Comparison of first-voided urine specimens with endocervical swab specimens for enzyme-linked immunosorbent assay detection of Chlamydia trachomatis in women

1994 ◽  
Vol 3 (8) ◽  
pp. 672-675 ◽  
Author(s):  
J. A. Kellogg
Keyword(s):  
Chlamydia Trachomatis ◽  
Immunosorbent Assay ◽  
Enzyme Linked Immunosorbent Assay ◽  
Urine Specimens ◽  
Assay Detection

Enzyme-linked immunosorbent assay detection and bioactivity of Cry1Ab protein fragments

2016 ◽  
Vol 35 (12) ◽  
pp. 3101-3112 ◽  
Author(s):  
Vurtice C. Albright ◽  
Richard L. Hellmich ◽  
Joel R. Coats
Keyword(s):  
Immunosorbent Assay ◽  
Enzyme Linked Immunosorbent Assay ◽  
Cry1ab Protein ◽  
Protein Fragments ◽  
Assay Detection

scholarly journals Levels of Airborne Soybean Allergen (Gly m 1) in a Brazilian Soybean Production City: A Pilot Study

2020 ◽  
Vol 17 (15) ◽  
pp. 5381
Author(s):  
Cinthya Covessi Thom de Souza ◽  
Nelson Augusto Rosário Filho ◽  
Juliana Francis de Camargo ◽  
Ricardo Henrique Moreton Godoi
Keyword(s):  
Pilot Study ◽  
Immunosorbent Assay ◽  
Detection Method ◽  
Enzyme Linked Immunosorbent Assay ◽  
Soybean Production ◽  
Soy Production ◽  
Assay Detection ◽  
Soybean Allergen

Asthma epidemics have been shown to be related to where soybeans are loaded and handled, but data are scarce in the literature. This pilot study evaluated the levels of Gly m 1 in dust samples collected in Maringá, Brazil, a city with high soy production and processing. A dust impactor was used to collect seven isolated samples during 2015 and 2016. Samples were analyzed by an ELISA (enzyme-linked immunosorbent assay) detection method. Gly m 1 was found in all samples, ranging from 0.82–24.38 ng/m3 (median 2.41), regardless of the month or year evaluated. The levels of Gly m 1 were considered low, but the concentrations required to cause sensitization and symptoms are uncertain.

RAPID DIAGNOSIS OF TYPHOID FEVER BY ENZYME-LINKED IMMUNOSORBENT ASSAY DETECTION OF SALMONELLA SEROTYPE TYPHI ANTIGENS IN URINE

2004 ◽  
Vol 70 (3) ◽  
pp. 323-328 ◽  
Author(s):  
MOUSTAFA ABDEL FADEEL ◽  
YEHIA SULTAN ◽  
DAWLAT EL MELEGI ◽  
WILLIAM F. BIBB ◽  
BAHEIA REYAD ◽  
...  
Keyword(s):  
Typhoid Fever ◽  
Immunosorbent Assay ◽  
Rapid Diagnosis ◽  
Enzyme Linked Immunosorbent Assay ◽  
Salmonella Serotype ◽  
Assay Detection

Enzyme-Linked Immunosorbent Assay Detection of Melamine in Infant Formula and Wheat Food Products

2010 ◽  
Vol 73 (4) ◽  
pp. 701-707 ◽  
Author(s):  
ERIC A. E. GARBER ◽  
VICKERY A. BREWER
Keyword(s):  
Sample Preparation ◽  
Infant Formula ◽  
Immunosorbent Assay ◽  
Cyanuric Acid ◽  
Food Products ◽  
Cross Reactivity ◽  
Enzyme Linked Immunosorbent Assay ◽  
Limits Of Detection ◽  
Wheat Products ◽  
Assay Detection

The adulteration of food products with melamine to inflate the nitrogen content necessitates the establishment of analytical methods that can distinguish between proteinaceous ingredients and such adulterants. The specificity and ability to detect melamine by two commercial enzyme-linked immunosorbent assay (ELISA) kits were evaluated along with three protocols for sample preparation. Both ELISAs displayed cross-reactivity with ammeline, but neither was able to detect ammelide or cyanuric acid, indicating either a requirement for the 4,6-diamino-1,3,5-triazine structure or inability to bind 1,3,5-triazine-4,6-diones. The limits of detection for melamine in powder infant formula ranged from 0.2 to 3 μg/g depending on the ELISA kit and the method used to prepare the sample. The limits of detection for melamine in liquid infant formula and wheat products were <1 μg/ml and <2.5 μg/g, respectively. The ELISA kits provide an effective alternative for the analysis of samples suspected of containing melamine without relying on extensive sample preparation or expensive instrumentation.

