scholarly journals MITOTIC AND KARYOTYPE OF INDIGOFERA SUFFRUTICOSA MILL. IN CENTRAL JAVA

Floribunda ◽  
2021 ◽  
Vol 6 (6) ◽  
Author(s):  
Wahyu  Kusumawardani ◽  
Muzzazinah Muzzazinah ◽  
Murni  Ramli
Keyword(s):  
Chromosome Structure ◽  
Morphological Structure ◽  
Chromosome Length ◽  
Taxonomic Classification ◽  
Root Tips ◽  
Microsoft Excel ◽  
Central Java ◽  
Classification Information ◽  
Karyotype Morphology

Wahyu Kusumawardani, Muzzazinah  & Murni Ramli. 2021. Mitotic and Karyotype of Indigofera suffruticosa Mill. in Central Java. Floribunda 6(6):  213–219 . — Indigofera suffruticosa has been widely used by Indonesians, but its cytological information is still limited. This study was aimed to observe the mitotic phases and chromosome structures of I. suffruticosa. The analysis was performed on I. suffruticosa sampled from Magelang, Central Java. Mitotic phases and chromosomes’ structures were observed using root tips prepared with the squash method. The chromosome morphological structure was analyzed with karyotype maps using Image Raster, Corel Draw, and Microsoft Excel. The observation showed that I. suffruticosa were at various mitosis phases starting from prophase, pro-metaphase, metaphase, anaphase, and telophase. The chromosome in the pro-metaphase was analyzed for its karyotype morphology. The species has some diploid chromosomes (2n = 16). I. suffruticosa’s chromosomes have an average chromosome’ length of 2.08 µm with submetacentric and metacentric types. Data about the chromosome structure of I. suffruticosa from Indonesia could complement the novelty of the taxonomic classification information obtained by previous researchers.  

scholarly journals Comparative cytogenetics in Cyclopogon (Orchidaceae)

Biologia ◽  
2013 ◽  
Vol 68 (1) ◽  
Author(s):  
Mauro Grabiele ◽  
Juan Cerutti ◽  
Diego Hojsgaard ◽  
Rubén Almada ◽  
Julio Daviña ◽  
...  
Keyword(s):  
Genome Size ◽  
Karyotype Analysis ◽  
Chromosome Length ◽  
The Novel ◽  
Comparative Cytogenetics ◽  
Molecular Approaches ◽  
Chromosome Fusions ◽  
Karyotype Morphology

AbstractA cytotaxonomical description of Cyclopogon (Spiranthinae, Orchidaceae) is carried out through a deep karyotype analysis of four species from NE Argentina. Distinctive karyotype parameters concerning the chromosomes number, morphology, size and symmetry and the genome size associate to each taxon. Cyclopogon calophyllus (2n = 2x =28; 18m + 10sm), C. congestus (2n = 2x = 32; 26m + 6sm), C. elatus (2n = 2x = 28; 18m + 10sm) and C. oliganthus (2n = 4x = 64; 40m + 24sm) possess symmetrical karyotypes (i-mean = 40.01–42.84; A 1 = 0.24–032; r>2 = 0.06–0.29) and excluding C. congestus (A 2 = 0.26; R = 2.62) unimodality is the rule (A 2 = 0.12–0.20; R = 1.73–1.92). Diploid taxa show a terminal macrosatellite in the m pair no. 2 (large arm) and share a comparable mean chromosome length (ca. 2.75 μm) and genome size (ca. 40 μm), superior to the tetraploid C. oliganthus (ca. 2 and 32 μm, respectively). The novel data added to preceding cytological, morphological and molecular approaches involving Cyclopogon and those related taxa of Spiranthinae largely based on x = 23 support the hypothesis that the unusual 2n and the karyotype morphology of Cyclopogon is an evolutionary advance within Spiranthinae with a basic reduction to x = 14 or 16 by chromosome fusions. A polyploid-dysploid series added to dibasic hybridization explain the extant 2n diversity though a paleopolyploid series on x = 7–8 is also possible.

