EVALUATION OF ROMANIAN APPLE CULTIVARS IN LATVIA

Commercial apple growing in Latvia feels some lack of climate adapted modern cultivars. Development of fruits and trees is limited by active growth period (temperatures over 10 °C) of 135-150 days and active temperature sum of 1700-2100 °C. Winter hardiness also is essential, as temperatures below −30°C occur each 10 years, and winter thaws are frequent. Annual precipitation 692 mm favours development of fungal diseases. In 2016, a trial of four Romanian apple cultivars resistant to scab - ‘Aura’, ‘Ciprian’, ‘Jonaprim’ and ‘Romus 3’ was established in Latvia. Trees were grafted on dwarfing rootstock B.396 and planted as 1-year old whips in 3 replications with 2-3 trees in each replication, and trained as slender spindle. Commercial cultivars ‘Auksis’ (midseason) and ‘Ligol’ (late) were used as controls. Most trees of all cultivars in the 2nd year (2017) developed a well branched frame, from 37.5% trees of ‘Ciprian’ to 90% trees of ‘Jonaprim’. The first fruits were harvested in 2017, and commercially significant yield was achieved in 2018, except ‘Jonaprim’, which had the slowest yield increase. By the sum of yield in 2017-2021 ‘Aura’ and ‘Jonaprim’ surpassed commercial cultivar ’Auksis’, but were less productive than ‘Ligol’. Only slightly lower summary yield was obtained from ‘Ciprian’, while ‘Romus 3’ showed the lowest productivity and strong premature fruit drop. Very good taste panel results were obtained for ‘Aura’ and ‘Ciprian’, 7 to 8 points in average. Their fruits also kept well, to 5 months in common storage at 2 ±1oC. Fruits of the other two cultivars were too small and had mediocre or poor taste. The weather conditions in the trial years allowed to test susceptibility to spring frosts and drought. All cultivars showed good tree health after over-wintering. ‘Jonaprim’ and ‘Auksis’ in 2019 had the highest spring frost injury, 60-70% of fruits, while least injured were ‘Aura’ (21.5%) and ‘Ciprian’ (27.7%). Extreme drought in summer of 2021 led to low average fruit mass of all cultivars including the normally large fruited ‘Ligol’ (120-130 g), while in other years ‘Aura’ had fruits over 180 g, very attractive yellow with red stripes. Fruits of ‘Ciprian’ also had good size, but in some years too dark over-colour. ‘Aura’ and ‘Ciprian’ showed good adaptation to the climate of Latvia and can be recommended for wider trials at farms.

Skeletal and dental changes induced by the Flip-Lock Herbst appliance in the treatment of Angle’s class II division 1 malocclusion during active growth period: A preliminary study

Background. The Flip-Lock Herbst (TP Orthodontics Inc.) is a fixed functional appliance, a variant of the Herbst appliance, introduced by Miller. It is claimed to have better patient tolerance due to its increased freedom for the mandible’s lateral movements. There have been no studies on the flip lock Herbst till date. This study was undertaken to assess the efficiency of the Flip-Lock Herbst appliance in correcting Angle’s class II division 1 malocclusion. Methods. Eight subjects in their active growth period with class II division 1 malocclusion due to a retrognathic mandible were included in the study. Standardized lateral cephalometric radiographs were used to evaluate skeletal and dental changes with the SO analysis. Paired samples t-test was used to assess statistical significance. Results. Statistically significant increases in mandibular length (pg/OLp) and effective mandibular length (ar/OLp + pg/OLp) were observed. There was a significant maxillary restraining effect. Dental effects were significant and exhibited class II correction features except for the position of lower incisors within the mandible (ii/OLp - pg/OLp). Skeletal changes accounted for 61% and dental changes for 39% of the total treatment for molar correction. For overjet correction, skeletal changes contributed to 63% and dental changes to 37% of the total treatment. Conclusion. The Flip-Lock Herbst appliance was efficient in correcting Angle’s class II division 1 malocclusion due to a retrognathic mandible. Both skeletal and dental changes were evident, with the former predominating (60:40).

