scholarly journals Trypan Blue Exclusion Test of Cell Viability

2015 ◽  
Vol 111 (1) ◽  
Author(s):  
Warren Strober
Keyword(s):  
Cell Viability ◽  
Trypan Blue ◽  
Trypan Blue Exclusion ◽  
Trypan Blue Exclusion Test

scholarly journals The effect of different concentrations of Dimethyl sulfoxide (DMSO) and glycerol as cryoprotectant in preserving Vero cells

2017 ◽  
Vol 33 (1) ◽  
pp. 1-7
Author(s):  
MSI Siddiqui ◽  
R Parvin ◽  
M Giasuddin ◽  
SMZH Chowdhury ◽  
MR Islam ◽  
...  
Keyword(s):  
Cell Viability ◽  
Dimethyl Sulfoxide ◽  
Trypan Blue ◽  
Vero Cells ◽  
Total Cell ◽  
Trypan Blue Exclusion ◽  
Viability Analysis ◽  
Trypan Blue Exclusion Test ◽  
Maximum Cell

Different concentrations of dimethyl sulfoxide (DMSO) and glycerol were used for cryopreservation of Vero cells. After total cell count Vero cells were preserved in liquid nitrogen. Two frozen stocks were made simultaneously from the cell suspensions of same concentrations using DMSO or glycerol at concentrations of 2.5%, 5%, 10% and 15%. After six months of cryopreservation both frozen stocks were used providing same nutrients and environment for the viability of the Vero cells. The cell viability analysis was performed immediately after thawing by Trypan Blue Exclusion Test. Both cryoprotectants showed a protective effect on Vero cells. When Glycerol was used, a maximum cell viability rate of 89.4% and a lowest cell viability rate of 63% were achieved at concentrations of 10% and 2.5%, respectively. On the other hand, DMSO at a concentration of 10% had the highest effect on cryoprotectivity and showed highest cell viability (75%), while at 15% concentration it showed the lowest cell viability (53%). It is suggested that DMSO and glycerol are appropriate protective materials for the cryopreservation of Vero cells. The solutions at concentration of 10% of DMSO and glycerol could be the best choice of cryoprotectant for long-term (6 months) preservation of Vero cells.Bangl. vet. 2016. Vol. 33, No. 1, 1-7

Polydopamine and dopamine interfere with tetrazolium-based cytotoxicity assays and produce exaggerated cytocompatibility inferences

2021 ◽  
Author(s):  
Anand Kumar Awasthi ◽  
Sakshi Gupta ◽  
Kavthekar Rupesh Namdev ◽  
Aditi Banerjee ◽  
Aasheesh Srivastava
Keyword(s):  
Cell Viability ◽  
Trypan Blue ◽  
Reliable Estimate ◽  
Trypan Blue Exclusion ◽  
Cytotoxicity Assays ◽  
Trypan Blue Exclusion Assay

Polydopamine (PDA) and dopamine (DA) can spontaneously reduce MTT reagent to formazan, resulting in incorrect cell-viability inferences. The non-redox Trypan Blue exclusion assay provides a more reliable estimate of cell viability with PDA and DA.

scholarly journals Suitability of a Progenitor Cell-Enriching Device for In Vitro Applications

Coatings ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 146
Author(s):  
Antonio Celentano ◽  
Tami Yap ◽  
Giuseppe Pantaleo ◽  
Rita Paolini ◽  
Michael McCullough ◽  
...  
Keyword(s):  
Cell Viability ◽  
Time Course ◽  
Ex Vivo ◽  
Trypan Blue Exclusion ◽  
Trypan Blue Exclusion Test ◽  
Initial Reduction ◽  
Metal Wear ◽  
Class 1 ◽  
Electron Energy Loss

Rigenera® is a novel class-1 medical device that produces micro-grafts enriched of progenitors cells without ex vivo manipulation of donor tissues. The manufacturer’s protocol has been supported for a wide variety of clinical uses in the field of regenerative medicine. This study aimed to evaluate its potential use for in vitro cell models. Human primary oral fibroblasts were cultured under standard conditions and processed through Rigenera® over a time course of up to 5 min. Cell viability was assessed using a Trypan Blue exclusion test. It is possible to process fibroblasts through Rigenera® although an initial reduction of cell viability was observed. Additionally, debris was evident in the cell suspension of the processed samples. Scanning electron microscopy (SEM) microanalysis of the debris and electron energy-loss spectroscopy confirmed the presence of metal wear possibly due to the processing conditions used in this study. Interestingly, pore sizes within Rigeneracons® grids were found to range between 250–400 μm. This is the first report assessing the suitability of Rigenera® and Rigeneracons® for in vitro applications. Whilst Rigenera® workflow was found to be amenable to laboratory uses, our results strongly suggest that further research and development is necessary to support the utilization of this technology for enrichment of micro-graft derived cells and cell sorting in vitro.

