Qualitative Estimation of Seed of Butea monosperma Lam. by using Chromatography Technique

2021 ◽  
pp. 5466-5470
Author(s):  
Md. Rageeb Md. Usman ◽  
Shaikh Salman Shaikh Babu
Keyword(s):  
Gallic Acid ◽  
Chemical Constituent ◽  
Phytochemical Study ◽  
Butea Monosperma ◽  
Hplc System ◽  
Active Chemical ◽  
Qualitative Estimation

The objective of present studies deals with the Qualitative estimation of seed of Butea monosperma Lam. By using Chromatography Technique. The phytochemical study of different extract of seed of Butea monosperma Lam. Were observed various active chemical constituent like phytosterol, flavonoid, saponin and sterol etc. Qualitative estimation of Gallic acid, Rutin and Quarcetin was carried out by HPTLC and HPLC system.

scholarly journals Production of bergenin, an active chemical constituent in the callus of Bergenia ciliata (Haw.) Sternb.

2012 ◽  
Vol 8 ◽  
pp. 40-44 ◽  
Author(s):  
Umesh Krishna Shrestha ◽  
Bijaya Pant
Keyword(s):  
Leaf Explants ◽  
Chemical Constituent ◽  
Wild Plants ◽  
Plant Science ◽  
Naphthalene Acetic Acid ◽  
Active Chemical ◽  
In The Wild ◽  
The Media ◽  
Benzyl Amino Purine

In vitro culture of Bergenia ciliata (Haw.) Sternb. was carried out for the examination of bergenin content. Leaf explants were cultured in MS (Murashige and Skoog) basal media supplemented with or without phytohormones. The hormonal series maintained were in the range of 0-2 mg l-1 for BAP (6-benzyl amino purine) and 0-1.5 mg l-1 for NAA (α-naphthalene acetic acid). Bergenin content of in vitro grown tissues of B. ciliata was compared with that of wild plants collected from three different localities of Nepal. The best growth of callus and plantlets occurred in the media containing BAP 1.0 mg l-1 + NAA 1.0 mg l-1 and BAP 1.5 mg l-1 + NAA 1.0 mg l-1. Production of bergenin was high in the media supplemented with 1.0 mg l-1 BAP + 1.5 mg l-1 NAA (3.40 μg g-1) and 2.0 mg l-1 BAP + 1.5 mg l-1 NAA (3.05 μg g-1) under experimental condition. The bergenin content in the wild plants collected from Langtang, Jumla and Godawari was found to be 4.28 μg g-1, 4.53 μg g-1 and 3.64 μg g-1 respectively. This study shows that the in vitro cultured callus of B. ciliata is capable of synthesizing bergenin in quantity comparable to that of the wild plant.doi: http://dx.doi.org/10.3126/botor.v8i0.5557 Botanica Orientalis – Journal of Plant Science (2011) 8: 40-44

scholarly journals ANTIMICROBIAL ACTIVITY AND PHYTOCHEMICAL STUDY OF PLANT PARTS OF BUTEA MONOSPERMA

2019 ◽  
Vol 9 (4-A) ◽  
pp. 344-348
Author(s):  
KRUTIBEN MUKESHBHAI DAVE ◽  
Parth Pankaj Darji ◽  
Fenie Ritesh Gandhi
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Antibacterial Activity ◽  
Antimicrobial Agents ◽  
Herbal Medicines ◽  
Host Cells ◽  
Phytochemical Study ◽  
Chemical Test ◽  
Medicinal Uses ◽  
Butea Monosperma

ABSTRACT: Since ancient times, plants have been model source of medicines as they are a reservoir of chemical agents with therapeutic properties. The general population is increasingly using herbal medicines as dietary supplements to relieve and treat many different human disorders. All parts of the plant, from root to fruit, consisting of a multitude of secondary metabolites which impart an unprecedented variety of medicinal uses to the plant. Studies have shown the presence of different phytochemical constituents in botanical sample responsible for the antimicrobial activity. These antimicrobial agents should be beneficial to host cells and toxic to pathogenic microbes. Hence, the antibacterial activity was examined from the leaf and flower of Butea monosperma. Sample was collected and its crude extract was obtained by using methanol, acetone and water as the extraction solvent. These extract were tested against some pathogenic microorganisms like Staphylococcus aureus , Bacillus cereusa and B. subtilis. The extract of Butea monosperma showed antibacterial activity against Staphylococcus aureus , Bacillus cereusa and B. subtilis. Bio-chemical test for the presence of phytochemicals have shown positive result and these phytochemicals have ability to fight against microorganisms or inhibit the growth of microorganisms. This approach will be an advanced step in the discovery of some herbal drugs. These plant extracts which was proven to be potentially effective can be used as a natural alternative to the chemical preservatives. It could be an ideal way to avoid health hazards that may occur due to chemical antimicrobial agents.

