EKSPLORASI BRYOPHYTA DI KAWASAN WISATA COBAN PELANGI, KECAMATAN PONCOKUSUMO-KABUPATEN MALANG

Bryophyta (lumut) adalah salah satu kelompok tumbuhan sederhana yang dapat ditemukan di area sedikit cahaya dan lembab. Mengingat pentingnya peran lumut bagi alam dan juga belum adanya data mengenai jenis-jenis lumut di kawasan wisata Coban Pelangi, maka penelitian ini dilakukan. Penelitian ini bertujuan untuk mengetahui jenis-jenis tumbuhan lumut yang tumbuh di kawasan wisata Coban Pelangi Poncokusumo, Kabupaten Malang-Jawa Timur. Penelitian ini merupakan penelitian kualitatif dengan metode eksploratif. Penelitian ini dilakukan di sepanjang jalur kawasan wisata setelah pintu masuk hingga sekitar air terjun. Hasil penelitian diperoleh 19 spesies lumut yang terdiri atas 1 spesies lumut tanduk, 12 spesies lumut daun dan 6 spesies lumut hati. Empat belas jenis lumut teridentifikasi hingga tingkat spesies dan 5 jenis teridentifikasi hingga tingkat genus. Sebanyak 6 spesies lumut ditemukan tumbuh pada substrat tanah, 6 spesies lumut yang tumbuh pada substrat pohon atau kayu, 6 spesies lumut yang tumbuh di substrat batu dan 1 spesies yang tumbuh pada substrat tanah dan batu.

IS THE FLOWER OF PULASAN (NEPHELIUM RAMBOUTAN-AKE) A PROTOGYNY OR PROTANDRY?

Pulasan is an androdioecious plant that has both male and hermaphrodite flowers on separate plants. The objectives of this study were to obtain data about the anatomical structure of pulasan flower and to investigate whether the pistil and the stamens of pulasan flower reach maturity at different times. The anatomical observation was done on compound flowers taken from three male trees and three hermaphrodite trees. Two compound flowers that still in buds, about to bloom and fully bloom were picked from each tree. Flowers were observed in a cross and longitudinal section. The results showed that the sepals of pulasan flower comprised of the uniseriate epidermis and multilayered polyhedral parenchymal cells. Stamen development started from the anther followed by the formation of the filament. The pollen of hermaphrodite pulasan flowers reached maturation earlier than the pistil. The pistil development started from the expansion of meristem cells in the center of the flower and ends with the warp of the stigma.

DIVERSITY OF HORNWORT IN MOUNT SLAMET (CENTRAL JAVA)

Recent more intensive explorations and collections of hornworts in Mt. Slamet indicated that six species can be recognized, namely Anthoceros punctatus, Folioceros fuciformis, Phaeoceros carolinianus, P. exiguus, Dendroceros difficilis, and Megaceros flagellaris. Since originally only one species D. difficilis was recorded from this area, this means that five species represent new records for the area, whereas P. exiguus is not only a new record for Java, but it is also a new record for Malesia.

GENETIC DIVERSITY OF LIMA BEAN (PHASEOLUS LUNATUS L.) FROM TIMOR ISLAND BASED ON MOLECULAR MARKER INTER-SIMPLE SEQUENCE REPEATS (ISSR)

Lima bean (Phaseolus lunatus L.) is one of the important legume vegetables in Indonesia. However, genetic information for these plants is still minimalized, especially on Timor Island. This study aims to analyze the genetic diversity of lima beans from Timor Island based on ISSR molecular markers. A total of 4 accessions of lima beans were analyzed using 3 ISSR primers to produce 15 polymorphic bands with an average of 68.18% polymorphism. The cluster analysis results use the Unweighted Pair Group Methods using Arithmetic averages (UPGMA) method to create a dendrogram that produces two main clusters. There were plain seed and pattern seed group with a similarity coefficient of 0.52. These results indicated that the genetic variation of the lima beans from Timor Island was high. Moreover, the result provides a sutable method for evaluating the genetic diversity of lima beans using the ISSR marker and important information of future lima bean breeding programs.

THE GENUS PYCNOPORUS IN INDONESIA

The genus Pycnoporus is a member of Phylum Basidiomycota. This fungus is one of polypore genera that is easily recognized from the orange color of the fruiting body and it likely has high morphological features. Currently, there are 207 number fungal specimens kept in Herbarium Bogoriense (BO) under the genus Pycnoporus, and they were identified as P. sa-nguineus (L.) Murrill, P. cinnabarinus (Jacq.) P. Karst., and Pycnoporus sp. To ensure that those species name of the Pycnoporus collections is correct, thus this study was carried out. Re-identification process on the fungal collections was done based on macro-micromorphological features of all Pycnoporus specimens. This study revealed that species Pycnoporus collections stored in BO represent 2 species: P.sanguineus (L.) Murrill and P. puniceus (Fr.) Ryvarden. In addition, some specimens under the name P. cinnabarinus should be validated as P. puniceus. This information will be very useful for fungal taxonomists, non-taxonomists, and people who work on Pycnoporus species from Indonesia.