In vitro photoradiation therapy of the rat 9L gliosarcoma

1986 ◽  
Vol 64 (5) ◽  
pp. 775-779 ◽  
Author(s):  
Douglas Hamilton ◽  
John D. S. McKean ◽  
John Tulip ◽  
Donald Boisvert ◽  
Judy Cummins
Keyword(s):  
Trypan Blue ◽  
Glioma Cells ◽  
Laser Source ◽  
Trypan Blue Exclusion ◽  
Content Type ◽  
9L Gliosarcoma ◽  
Trypan Blue Exclusion Test ◽  
9L Glioma ◽  
Fine Print

✓ The authors have investigated various factors involved in the photoradiation treatment of 9L glioma cells. The cells were grown in tissue culture and exposed to light from a laser source that allowed accurate quantitation of the light energy. Cell death was determined following treatment using the trypan blue exclusion test. It was shown that the treatment is very wavelength-dependent following the absorption spectrum of hematoporphyrin derivative (HPD). The absorption peaks in the lower part of the spectrum are more efficient than those of higher wavelengths. Photoradiation therapy is more effective the higher the concentration of HPD. Intensity of light is a very important factor in calculating the total dose of light necessary for this treatment.

scholarly journals Effect of nitric oxide release from NOR-3 on urea synthesis, viability and oxygen consumption of rat hepatocyte cultures

2007 ◽  
pp. 427-432
Author(s):  
R Chimenti ◽  
G Martino ◽  
S Mazzulla ◽  
S Sesti
Keyword(s):  
Nitric Oxide ◽  
Oxygen Consumption ◽  
Cell Viability ◽  
Urea Synthesis ◽  
Rat Hepatocyte ◽  
Trypan Blue Exclusion ◽  
No Donor ◽  
Nitric Oxide Release ◽  
Hepatocyte Cultures ◽  
Trypan Blue Exclusion Test

As nitric oxide is considered a mediator of liver oxidative metabolism during sepsis, we studied the effects of exogenous nitric oxide, produced by NO-donor, (+/-)-(E)-4-ethyl-2-[(E)-hydroxyimino]-5-nitro-3-hexenamide (NOR-3), on cell viability, urea biosynthesis and oxygen consumption in rat hepatocyte cultures. Nitric oxide release from NOR-3 was studied using 4,5-diaminofluorescein diacetate. Urea levels were measured by the spectrophotometric method. Cell viability was determined by the MTT test and trypan blue exclusion test, whereas oxygen consumption was measured by a polarographic technique. After 2 h treatment, NOR-3 induced an increase in the levels of nitric oxide. After 2 h of treatment and 24 h after the end of the treatment with NOR-3, both cell viability and urea synthesis were significantly reduced in comparison to the controls for NOR-3 concentrations equal to or greater than 50 microM. A reduction in oxygen consumption was observed in hepatocytes after 40 min treatment with 100 microM NOR-3, even if the cell viability was unchanged. Reduction of oxygen consumption is an early indicator of the metabolic alterations in hepatocytes exposed to nitric oxide. These findings suggest that nitric oxide accumulation acts on hepatocyte cultures inducing cell death and reduction of urea synthesis after 2 hours.

Secretion of Erythropoietin from Microencapsulated Rat Kidney Cells: Preliminary Results

1993 ◽  
Vol 16 (7) ◽  
pp. 557-560 ◽  
Author(s):  
J. Koo ◽  
T.M.S. Chang
Keyword(s):  
Cell Culture ◽  
Trypan Blue ◽  
Culture Media ◽  
Rat Kidney ◽  
Free Cell ◽  
Kidney Cells ◽  
Trypan Blue Exclusion ◽  
Trypan Blue Exclusion Test ◽  
Free Cells ◽  
The Media

Rat kidney epithelial cells were microencapsulated within alginate-poly(L)lysinealginate membrane. The microencapsulated cells were incubated using a culture media containing cobalt and another without cobalt. The viability was measured by trypan blue exclusion test. Secretion of erythropoietin (EPO) was measured by radioimmunoassay (RIA). Viability of free cells was 53%. The viability of microencapsulated cells increased to 72% after 12 days of incubation and remained at this level. Samples of the culture media were collected every 2 days for RIA. Samples within the microcapsules were collected by breaking the microcapsules open. RIA of these samples showed the following for the media containing cobalt. Between day 16 and day 32 the concentrations of EPO were 5.3 mU/ml inside and 18.3 mU/ml outside the microcapsule. The medium from the same number of free cells contained 21.2 mU/ml of EPO. Culture media without cobalt collected during the same period contained 1.8 mU/ml inside and 9.9 mU/ml outside the microcapsules. The free cell culture with this media during the same period contained 8.3 mU/ml.

scholarly journals Inhibitory Effects of Indirubin Derivatives on the Growth of HL-60 Leukemia Cells

2010 ◽  
Vol 5 (1) ◽  
pp. 1934578X1000500
Author(s):  
Nguyen Manh Cuong ◽  
Bui Huu Tai ◽  
Dang Hoang Hoan ◽  
Tran Thu Huong ◽  
Young Ho Kim ◽  
...  
Keyword(s):  
Cell Viability ◽  
Cancer Cells ◽  
Trypan Blue ◽  
Promyelocytic Leukemia ◽  
Leukemia Cells ◽  
Cytotoxic Activities ◽  
Inhibitory Effects ◽  
Trypan Blue Exclusion ◽  
Trypan Blue Exclusion Method ◽  
Exclusion Method

Six indirubin derivatives have been synthesized and their inhibitory effects on the growth of HL-60 human promyelocytic leukemia cells investigated. Cell viability was determined using the trypan blue exclusion method. Indirubin-3′-oxime (I-1) inhibited the growth of HL-60 cells with a GI50 value of 36.6 μM, whereas I-0, I-2, I-3, I-4 and I-6 showed only weak cytotoxic activities against HL-60 cancer cells with GI50 values in the range of 97.3 to over 100 μM. These results indicate that indirubin derivatives might be useful candidate agents for exploring potential antileukemic drugs.

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