scholarly journals Phytochemical Screening and Evaluation of Antioxidant Properties and Antimicrobial Activity against Xanthomonas axonopodis of Euphorbia tirucalli Extracts in Binh Thuan Province, Vietnam

Molecules ◽  
2021 ◽  
Vol 26 (4) ◽  
pp. 941
Author(s):  
Nguyen Thị My Le ◽  
Dang Xuan Cuong ◽  
Pham Van Thinh ◽  
Truong Ngoc Minh ◽  
Tran Dinh Manh ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Ethyl Acetate ◽  
Gallic Acid ◽  
Antioxidant Properties ◽  
Ethyl Acetate Fraction ◽  
Plant Diseases ◽  
Phytochemical Screening ◽  
Phytochemical Study ◽  
Xanthomonas Axonopodis ◽  
Euphorbia Tirucalli

Euphorbia tirucalli is a medicine plant possessing many bioactive properties. This paper focused on phytochemical screening (alkaloid, flavonoid, saponin, tannin, and anthraquinone), quantification of polyphenol and flavonoids, and activating evaluation of antioxidants and antimicrobial properties against Xanthomonas axonopodis of different extracts from Euphorbia tirucalli grown in Binh Thuan, Vietnam. The best activity fraction was used for purification and determining bioactive ingredients. The results showed that the phytochemical study revealed the presence of alkaloids, flavonoids, tannins, and terpenoids in the ethyl acetate fraction. Saponin and anthraquinone did not present in all extracts. The content of polyphenol and flavonoid of Euphorbia tirucalli stem was in the range of 16.65–106.32 mg EqAG/g and 97.97–450.83 μg QE/g. The ethyl acetate fraction showed higher amounts of polyphenol and flavonoids and antimicrobial activity against X. axonopodis than other fractions. The antioxidant (SC50) activity of Euphorbia tirucalli stem was in the range of 12.91 ± 0.70 and 528.33 ± 25.15 μg/mL. At concentrations of 5.0 and 7.5 mg/mL, the diameter of inhibition of the ethyl acetate fraction was 14.33 ± 0.76 mm and 17.87 ± 0.57 mm, respectively. The MIC (minimum inhibitory concentration) was 0.156 mg/mL. Scopoletin, gallic acid, and piperic acid got MICs corresponding to 78, 312, and 312 μg/mL, respectively. Scopoletin, gallic acid, and piperic acid were found in the ethyl acetate fraction of Euphorbia tirucalli and exhibited the treatment of citrus bacteria canker and plant diseases.

scholarly journals The Potential Involvement of an ATP-Dependent Potassium Channel-Opening Mechanism in the Smooth Muscle Relaxant Properties of Tamarix dioica Roxb.

Biomolecules ◽  
2019 ◽  
Vol 9 (11) ◽  
pp. 722 ◽  
Author(s):  
Syeda Imtiaz ◽  
Ambreen Aleem ◽  
Fatima Saqib ◽  
Alexe Ormenisan ◽  
Andrea Elena Neculau ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Potassium Channel ◽  
Gallic Acid ◽  
Castor Oil ◽  
Katp Channel ◽  
Ex Vivo ◽  
Phytochemical Study ◽  
Channel Opening ◽  
Cardiovascular Systems