ALSTONIA MACROPHYLLA (APOCYNACEAE): A NEW RECORD OF NATURALIZED SPECIES IN JAVA, INDONESIA

Surianto Effendi & Wendy A. Mustaqim. 2021. Alstonia macrophylla (Apocynaceae): A New Record of Naturalized Species in Java, Indonesia. Floribunda 6(6): 207–212. — Alstonia macrophylla (Apocynaceae), a Malesian widespread species tree, here for the first time is formally reported to be naturally found in Java. The report is based on plants growing in the Ciampea limestone hills, Ciampea sub-district, west of Bogor, West Java. Description, ecology, illustration and a brief discussion regarding the occurrence state of this species as naturalized species in Java are presented. A key to Alstonia species in Java is also given.

MITOTIC AND KARYOTYPE OF INDIGOFERA SUFFRUTICOSA MILL. IN CENTRAL JAVA

Wahyu Kusumawardani, Muzzazinah & Murni Ramli. 2021. Mitotic and Karyotype of Indigofera suffruticosa Mill. in Central Java. Floribunda 6(6): 213–219 . — Indigofera suffruticosa has been widely used by Indonesians, but its cytological information is still limited. This study was aimed to observe the mitotic phases and chromosome structures of I. suffruticosa. The analysis was performed on I. suffruticosa sampled from Magelang, Central Java. Mitotic phases and chromosomes’ structures were observed using root tips prepared with the squash method. The chromosome morphological structure was analyzed with karyotype maps using Image Raster, Corel Draw, and Microsoft Excel. The observation showed that I. suffruticosa were at various mitosis phases starting from prophase, pro-metaphase, metaphase, anaphase, and telophase. The chromosome in the pro-metaphase was analyzed for its karyotype morphology. The species has some diploid chromosomes (2n = 16). I. suffruticosa’s chromosomes have an average chromosome’ length of 2.08 µm with submetacentric and metacentric types. Data about the chromosome structure of I. suffruticosa from Indonesia could complement the novelty of the taxonomic classification information obtained by previous researchers.

CATATAN PADA RUMPUT KEBAR (OXALIDACEAE)

Yasper Michael Mambrasar, Taufik Mahendra, Megawati & Deby Arifiani. 2021. Catatan pada Rumput Kebar (Oxalidaceae). Floribunda 6(6): 220–224. — Rumput Kebar merupakan tumbuhan yang banyak dimanfaatkan sebagai obat dan penyubur kandungan. Sampai saat ini masih dijumpai penggunaan nama ilmiah untuk rumput kebar dengan menggunakan nama yang tidak valid. Di samping itu penggunaan nama lokal rumput Kebar juga mengarahkan pada dugaan bahwa jenis yang dimaksud masih berkerabat dengan rumput-rumputan (Poaceae). Hasil pengamatan spesimen herbarium dan studi literatur mendapatkan bahwa nama ilmiah rumput kebar adalah Biophytum umbraculum Welw dan B. petersianum Klotzsh merupakan sinonim. Daerah penyebaran rumput Kebar juga dibahas dalam artikel ini.

EPISTOLA BOTANICA: MARASMIELLUS SP. (BASIDIOMYCOTA, AGARICALES) FROM SIMEULEU ISLAND, SUMATRA, INDONESIA

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VARIASI CIRI MIKROMORFOLOGI BIJI BEGONIA (BEGONIACEAE) DI SUMATRA

Deden Girmansyah, Rugayah, Sulistijorini & Tatik Chikmawati. 2021. Variasi Ciri Mikromorfologi Biji Begonia (Begoniaceae) di Sumatra. Floribunda 6(6): 225–235. — Biji merupakan salah satu ciri penting dalam mempertelakan jenis Begonia. Karakter biji digunakan dalam deskripsi jenis Begonia adalah bentuk dan panjang biji, panjang collar cell (sel kerah), dan rasio collar cell dengan panjang biji. Untuk melengkapi karakter yang digunakan dalam deskripsi ini maka dilakukan pengamatan menggunakan SEM dengan alat table top SEM Hitachi TM3030. Hasil pengamatan dari 16 jenis dari empat seksi Begonia yang diamati menunjukan adanya perbedaan di antara empat seksi yang tumbuh di Sumatra. Perbedaan antara seksi terdapat pada bentuk antiklinal, area antar antiklinal dan pola kutikula. Ciri baru ini dapat digunakan sebagai data tambahan dalam melengkapi deskripsi Begonia.