Long-term standardized forest phenology in Sweden: a climate change indicator

Because climate change alters patterns of vegetative growth, long-term phenological measurements and observations can provide important data for analyzing its impact. Phenological assessments are usually made as records of calendar dates when specific phase changes occur. Such assessments have benefits and are used in Citizen Science monitoring. However, these kinds of data often have low statistical precision when describing gradual changes. Frequent monitoring of the phenological traits of forest trees and berries as they undergo gradual change is needed to acquire good temporal resolution of transitions relative to other factors, such as susceptibility to frosts, insects, and fungi, and the use of berries as a food resource. Intensive weekly monitoring of the growth of apical and branch buds and the elongation of shoots and leaves on four tree species, and the abundance of flowers and berries of bilberry and lingonberry, has been performed in Sweden since 2006. Here, we present quantitative methods for interpolating such data, which detail the gradual changes between assessments in order to describe average rates of development and amount of interannual variation. Our analysis has shown the active growth period of trees to differ with latitude. We also observed a change in the timing of the maximum numbers of ripening berries and their successive decline. Data from tree phenology assessments can be used to recommend best forestry practice and to model tree growth, while berry data can be used to estimate when food resources for animals are most available.

Deoxynivalenol (DON) Accumulation and Nutrient Recovery in Black Soldier Fly Larvae (Hermetia illucens) Fed Wheat Infected with Fusarium spp.

Fusarium head blight (FHB) is one of the most significant causes of economic loss in cereal crops, resulting in a loss of $50–300 million for Canadian agriculture. The infected grain (containing Fusarium-damaged kernels (FDKs)) is often both lower in quality and kernel weight, and it may be unsuitable for human and animal consumption due to mycotoxin presence. However, it still contains a considerable amount of nutrients. A method to recover the nutrients without the mycotoxins should be beneficial for the agricultural economy. In this study, our objective was to examine recovery methods of the nutrients in relation to mycotoxin accumulation in the insect. The FDKs were fermented with Aspergillus oryzae and/or Lactobacillus plantarum (solid-state fermentation (SSF)). The SSF kernels were then provided to 50 young, black soldier fly larvae (BSFL) for 12 days. Weight gain, chemical composition, and mycotoxin bioaccumulation of BSFL and spent feed were evaluated. After 12 days of insect culture, the BSFL grew 5–6 times their initial weight. While the overall weights did not significantly vary, the proteins and lipids accumulated more in SSF FDK-fed insects. During the active growth period, the larval biomass contained deoxynivalenol (DON), a mycotoxin, at detectable levels; however, by day 12, when the larvae were in the pre-pupal stage, the amount of DON in the insect biomass was nearly negligible, i.e., BSFL did not accumulate DON. Thus, we conclude that the combination of BSFL and SSF can be employed to recover DON-free nutrients from FHB-infected grain to recover value from unmarketable grain.

Morphogenesis of age groups of Marandu palisade grass tillers deferred and fertilised with nitrogen

Nitrogen fertilisation allows to modulate the growth of forage canopy during the deferment period. We evaluated the morphogenic characteristics, during the initial and final phases of the deferment period, of young, mature and old tillers of Brachiaria brizantha cv. Marandu. fertilised with low and high doses of nitrogen (N). The design was completely randomised, in a subdivided plot scheme, with four replications. Leaf appearance rate (LAR) and leaf elongation rate (LER) were higher in young tillers than in old ones. At the beginning of the deferment period, the canopy under high N doses had higher LAR and LER values. At the end of the deferment period, we observed no difference between the doses of N for LAR. The stem elongation rate was higher at the beginning than at the end of the deferment period, while the leaf senescence rate (LSR) was higher at higher N doses when compared to lower doses. The highest LSR values and leaf lifespan were observed at the end of the deferment period. The greatest number of young tillers occurred at the beginning of the deferment period and with the highest N dose. In this sense, higher N doses, applied during the active growth period and before the deferment period, favour he maintenance of a larger number of young tillers, which have a greater growth potential.

Morphological Variations of Nutrient Foramina in Upper Limb Long Bones

The nutrient artery is the principal source of blood supply to a long bone and is particularly important during its active growth period in the embryo and foetus as well as during the early phase of ossification. The aim of the present study was to study the topographic anatomy and morphology of the nutrient foramina in human adult upper limb long bones. The study was performed on 40 upper limb long bones which include 40 humerii, 40 radii, 40 ulnae. The bones were obtained from department of anatomy Punjab institute of medical sciences, Jalandhar. The variations were found in number and location of nutrient foramen in different upper limb bones. In humerus double and triple foramina were found. In radius and ulna double foramina were observed at the maximum. Absence of nutrient foramen was observed in radius. The knowledge about these foramina is useful in surgical procedures.

