Protein Carbonyl Measurement by Enzyme Linked Immunosorbent Assay

2011 ◽  
pp. 432-439 ◽  
Author(s):  
Betul Catalgol ◽  
Stefanie Grimm ◽  
Tilman Grune
Keyword(s):  
Immunosorbent Assay ◽  
Protein Carbonyl ◽  
Enzyme Linked Immunosorbent Assay

scholarly journals The Effect of Resistance Training on Malondialdehyde and Protein Carbonyl Concentration in the Heart Tissue of Rats Exposed to Stanozolol

2021 ◽  
Author(s):  
Ayat Arjmand ◽  
Bahram Abedi ◽  
Seyed Ali Hosseini
Keyword(s):  
Experimental Study ◽  
Resistance Training ◽  
Immunosorbent Assay ◽  
Normal Saline ◽  
Anabolic Steroids ◽  
Heart Tissue ◽  
Protein Carbonyl ◽  
Enzyme Linked Immunosorbent Assay ◽  
Weight Range ◽  
The Mean

Background: Consumption of anabolic steroids causes damage to various tissues, including the heart.  Objectives: This study aimed to investigate the effect of Resistance Training (RT) on Malondialdehyde (MDA) and Protein Carbonyl (PC) in the heart tissue of rats exposed to stanozolol (S). Methods: In this experimental study, 18 rats with the mean age of 8 weeks and weight range of 150 to 200 g were selected and divided into three groups of 6 rats: 1. Sham (normal saline consumption) (Sh), 2. S, and 3. S+RT. For 8 weeks, the S and S+RT groups received 5 mg/kg/d S, and the S+RT group performed 5 RT sessions per week. Measurement of MDA and PC in the heart tissue was performed with the enzyme-linked immunosorbent assay. Results: Stanozolol had a significant effect on increasing MAD (P=0.001) and PC (P=0.03) in the heart tissue. However, RT led to a decrease in MDA and PC in the heart tissue of rats exposed to S (P=0.001). Conclusion: It appears that S consumption leads to an increase in MDA and PC levels in the heart tissue, while RT can improve the elevated levels of MDA and PC.

Plasminogen Interactions with Immobilized Fibrinogen

1989 ◽  
Vol 62 (04) ◽  
pp. 1078-1082 ◽  
Author(s):  
Burt Adelman ◽  
Patricia Ouynn
Keyword(s):  
Immunosorbent Assay ◽  
Aminocaproic Acid ◽  
Enzyme Linked Immunosorbent Assay ◽  
Binding Regions ◽  
Dose Dependent Fashion ◽  
Epsilon Aminocaproic Acid ◽  
Dose Dependent

SummaryThis report describes the binding of plasminogen to fibrinogen adsorbed onto polystyrene wells. Binding was determined by enzyme linked immunosorbent assay. Both glu- and lys-plasminogen bound to immobilized fibrinogen in a dose-dependent fashion. However, more lys- than glu-plasminogen bound when equal concentrations of either were added to immobilized fibrinogen. Plasminogen binding was inhibited by epsilon aminocaproic acid indicating that binding was mediated via lysine-binding regions of plasminogen. Soluble fibrinogen added in excess of immobilized fibrinogen did not compete for plasminogen binding but fibrinogen fragments produced by plasmin digestion of fibrinogen did. Treatment of immobilized fibrinogen with thrombin caused a small but significant (p <0.01) increase in plasminogen binding. These studies demonstrate that immobilized fibrinogen binds both glu- and lys-plasminogen and that binding is mediated via lysine-binding regions. These interactions may facilitate plasminogen binding to fibrinogen adsorbed on to surfaces and to cells such as platelets which bind fibrinogen.

Alterations in Vascular Endothelial Cell-related Plasma Proteins In Thalassaemic Patients and their Correlation with Clinical Symptoms

1995 ◽  
Vol 74 (04) ◽  
pp. 1045-1049 ◽  
Author(s):  
P Butthep ◽  
A Bunyaratvej ◽  
Y Funahara ◽  
H Kitaguchi ◽  
S Fucharoen ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Endothelial Cell ◽  
Immunosorbent Assay ◽  
Plasma Proteins ◽  
Clinical Symptoms ◽  
Vascular Endothelial Cells ◽  
Vascular Endothelial Cell ◽  
Enzyme Linked Immunosorbent Assay ◽  
Vascular Endothelial ◽  
Leg Ulcers

SummaryAn increased level of plasma thrombomodulin (TM) in α- and β- thalassaemia was demonstrated using an enzyme-linked immunosorbent assay (ELISA). Nonsplenectomized patients with β-thalassaemia/ haemoglobin E (BE) had higher levels of TM than splenectomized cases (BE-S). Patients with leg ulcers (BE-LU) were found to have the highest increase in TM level. Appearance of larger platelets in all types of thalassaemic blood was observed indicating an increase in the number of younger platelets. These data indicate that injury of vascular endothelial cells is present in thalassaemic patients.

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