Cytogenetic Diversity and Characterization of Vicia sativa Subspecies

2020 ◽  
Author(s):  
Berk Benlioglu
Keyword(s):  
Relative Length ◽  
Chromosome Length ◽  
Asymmetry Index ◽  
Close Relative ◽  
Vicia Sativa ◽  
Root Tips ◽  
Index Analysis ◽  
Wide Range ◽  
Karyotype Asymmetry ◽  
Intrachromosomal Asymmetry

Background: Vicia sativa L. is variable genus comprised of several subspecies. Close relative species and subspecies of the cultivated species are easily usable gene sources because they have gained resistance against a wide range of biotic and abiotic stresses. The main objectives of this study are to identify and describe the cytogenetical and karyological characteristics of subspecies in the Vicia sativa complex.Methods: The research material consisted of multiple entries collected from the five subspecies of nine taxa. All cytological observations made from root tips. Six chromosomal parameters (chromosome length, relative length, long arm length, short arm length, arm ratio and centromeric index) and five karyotype asymmetric parameters (difference in relative length, total form percentage, intrachromosomal asymmetry index, interchromosomal asymmetry index and mean centromeric asymmetry) were determined.Result: It was determined that the chromosome number of subspecies were 2n =10-12. The haploid chromosome lengths of subspecies were 15.86-33.88 µm and the average chromosome lengths varied between 2.64-5.65 µm. According to the intrachromosomal karyotype asymmetry index analysis, subsp. segetalis was the most asymmetric karyotype and subsp. sativa “Antalya” was the most symmetric karyotype. According to the interchromosomal karyotype asymmetry index analysis, subsp. angustifolia was the most asymmetric karyotype and subsp. nigra was the most symmetric karyotype.

A NEW SPECIES OF CENTAUREA (ASTERACEAE) FROM TURKEY

Phytotaxa ◽  
2016 ◽  
Vol 275 (2) ◽  
pp. 149 ◽  
Author(s):  
TUNA UYSAL ◽  
ERGIN HAMZAOĞLU ◽  
KUDDISI ERTUĞRUL ◽  
MERYEM BOZKURT
Keyword(s):  
New Species ◽  
Chromosome Length ◽  
Central Anatolia ◽  
Root Tips ◽  
Its Gene ◽  
Karyological Data ◽  
Karyotype Formula ◽  
Taxonomical Position ◽  
A New Species ◽  
Comprehensive Discussion

A new species of Centaurea (Asteraceae) from Turkey is described and illustrated. Centaurea nallihanense Uysal & Hamzaoğlu occurs on stony slopes of corrupted oak openings in the Province of Ankara in Central Anatolia. It seems to be similar to C. sect. Ptosimopappus but related to the Cheirolepis-Pseudoseridia complex. Diagnostic characters of similar taxa are provided and a comprehensive discussion is held on the basis of molecular and karyological data. The geographical distribution of the new species is mapped. The chromosome number of C. nallihanense, 2n = 2x = 18, counted in root tips, is also reported and illustrated. Its karyotype formula is 6m+3sm. The karyotype of C. nallihanense consists of mainly metacentric chromosomes with the length ranging from 1.113 to 1.771 µm, and the total haploid chromosome length of 12.909 µm. Also, the ITS gene region of the new species is amplified and sequenced to compare with the relatives and to identify the taxonomical position of the new species within Centaurea.

Chromosome Structure as Observed in Root Tips

Nature ◽  
1939 ◽  
Vol 144 (3645) ◽  
pp. 481-482 ◽  
Author(s):  
FLÃVIO RESENDE
Keyword(s):  
Chromosome Structure ◽  
Root Tips

scholarly journals Karyotype analysis of three species of Allium (Amaryllidaceae) from Thailand

2021 ◽  
Vol 22 (8) ◽  
Author(s):  
Surapon Saensouk ◽  
Piyaporn Saensouk
Keyword(s):  
Chromosome Numbers ◽  
Chromosome Structure ◽  
Karyotype Analysis ◽  
Root Tips ◽  
The Common

Abstract. Saensouk S, Saensouk P. 2021. Karyotype analysis of three species of Allium (Amaryllidaceae) from Thailand. Biodiversitas 22: 3458-3466. Comprehensive karyotype analysis of three species of Allium from Thailand has not been reported. This work aims to study karyotype analysis of three species of Allium from Thailand. It is confusing due to the use of the common name or local name or local Thai name and morphologies. The karyotype analysis of three species with two variations in the genus Allium from Thailand were studied from root tips and observed under a microscope then karyotype was obtained from 10 metaphase plates. The chromosome numbers of them were found 2n = 16. The karyotype formulas of them were constructed as 14m + 2st (Allium ascalonicum L. or shallot), 12m + 2sm + 2st (A. cepa L. or onion), 10m + 6sm (1 sat) (A. cepa L. or red onion), 10m + 4sm + 2st (A. sativum L. or big garlic or Chinese garlic) and 8m + 6sm + 2st (A. sativum L. or small garlic or Thai garlic). Chromosome structure differences among the three species appeared in the number of m, sm and st chromosomes and the satellites at the end of the short arm of A. cepa L. (red onion). This is new data on chromosome structure of the genus Allium. Moreover, the karyotype formulas, chromosome structures and satellites of this study should be used for classification of Allium from Thailand.

