Oligomycin A Induces Apoptosis‐to‐Pyroptosis Switch against Melanoma with Sensitized Immunotherapy

1. The effects of dicyclohexylcarbodi-imide, oligomycin A and aurovertin on enzyme systems related to respiratory-chain phosphorylation were compared. Dicyclohexylcarbodi-imide and oligomycin A have very similar functional effects, giving 50% inhibition of ATP-utilizing and ATP-generating systems at concentrations below 0·8nmole/mg. of submitochondrial-particle protein. Aurovertin is a more potent inhibitor of ATP synthesis, giving 50% inhibition at 0·2nmole/mg. of protein. However, aurovertin is a less potent inhibitor of ATP-utilizing systems: the ATP-driven energy-linked nicotinamide nucleotide transhydrogenase is 50% inhibited at 3·0nmoles/mg. of protein and the ATP-driven reduction of NAD+ by succinate is 50% inhibited at 0·95nmole/mg. of protein. 2. With EDTA-particles (prepared by subjecting mitochondria to ultrasonic radiation at pH9 in the presence of 2mm-EDTA) the maximum stimulation of the ATP-driven partial reactions is effected by similar concentrations of oligomycin A and dicylcohexylcarbodi-imide, but the latter is less effective. The stimulatory effects of suboptimum concentrations of dicyclohexylcarbodi-imide and oligomycin A are additive. Aurovertin does not stimulate these reactions or interfere with the stimulation by the other inhibitors. 3. Dicyclohexylcarbodi-imide and oligomycin A stimulate the aerobic energy-linked nicotinamide nucleotide transhydrogenase of EDTA-particles, but the optimum concentration is higher than that required for the ATP-driven partial reactions. Aurovertin has no effect on this reaction. 4. The site of action of dicyclohexylcarbodi-imide is in CF0, the mitochondrial fraction that confers oligomycin sensitivity on F1 mitochondrial adenosine triphosphatase.

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Red LED Light Acts on the Mitochondrial Electron Chain of Donkey Sperm and Its Effects Depend on the Time of Exposure to Light

Frontiers in Cell and Developmental Biology ◽

10.3389/fcell.2020.588621 ◽

2020 ◽

Vol 8 ◽

Cited By ~ 1

Author(s):

Jaime Catalán ◽

Marion Papas ◽

Lina Trujillo-Rojas ◽

Olga Blanco-Prieto ◽

Sebastián Bonilla-Correal ◽

...

Keyword(s):

Red Light ◽

Specific Inhibitor ◽

Motile Sperm ◽

Light Stimulation ◽

Led Light ◽

Mitochondrial Atp Synthase ◽

Red Led ◽

Oligomycin A ◽

Rate Of Oxygen Consumption ◽

Stimulation Of

This work aimed to investigate how stimulation of donkey sperm with red LED light affects mitochondrial function. For this purpose, freshly diluted donkey semen was stimulated with red light for 1, 5, and 10 min, in the presence or absence of oligomycin A (Omy A), a specific inhibitor of mitochondrial ATP synthase, or FCCP, a specific disruptor of mitochondrial electron chain. The results obtained in the present study indicated that the effects of red LED light on fresh donkey sperm function are related to changes in mitochondria function. In effect, irradiation of donkey sperm resulted in an increase in mitochondrial membrane potential (MMP), the activity of cytochrome C oxidase and the rate of oxygen consumption. In addition, in the absence of oligomycin A and FCCP, light-stimulation augmented the average path velocity (VAP) and modified the structure of motile sperm subpopulations, increasing the fastest and most linear subpopulation. In contrast, the presence of either Omy A or FCCP abolished the aforementioned effects. Interestingly, our results also showed that the effects of red light depend on the exposure time applied, as indicated by the observed differences between irradiation protocols. In conclusion, our results suggest that exposing fresh donkey sperm to red light modulates the function of their mitochondria through affecting the activity of the electron chain. However, the extent of this effect depends on the irradiation pattern and does not exclude the existence of other mechanisms, such as those related to thermotaxis.

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Isolation, identification, and antifungal activity of a macrolide antibiotic, oligomycin A, produced by Streptomyces libani

Canadian Journal of Botany ◽

10.1139/cjb-77-6-850 ◽

1999 ◽

Vol 77 (6) ◽

pp. 850-858 ◽

Cited By ~ 2

Author(s):

Beom Seok Kim ◽

Surk Sik Moon ◽

Byung Kook Hwang

Keyword(s):

Antifungal Activity ◽

Macrolide Antibiotic ◽

Oligomycin A

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Concerted use of two-dimensional NMR techniques in theab initio assignment of complex spectra: Complete proton and carbon-13 assignment of oligomycin A

Magnetic Resonance in Chemistry ◽

10.1002/mrc.1260230815 ◽

1985 ◽

Vol 23 (8) ◽

pp. 676-683 ◽

Cited By ~ 18

Author(s):

Gareth A. Morris ◽

Michelle S. Richards

Keyword(s):

Two Dimensional ◽

Oligomycin A ◽

Nmr Techniques

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Functional characterization of CYP107W1 from Streptomyces avermitilis and biosynthesis of macrolide oligomycin A

Archives of Biochemistry and Biophysics ◽

10.1016/j.abb.2015.03.025 ◽

2015 ◽

Vol 575 ◽

pp. 1-7 ◽

Cited By ~ 11

Author(s):

Songhee Han ◽

Tan-Viet Pham ◽

Joo-Hwan Kim ◽

Young-Ran Lim ◽

Hyoung-Goo Park ◽

...