Background: Tamarix dioica is traditionally used to manage various disorders related to smooth muscle in the gastrointestinal, respiratory, and cardiovascular systems. This study was planned to establish a pharmacological basis for the uses of Tamarix dioica in certain medical conditions related to the digestive, respiratory, and cardiovascular systems, and to explore the underlying mechanisms. Methods: A phytochemical study was performed by preliminary methods, followed by HPLC-DAD and spectrometric methods. In vivo evaluation of a crude hydromethanolic extract of T. dioica (TdCr) was done with a castor-oil-provoked diarrheal model in rats to determine its antidiarrheal effect. Ex vivo experiments were done by using isolated tissues to determine the effects on smooth and cardiac muscles and explore the possible mechanisms. Results: TdCr tested positive for flavonoids, saponins, phenols, and tannins as methanolic solvable constituents in a preliminary study. The maximum quantity of gallic acid equivalent (GAE), phenolic, and quercetin equivalent (QE) flavonoid content found was 146 ± 0.001 μg GAE/mg extract and 36.17 ± 2.35 μg QE/mg extract. Quantification based on HPLC-DAD (reverse phase) exposed the presence of rutin at the highest concentration, followed by catechin, gallic acid, myricetin, kaempferol, and apigenin in TdCr. In vivo experiments showed the significant antidiarrheal effect of TdCr (100, 200, and 400 mg/kg) in the diarrheal (castor-oil-provoked) model. Ex vivo experiments revealed spasmolytic, bronchodilatory, and vasorelaxant activities as well as partial cardiac depressant activity, which may be potentiated by a potassium channel opener mechanism, similar to that of cromakalim. The potassium channel (KATP channel)-opening activity was further confirmed by repeating the experiments in glibenclamide-pretreated tissues. Conclusions: In vivo and ex vivo studies of T. dioica provided evidence of the antidiarrheal, spasmolytic, bronchodilator, vasorelaxant, and partial cardiodepressant properties facilitated through the opening of the KATP channel.

scholarly journals Phytochemical Study and Antioxidant Activity of Annona muricata (Annonaceae) and Corchorus olitorius (Tiliaceae) Two Medicinal Plants from the Ivorian Pharmacopoeia Used in the Treatment of Diabetes

2021 ◽  
Vol 9 (1) ◽  
pp. 31-42
Author(s):  
Abba P. Obouayeba Obou C. Okou ◽  
Bognan J.A.A. Ackah Bi Tra L. Tra ◽  
Tanoh H. Kouakou Allico J.Djaman ◽  
Jean D. N guessan
Keyword(s):  
Antioxidant Activity ◽  
Medicinal Plants ◽  
Gallic Acid ◽  
Aqueous Extract ◽  
Corchorus Olitorius ◽  
Annona Muricata ◽  
Phytochemical Screening ◽  
Phytochemical Study ◽  
Ic50 Value ◽  
Treatment Of Diabetes

Annona muricata and Corchorus olitorius are two medicinal plants whose leaves are widely used in Ivory Coast both in nutrition and for the treatment of various pathologies because of their phytochemical compositions. The objective of this study was to determine the phytochemicals and to assess the antioxidant property of the leaves of these two plants. The phytochemical study was carried out using tests for the identification and quantification of phytoconstituents. As for the study of antioxidant activity, it was carried out by measuring the anti-free radical activity either by ABTS or by DPPH. Qualitative phytochemical screening revealed the presence of flavonoids, polyphenols, saponins, sterols, catechetical tannins and terpenes. The quantitative phytochemical screening of the extracts showed that the content of flavonoids (329.10 ± 2.51 mg Eq quercetin/Kg) and that of polyphenols (82.09 ± 0.13 mg Eq gallic acid/g) of Annona muricata are higher than the content of flavonoids (178.45 ± 1.09 mg Eq quercetin/Kg) and that of polyphenols (67.98 ± 5.22 mg Eq gallic acid/g) of Corchorus olitorius. The results of the antioxidant activity by measurement of the ABTS radical shows that the aqueous extract of Annona muricata exhibits the best activity with an IC50 value of 1.79 ± 0.15 μM, while the aqueous extract of Corchorus olitorius records an IC50 value of 2.64 ± 0.05 μM. Similar results are observed with the measurement of DPPH. The various metabolites demonstrated have various therapeutic properties which may be involved in the treatment of diabetes. This would confirm the therapeutic use reported by the populations. However, the evaluation of the antimalarial, antibacterial and anti-inflammatory activities as well as the toxicity test of the aqueous extract of these plants would be necessary.

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