Sistem Komputerisasi Pengolahan data Penjualan dan Stok Pada Distributor alat alat Listrik

2020 ◽  
Vol 13 (1) ◽  
pp. 63-74
Author(s):  
Budi Hartono ◽  
Iwan Koerniawan
Keyword(s):  
Visual Basic ◽  
Database System ◽  
Private Company ◽  
Microsoft Excel ◽  
The Road ◽  
Central Java ◽  
Computer Based ◽  
On The Road ◽  
A Company ◽  
Visual Basic 6.0

In line with the development of science and technology today, a job that is done requires fast-paced data and information. By getting data and information quickly, the company has the ability to develop faster. Inventory is a very important part for a company. Inventory of goods is one of the tasks of logistics management in a company, namely support in the procurement of goods for the company. In order for this support to be utilized, it is necessary to plan and carry out in an integrated manner, which is interrelated and supports between related elements. CV Sinar Karya Ungaran is a private company which is engaged in the distributor of Electric tools. CV Sinar Karya is located on the road Dabo II / D30 RT6 Nitibuana, Ungaran Regency Semarang Tel. (024) 3584074 - 3584075. This company has a wide enough working area covering Central Java and DIY. Because the working area is quite extensive and the number of processes in and out of goods, the company must be more careful in calculating inventory. In the process of recording and counting inventory at this time the company is still using Microsoft Excel so there are still many obstacles. In the process of recording and counting inventory at this time the company still uses Microsoft Excel. One of the difficulties experienced is the frequent occurrence of errors in making inventory reports. Besides making reports takes longer because there is no computerized database system. With the development of technology, especially in the field of computers, the authors try to create a computer-based inventory program using Visual Basic 6.0. This is expected to help companies in making goods inventory more quickly and accurately.

scholarly journals The Diversity of the Dragonfly of Orthetrum Genus in Protected Area of Mount Prau, Central Java Indonesia

2017 ◽  
Vol 3 (1) ◽  
pp. 228
Author(s):  
Siti Mukhlisoh Setyawati ◽  
Puwowidodo Puwowidodo ◽  
Muh. Miftahul Huda ◽  
Bita Afriyanti Dewi
Keyword(s):  
Protected Area ◽  
River Flow ◽  
Morphological Structure ◽  
Body Color ◽  
Sample Analysis ◽  
Color Distribution ◽  
Male And Female ◽  
Morphological Variations ◽  
Central Java

<p style="text-align: justify;">Dragonfly of the genus Orthetrum is a dragonfly of the Lebullidae family. This dragonfly has a variety of types of morphological structure, body color, distribution and habitat. Habitat dragonfly of the genus Orthetrum is quite extensive, especially around lowland to upland waters. Protected Forest Mount Prau Central Java, is a rainforest that has a water flow appropriate for life dragonflies. It allows the Dragonflies of all kinds to live and thrive in the environment, including Orthetrum. This research was conducted to find out the types of Orthetrum that can be found in Protected Forest Area of Mount Prau, Central Java. Determination of sampling is done along the river flow at 3 stations with total sub plot of sample as much as 12 point, starting from near settlement flow up to 2100 m toward waterfall source. The results of the sample analysis showed morphological variations in the structure of the thorax and abdomen as well as the color variations and patterns on the thorax, abdomen, and wings. Identification of the dragonfly obtained four types of dragonfly Orthetrum with variations on the sex, the male and female sabina Orthetrum, Orthetrum caffrum males. Orthetrum testaceum male, male and female pruinosum Orthetrum, and Orthetrum male and female glaucum. ©2017 JNSMR UIN Walisongo. All rights reserved.</p>

scholarly journals Stage-resolved Hi-C analyses reveal meiotic chromosome organizational features influencing homolog alignment