L-Tryptophan Production by Auxotrophic and Analogue Resistant Mutants of Aureobacterium flavescens

A number of tyrosine plus phenylalanine double auxotrophic mutants were isolated by N-methyl-N-nitro-N-nitrosoguanidine (MNNG) treatment of a locally isolated strain of Aureobacterium flavescens of which 11A39 and 11A17 were selected on the basis of their tryptophan production in a mineral salt medium over other isolated mutant strains. The mutational block in the aromatic amino acid biosynthetic pathway of the selected double auxotrophs were determined. By controlling pH of the production medium to near neutrality, the active growth period could be extended up to 72 h and more tryptophan was accumulated compared to pH unregulated culture where the active growth ceased after 48 h. Further improvement of the tryptophan production has been achieved by stepwise isolation of a mutant strain resistant to the tryptophan analogues p-fluorotryptophan (FT) and 5-methyl tryptophan (MT) from the 11A39. Demand for L-tryptophan as food additive and therapeutic agent is increasing day by day throughout the World, particularly in the underdeveloped and developing countries like India. Still to date India depends on other countries for L-tryptophan. The aim of this work is to develop a potent high yielding, feed back insensitive mutant strain and optimization of its medium pH for maximum production of tryptophan.

Impact of harvesting and fire on Phragmites australis reed quality in Tembe Elephant Park, Maputaland

In Maputaland, South Africa, the common reed (Phragmites australis) is used extensively for hut building, fencing, craftwork and thatching. As a result of over-harvestingmost reed beds in communal areas have been degraded and are no longer producing reeds of the desired quality. At present the most productive reed beds are all found inconservation areas. The KwaMsomi area of the Muzi Swamp in the Tembe Elephant Park has been allocated to the Sibonisweni community for harvesting purposes. Thiscommunity has recently requested Ezemvelo KwaZulu-Natal Wildlife for additional areas for harvesting on the grounds that the current site was no longer yielding reeds of suitable quality. The main objective of this study was therefore to determine whether there was a decline in reed quality in the KwaMsomi harvested area. The results of thisstudy suggest that harvested areas contained more thin, short reeds than unharvested areas. Fire can be used to increase reed diameter in harvested areas, but will not significantly affect reed height. Ideally, reeds should only be harvested after the active growth period, when most of the nutrient reserves have been translocated to the rhizomes andthe buds are still dormant. To improve reed quality a three-year rotational harvesting programme should be implemented to allow the reeds to recover fully before being harvested again.

Availability of nitrogen after fertilizer applications to cereals

An experiment was conducted over seven sites in eastern England sown to winter cereals, whereby the soil mineral nitrogen (N) and N recovery in the crop were measured frequently during the active growth period. Plots received 100 kg/ha fertilizer N on one of three dates in spring (28 March, 11 April and 25 April) on each site in each of the three years (1994 to 1996), and these were compared with controls with no applied N. At one site, uncropped plots were included, and measurements of gaseous N emission, soil respiration, soil microbial biomass and root tissue N concentration were also made. The fertilizer applications boosted yields by at least 3 t/ha at all sites, but apparent recovery of fertilizer N varied throughout the previously known range of 45–85%. Soil type and timing of application had no effect on N recovery, or on final yields. N was mineralized from soil sources throughout the growth period, but mineralization was outweighed by ‘‘immobilization’’ of large amounts of N (around 30 kg/ha) in the soil, chiefly during May when crop growth was most rapid. This occurred on all sites with a crop, but not where the crop was absent. Measured losses of N2 and N2O were very small (<70 g/ha/dayN) on the site where they were measured, and no other evidence of loss or storage of N was found. The apparent recovery of fertilizer N at each site, was almost exactly explained by the amount immobilized during May. We conclude that the poor recovery of spring-applied N was due, not to losses as previously assumed but to temporary immobilization during the period of most active crop uptake. Immobilization was caused primarily by the presence of the crop.