Enzyme-linked immunosorbent assay detection of prostate-specific antigen messenger ribonucleic acid in prostate cancer

Urology ◽  
1999 ◽  
Vol 53 (1) ◽  
pp. 228-235 ◽  
Author(s):  
Senji Hoshi ◽  
Satsuki Kobayashi ◽  
Toshiko Takahashi ◽  
Ken-Ichi Suzuki ◽  
Sadafumi Kawamura ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Prostate Specific Antigen ◽  
Immunosorbent Assay ◽  
Ribonucleic Acid ◽  
Specific Antigen ◽  
Enzyme Linked Immunosorbent Assay ◽  
Messenger Ribonucleic Acid ◽  
Assay Detection

scholarly journals Competitive Binding Inhibition Enzyme-Linked Immunosorbent Assay That Uses the Secreted Aspartyl Proteinase of Candida albicans as an Antigenic Marker for Diagnosis of Disseminated Candidiasis

2003 ◽  
Vol 10 (5) ◽  
pp. 835-848 ◽  
Author(s):  
Christine J. Morrison ◽  
Steven F. Hurst ◽  
Errol Reiss
Keyword(s):  
Candida Albicans ◽  
Positive Predictive Value ◽  
Disease Progression ◽  
Immunosorbent Assay ◽  
Predictive Value ◽  
Significant Inhibition ◽  
Enzyme Linked Immunosorbent Assay ◽  
Binding Inhibition ◽  
Elisa Inhibition ◽  
Urine Specimens

ABSTRACT The secreted aspartyl proteinases (Saps) of Candida albicans have been implicated as virulence factors associated with adherence and tissue invasion. The potential use of proteinases as markers of invasive candidiasis led us to develop a competitive binding inhibition enzyme-linked immunosorbent assay (ELISA) to detect Sap in clinical specimens. Daily serum and urine specimens were collected from rabbits that had been immunosuppressed with cyclophosphamide and cortisone acetate and infected intravenously with 107 C. albicans blastoconidia. Disseminated infection was confirmed by organ culture and histopathology. Although ELISA inhibition was observed when serum specimens from these rabbits were used, more significant inhibition, which correlated with disease progression, occurred when urine specimens were used. Urine collected as early as 1 day after infection resulted in significant ELISA inhibition (mean inhibition ± standard error [SE] compared with preinfection control urine, 15.7% ± 2.7% [P < 0.01]), and inhibition increased on days 2 through 5 (29.4% ± 4.8% to 44.5% ± 3.5% [P < 0.001]). Urine specimens from immunosuppressed rabbits infected intravenously with Candida tropicalis, Candida parapsilosis, Candida krusei, Cryptococcus neoformans, Aspergillus fumigatus, or Staphylococcus aureus were negative in the assay despite culture-proven dissemination. Nonimmunosuppressed rabbits receiving oral tetracycline and gentamicin treatment were given 2 × 108 C. albicans blastoconidia orally or intraurethrally to establish colonization of the gastrointestinal tract or bladder, respectively, without systemic dissemination; urine specimens from these rabbits also gave negative ELISA results. Dissemination to the kidney and spleen occurred in one rabbit challenged by intragastric inoculation, and urine from this rabbit demonstrated significant inhibition in the ELISA (mean inhibition ± SE by day 3 after infection, 32.9% ± 2.7% [P < 0.001]). The overall test sensitivity was 83%, the specificity was 92%, the positive predictive value was 84%, the negative predictive value was 91%, and the efficiency was 89% (166 urine samples from 33 rabbits tested). The specificity, positive predictive value, and efficiency could be increased to 97, 95, and 92%, respectively, if at least two positive test results were required for a true positive designation. The ELISA was sensitive and specific for the detection of Sap in urine specimens from rabbits with disseminated C. albicans infection, discriminated between colonization and invasive disease, reflected disease progression and severity, and has the potential to be a noninvasive means to diagnose disseminated candidiasis.

scholarly journals Immunology: Chlamydia trachomatis antibody titres by enzyme-linked immunosorbent assay are useful in predicting severity of adnexal adhesion

1996 ◽  
Vol 11 (11) ◽  
pp. 2418-2421 ◽  
Author(s):  
M. Tanikawa ◽  
T. Harada ◽  
C. Katagiri ◽  
Y. Onohara ◽  
S. Yoshida ◽  
...  
Keyword(s):  
Chlamydia Trachomatis ◽  
Immunosorbent Assay ◽  
Enzyme Linked Immunosorbent Assay ◽  
Antibody Titres
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