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Wu Zuo ◽  
Guangming Chen ◽  
Zhimei Gao ◽  
Shuai Li ◽  
Yanyan Chen ◽  
...  
Keyword(s):  
Meiotic Prophase ◽  
Chromosome Structure ◽  
Meiotic Chromosome ◽  
Chromosome Length ◽  
Linc Complex ◽  
3D Genome ◽  
Homolog Pairing ◽  
Genomic Regions ◽  
Organizational Features ◽  
Chromosome Axis

AbstractDuring meiosis, chromosomes exhibit dramatic changes in morphology and intranuclear positioning. How these changes influence homolog pairing, alignment, and recombination remain elusive. Using Hi-C, we systematically mapped 3D genome architecture throughout all meiotic prophase substages during mouse spermatogenesis. Our data uncover two major chromosome organizational features varying along the chromosome axis during early meiotic prophase, when homolog alignment occurs. First, transcriptionally active and inactive genomic regions form alternating domains consisting of shorter and longer chromatin loops, respectively. Second, the force-transmitting LINC complex promotes the alignment of ends of different chromosomes over a range of up to 20% of chromosome length. Both features correlate with the pattern of homolog interactions and the distribution of recombination events. Collectively, our data reveal the influences of transcription and force on meiotic chromosome structure and suggest chromosome organization may provide an infrastructure for the modulation of meiotic recombination in higher eukaryotes.

Effect of Niacin on Mitochondria of Allium Cepa Root Cells

1967 ◽  
Vol 25 ◽  
pp. 78-79
Author(s):  
M. Arif Hayat
Keyword(s):  
Nicotinamide Adenine Dinucleotide ◽  
Hydrogen Transfer ◽  
Nicotinamide Adenine Dinucleotide Phosphate ◽  
Nicotinamide Adenine ◽  
Root Tips ◽  
Root Cells ◽  
Transfer Processes ◽  
Standard Procedures ◽  
A Cell ◽  
Critical Interest

Although it is recognized that niacin (pyridine-3-carboxylic acid), incorporated as the amide in nicotinamide adenine dinucleotide (NAD) or in nicotinamide adenine dinucleotide phosphate (NADP), is a cofactor in hydrogen transfer in numerous enzyme reactions in all organisms studied, virtually no information is available on the effect of this vitamin on a cell at the submicroscopic level. Since mitochondria act as sites for many hydrogen transfer processes, the possible response of mitochondria to niacin treatment is, therefore, of critical interest.Onion bulbs were placed on vials filled with double distilled water in the dark at 25°C. After two days the bulbs and newly developed root system were transferred to vials containing 0.1% niacin. Root tips were collected at ¼, ½, 1, 2, 4, and 8 hr. intervals after treatment. The tissues were fixed in glutaraldehyde-OsO4 as well as in 2% KMnO4 according to standard procedures. In both cases, the tissues were dehydrated in an acetone series and embedded in Reynolds' lead citrate for 3-10 minutes.

Some Biological Applications of High Voltage Electron Microscopy

1974 ◽  
Vol 32 ◽  
pp. 298-299
Author(s):  
Hans Ris
Keyword(s):  
High Voltage ◽  
Chromosome Structure ◽  
Nerve Fibers ◽  
Good Resolution ◽  
High Voltage Electron Microscopy ◽  
Voltage Electron ◽  
University Of Wisconsin ◽  
High Voltage Electron ◽  
One Year ◽  
The University

The High Voltage Electron Microscope Laboratory at the University of Wisconsin has been in operation a little over one year. I would like to give a progress report about our experience with this new technique. The achievement of good resolution with thick specimens has been mainly exploited so far. A cold stage which will allow us to look at frozen specimens and a hydration stage are now being installed in our microscope. This will soon make it possible to study undehydrated specimens, a particularly exciting application of the high voltage microscope.Some of the problems studied at the Madison facility are: Structure of kinetoplast and flagella in trypanosomes (J. Paulin, U. of Georgia); growth cones of nerve fibers (R. Hannah, U. of Georgia Medical School); spiny dendrites in cerebellum of mouse (Scott and Guillery, Anatomy, U. of Wis.); spindle of baker's yeast (Joan Peterson, Madison) spindle of Haemanthus (A. Bajer, U. of Oregon, Eugene) chromosome structure (Hans Ris, U. of Wisconsin, Madison). Dr. Paulin and Dr. Hanna are reporting their work separately at this meeting and I shall therefore not discuss it here.

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