Vol 21 (4) ◽  
pp. 338
Author(s):  
Wakiah Nuryani ◽  
Evi Silfia Yusuf ◽  
Hanudin ◽  
Ika Djatnika ◽  
Budi Marwoto
Keyword(s):  
Tap Water ◽  
Human Life ◽  
Block Design ◽  
Economic Value ◽  
Wilt Disease ◽  
Control Measures ◽  
Lower Percentage ◽  
Randomized Block Design ◽  
Trichoderma Sp ◽  
Bulb Rot

Lili merupakan tanaman hias penting dan bernilai ekonomi tinggi. Budidaya lili di Indonesia menghadapi kendala utama yaitu penyakit layu Fusarium. Aplikasi bahan kimia sintetik untuk mengendalikan penyakit ini berdampak negatif terhadap kerusakan lingkungan. Aplikasi organisme antagonis dan kultivar resisten merupakan alternatif pengendalian penyakit ramah lingkungan, berdampak positif terhadap kelestariannya, dan meningkatkan produktivitas lili. Penelitian bertujuan untuk mendapatkan informasi mikrobe antagonis, mikrobe nonpatogenik, dan klon lili yang dapat menekan penyakit  layu Fusarium. Penelitian dilakukan di Laboratorium Biokontrol dan Rumah Sere, Balai Penelitian Tanaman Hias pada bulan Januari sampai dengan Desember 2007. Tata letak percobaan disusun berdasarkan rancangan acak kelompok pola faktorial denga tiga ulangan. Faktor pertama ialah klon lili yang terdiri dari klon No.1, No 2, No.3 (tahan), dan No.4 (rentan). Faktor kedua ialah perlakuan mikrobe antagonis (1) Gliocladium sp. 107 spora/ml, (2). Trichoderma sp. 107 sel/ml, (3) Fusarium a virulen 107 spora/ml dan, (4) kontrol (tanpa mikrobe antagonis). Hasil percobaan menunjukkan bahwa penggunaan mikrobe antagonis Gliocladium sp. dan klon resisten paling efektif mengendalikan penyakit busuk umbi Fusarium pada lili. Hal ini dibuktikan dari persentase tanaman layu pada perlakuan aplikasi mikrobe dan penggunaan tanaman resistant dibandingkan dengan tanpa perlakuan. Penanaman tanaman resisten diikuti dengan aplikasi Gliocladium paling efektif menekan layu Fusarium dibanding perlakuan lainnya.<br /><br /><br /><br />Lilium is one of  the most important and the heigest economic value of cut flower in Indonesia. Cultivation of Lilium faces major constrain, that is wilt disease caused by Fusarium. Application of synthetic chemicals to control the diseases is not recommended, because its impact is dangerous for environment and human life. Therefore other control measures that are move environmentally friendly and more effective/efficient have to applied. The use of antagonistic microbes and resistance varieties are the most promising control measures to be recommended in the field. The study was aimed to find out of antagonist and nonpathogenic microbes and plant resistant that were effective to control Fusarium bulb rot on lili.  The experiment was conducted at Laboratory and Glasshouse of Indonesian Ornamental Crops Research Institute (1,100 m asl.) on January to December 2007. Factorial experiment was arranged in a randomized block design with three replications. The first factor was Lilium clones i.e. No.1, No. 2, No. 3 (resistant), and No. 4 (susceptible clone as control). The second factor was antagonism microbes i.e. (1) Gliocladium sp. 107 spora/ml, (2). 107 Trichoderma sp. cel/ml, (3) Fusarium nonpathogenic 107 spora/ml, and (4) control (tap water without antagonist microbe). The results indicated that the use of Gliocladium sp. and resistant clone of Lilium i.e. clone No.3 was effective to control Fusarium bulb rot of Lilium. This proven from lower percentage of disease occurance on the treatment of antagonistics microbes and resistance varieties compared both to those of without treatment (control). The use of resistance plant followed by application of Gliocladium was known to be most effective to control fusarial wilt disease compared to the other treatment combinations.   <br /><br />

scholarly journals Haematological Response of African Catfish (Clarias gariepinus Burchell 1822) Fingerlings Exposed to Different Concentrations of Tobacco (Nicotiana tobaccum) Leaf Dust

ISRN Zoology ◽  
2013 ◽  
Vol 2013 ◽  
pp. 1-7 ◽  
Author(s):  
Safina M. Musa ◽  
Christopher Mulanda Aura ◽  
Erick Ochieng Ogello ◽  
Reuben Omondi ◽  
Harrison Charo-Karisa ◽  
...  
Keyword(s):  
Clarias Gariepinus ◽  
Survival Rates ◽  
Exposure Period ◽  
Quality Parameters ◽  
Total Alkalinity ◽  
African Catfish ◽  
Fish Ponds ◽  
Tobacco Leaf ◽  
Baseline Information ◽  
Safe Limits

The present study set out to investigate the haematological effects of tobacco leaf dust on African catfish, Clarias gariepinus, fingerlings, with a mean weight of 3.01 ± 1.25 g using “static renewal bioassay system” during a 120-hour bioassay exposure period. Water quality parameters such as pH and dissolved oxygen significantly decreased while total alkalinity and conductivity increased significantly in the exposed media, compared to the control test. Leucocytes counts increased significantly while erythrocytes counts decreased significantly with increasing concentration of tobacco dust. Packed cell volume significantly reduced with increase in the concentration of tobacco dust. Haematological examination showed that there was destruction of the erythrocytes production, and the concentration of haemoglobin was much lower in the exposed fish compared to the control depicting an anaemic condition. The results could provide baseline information for the safe limits of using tobacco leaf dust in fish ponds; hence 1.56 g L−1 concentration of tobacco leaf dust was recommended for pond preparation for Clarias gariepinus fingerling stocking. For better survival rates, the fish should only be introduced in the pond after three days of tobacco application.

scholarly journals Penggunaan Ekstrak Buah Hutung (Barringtonia Asiatika) Untuk Mengendalikan Hama Maruca Testulalis Penggerek Polong Pada Kacang Panjang

Agrologia ◽  
2017 ◽  
Vol 6 (2) ◽  
Author(s):  
Riski Siahaya ◽  
Victor George Siahaya ◽  
Marthin Kalay ◽  
Abraham Talahaturuson
Keyword(s):  
Crop Yields ◽  
Block Design ◽  
Control Plant ◽  
Maruca Testulalis ◽  
Time Interval ◽  
Application Time ◽  
Fruit Extract ◽  
Synthetic Chemicals ◽  
Randomized Block Design ◽  
Plant Pest

The use of synthetic chemicals to control plant pest should to be limited by utilizing natural products so that environmental contamination can be reduced and crop yields are safe to eat. The aim of this research is to get the effective concentration and application time of hutung fruit (Barringtonia asiatika) extract to control Maruca testulalis pod borer on long bean. The experiments design was complety randomized block design with treatments of  5% extract of hutung fruit with interval of time of two days application, 5% extract of nutmeg with time interval of three days application, 10% extract of nutmeg with time interval of two days application, 10% fruit extract hutung with a three-day application time interval. Two control treatments were 0.1% Decis insecticide with a three-day application time interval three replications and without any treatment. The result showed that 10% hutung fruit extract with two days application time interval was the best treatment and could decrease the intensity of Maruca testulalis pest borer attack on long bean up to 39,14%.

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