Keyword(s):

Functional Characterization ◽

Streptomyces Avermitilis ◽

Oligomycin A

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The role of mitochondria linked fatty-acid uptake-driven adipogenesis in Graves’ Orbitopathy

Endocrinology ◽

10.1210/endocr/bqab188 ◽

2021 ◽

Author(s):

Lei Zhang ◽

Pavandeep Rai ◽

Satomi Miwa ◽

Mohd Shazli Draman ◽

D Aled Rees ◽

...

Keyword(s):

Adipose Tissue ◽

Fatty Acid ◽

Culture Medium ◽

Fatty Acid Uptake ◽

Therapeutic Interventions ◽

Acid Uptake ◽

Atp Production ◽

Oligomycin A ◽

Graves Orbitopathy

Abstract Context Depot-specific expansion of orbital-adipose-tissue (OAT) in Graves’ Orbitopathy (GO, an autoimmune condition producing proptosis, visual impairment and reduced quality of life) is associated with fatty-acid (FA) uptake-driven adipogenesis in preadipocytes/fibroblasts (PFs). Objective A role for mitochondria in OAT-adipogenesis in GO. Design, Setting, Participants Confluent PFs from healthy OAT (OAT-H), OAT from GO (OAT-GO) and white-adipose-tissue in culture-medium compared with culture-medium containing a mixed hormonal-cocktail as adipogenic-medium (ADM); or culture-medium containing FA-supplementation, oleate:palmitate:linoleate (45:30:25%) with/without different concentration of mitochondrial bio-substrate ADP/GDP, AICAR (adenosine-analog) or inhibitor oligomycin-A for 17 days. Main outcome measures Oil-Red-O staining and foci-count of differentiated adipocytes for in-vitro adipogenesis; flow-cytometry, relative-QPCR, MTS-assay/10 6 cells, total cellular-ATP detection kit and Seahorse-XFe96-Analyzer for mitochondria and OXPHOS/Glycolysis-ATP production analysis. Results During early adipogenesis before adipocyte formation (day-0,4&7), we observed OAT-specific cellular ATP-production via mitochondrial-OXPHOS in PFs from both OAT-H/OAT-GO, and substantially disrupted OXPHOS-ATP/Glycolysis-ATP production in PFs from OAT-GO, e.g. 40% reduction in OXPHOS-ATP and trend-increased Glycolysis-ATP production on day-4&7 compared with day-0, which contrasted with the stable levels in OAT-H.FA-supplementation in culture-medium triggered adipogenesis in PFs from both OAT-H/OAT-GO, which was substantially enhanced by 1mM GDP reaching 7-18% of ADM-adipogenesis. The FA-uptake-driven adipogenesis was diminished by oligomycin-A but unaffected by treatment with ADP or AICAR. Furthermore, we observed significant positive correlation between FA-uptake-driven adipogenesis by GDP and the ratios of OXPHOS-ATP/Glycolysis-ATP through adipogenesis of PFs from OAT-GO. Conclusions Our study confirmed that FA-uptake can drive OAT-adipogenesis and revealed a fundamental role for mitochondria-OXPHOS in GO development, which provides potential for therapeutic interventions.

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Synthesis and Anti-Actinomycotic Activity of the Oligomycin A Thiocyanato Derivative Modified at 2-Oxypropyl Side Chain

Macroheterocycles ◽

10.6060/mhc151084s ◽

2015 ◽

Vol 8 (4) ◽

pp. 424-428 ◽

Cited By ~ 6

Author(s):

Lyudmila N. Lysenkova ◽

Ivan A. Godovikov ◽

Alexander M. Korolev ◽

Valery N. Danilenko ◽

Olga B. Bekker ◽

...

Keyword(s):

Side Chain ◽

Oligomycin A

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MITOCHONDRIAL GENETIC ANALYSIS BY ZYGOTE CELL LINEAGES IN SACCHAROMYCES CEREVISIAE

Genetics ◽

10.1093/genetics/73.3.367 ◽

1973 ◽

Vol 73 (3) ◽

pp. 367-377

Author(s):

D Wilkie ◽

D Y Thomas

Keyword(s):

Saccharomyces Cerevisiae ◽

Genetic Analysis ◽

Pedigree Analysis ◽

Cell Lineages ◽

Mitochondrial Markers ◽

Yeast Strains ◽

Oligomycin A ◽

Multiple Recombination

ABSTRACT Yeast strains were constructed carrying multiple mitochondrial markers conferring resistance to the inhibitors erythromycin, chloramphenicol, paromomycin and oligomycin. A pedigree analysis of two crosses was made by micromanipulating buds from zygotes. The first few daughter buds isolated from the zygotes sometimes gave rise to diploid clones which had a mixture of mitochondrial types. All possible classes of mitochondrial parental and recombinant types were found although they never appeared all together as the progeny from a single zygote. It was inferred that multiple recombination events took place in zygotes and in some of the buds derived from them. After removal of the first four or so daughter buds, subsequent buds from the zygote carried one mitochondrial type only. In cross I in which three markers were analyzed this was most frequently one of the parental types. In cross II (involving four mitochondrial markers) the later buds from the zygotes were frequently of recombinant mitochondrial type.

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