scholarly journals Scalp Biopsy Specimens: Transverse vs Vertical Sections

2007 ◽  
Vol 143 (2) ◽  
Author(s):  
Carlos Garcia ◽  
Eduardo Poletti
2018 ◽  
Vol 78 (6) ◽  
pp. 1178-1184 ◽  
Author(s):  
Julio Jasso-Olivares ◽  
José Manuel Diaz-Gonzalez ◽  
Mariya Miteva

2021 ◽  
pp. 1-7 ◽  
Author(s):  
Suthinee Rutnin ◽  
Kumutnart Chanprapaph ◽  
Kallapan Pakornphadungsit ◽  
Kanchana Leerunyakul ◽  
Yingluck Visessiri ◽  
...  

<b><i>Introduction:</i></b> Scalp biopsy is a standard method for the definitive diagnosis of alopecia. The hair count parameters of each scalp area remain unclear. This study aimed to determine hair count values at different scalp locations from histopathology and to establish reference values for each part of the scalp. <b><i>Methods:</i></b> We obtained biopsy specimens from the frontal, vertex, temporoparietal, and occipital areas of the scalps of normal deceased subjects. All specimens were evaluated for the number of follicular units, hair counts, hair types, and stages of the hair cycle. <b><i>Results:</i></b> In total, 240 specimens were collected from 60 cadavers. Across all scalp sites, the temporoparietal area showed the lowest mean hair count, number of follicular units, terminal and vellus hairs, and terminal-to-vellus hair ratio. The average anagen-to-telogen hair ratio was comparable across all scalp sites. This study did not observe a significant association of hair parameters with gender differences or increasing age in all scalp areas. <b><i>Conclusions:</i></b> The present study revealed the diversity of the hair index among different scalp areas and suggested that normal hair count values should be separately standardized on each scalp region. Our findings may provide useful reference values for the histopathological evaluation of hair disorders in Asians.


1966 ◽  
Vol 35 (4) ◽  
pp. 355-361 ◽  
Author(s):  
R. S. SNELL

SUMMARY The effect of hydrocortisone acetate and adrenalectomy on the melanocytes and melanin in the skin of the anterior abdominal wall has been studied in pure black and pure red male guinea-pigs. The hormone was injected subcutaneously in doses of 1·6, 5 and 12·5 mg./day for 1 month into intact guinea-pigs and in doses of 2·5 mg./day into adrenalectomized guinea-pigs. Biopsy specimens of skin were taken before and after the hormone treatment and were processed with the Dopa reagent; both skin sheets and vertical sections were examined. In the adrenalectomized animals a further specimen of skin was taken some time after the cessation of the hormone treatment. Hydrocortisone and adrenalectomy produced no effect on the activity of the melanocytes. A statistical analysis of the melanocyte counts showed no change.


2002 ◽  
Vol 46 (2) ◽  
pp. 218-221 ◽  
Author(s):  
Hyun-Jeong Lee ◽  
Seog-Jun Ha ◽  
Joo-Han Lee ◽  
Jin-Wou Kim ◽  
Hyung-Ok Kim ◽  
...  

Author(s):  
Odell T. Minick ◽  
Hidejiro Yokoo ◽  
Fawzia Batti

To learn more of the nature and origin of alcoholic hyalin (AH), 15 liver biopsy specimens from patients with alcoholic hepatitis were studied in detail.AH was found not only in hepatocytes but also in ductular cells (Figs. 1 and 2), although in the latter location only rarely. The bulk of AH consisted of a randomly oriented network of closely packed filaments measuring about 150 Å in width. Bundles of filaments smaller in diameter (40-90 Å) were observed along the periphery of the main mass (Fig. 1), often surrounding it in a rim-like fashion. Fine filaments were also found close to the nucleus in both hepatocytes and biliary epithelial cells, the latter even though characteristic AH was not present (Figs. 3 and 4). Dispersed among the larger filaments were glycogen, RNA particles and profiles of endoplasmic reticulum. Dilated cisternae of endoplasmic reticulum were often conspicuous around the periphery of the AH mass. A limiting membrane was not observed.


Author(s):  
John H. L. Watson ◽  
C. N. Sun

That the etiology of Whipple's disease could be bacterial was first suggested from electron micrographs in 1960. Evidence for binary fission of the bacteria, their phagocytosis by histiocytes in the lamina propria, their occurrence between and within the cells of the epithelium and on the brush border of the lumen were reported later. Scanning electron microscopy has been applied by us in an attempt to confirm the earlier observations by the new technique and to describe the bacterium further. Both transmission and scanning electron microscopy have been used concurrently to study the same biopsy specimens, and transmission observations have been used to confirm those made by scanning.The locations of the brush borders, the columnar epithelial cells, the basement membrane and the lamina propria beneath it were each easily identified by scanning electron microscopy. The lamina propria was completely filled with the wiener-shaped bacteria, Fig. 1.


Author(s):  
John M. Basgen ◽  
Eileen N. Ellis ◽  
S. Michael Mauer ◽  
Michael W. Steffes

To determine the efficiency of methods of quantitation of the volume density of components within kidney biopsies, techniques involving a semi-automatic digitizing tablet and stereological point counting were compared.Volume density (Vv) is a parameter reflecting the volume of a component to the volume that contains the component, e.g., the fraction of cell volume that is made up of mitochondrial volume. The units of Vv are μm3 /μm3.Kidney biopsies from 15 patients were used. Five were donor biopsies performed at the time of kidney transplantation (patients 1-5, TABLE 1) and were considered normal kidney tissue. The remaining biopsies were obtained from diabetic patients with a spectrum of diabetic kidney lesions. The biopsy specimens were fixed and embedded according to routine electron microscogy protocols. Three glomeruli from each patient were selected randomly for electron microscopy. An average of 12 unbiased and systematic micrographs were obtained from each glomerulus and printed at a final magnification of x18,000.


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