Stem Cell Substrates: Pulsed-Electromagnetic-Field-Assisted Reduced Graphene Oxide Substrates for Multidifferentiation of Human Mesenchymal Stem Cells (Adv. Healthcare Mater. 16/2016)

2016 ◽  
Vol 5 (16) ◽  
pp. 2144-2144
Author(s):  
Ki-Taek Lim ◽  
Hoon Seonwoo ◽  
Kyung Soon Choi ◽  
Hexiu Jin ◽  
Kyung-Je Jang ◽  
...  
2016 ◽  
Vol 33 (1) ◽  
pp. 201-211 ◽  
Author(s):  
Yun-Kyong Choi ◽  
Enerelt Urnukhsaikhan ◽  
Hee-Hoon Yoon ◽  
Young-Kwon Seo ◽  
Hyunjin Cho ◽  
...  

PLoS ONE ◽  
2018 ◽  
Vol 13 (6) ◽  
pp. e0199046 ◽  
Author(s):  
Nora Bloise ◽  
Loredana Petecchia ◽  
Gabriele Ceccarelli ◽  
Lorenzo Fassina ◽  
Cesare Usai ◽  
...  

Nanoscale ◽  
2015 ◽  
Vol 7 (27) ◽  
pp. 11642-11651 ◽  
Author(s):  
Jong Ho Lee ◽  
Yong Cheol Shin ◽  
Oh Seong Jin ◽  
Seok Hee Kang ◽  
Yu-Shik Hwang ◽  
...  

Reduced graphene oxide-coated hydroxyapatite (rGO-coated HAp) composites stimulated the spontaneous osteogenesis in human mesenchymal stem cells in the absence of osteoinductive agents.


2015 ◽  
Vol 15 (10) ◽  
pp. 7966-7970 ◽  
Author(s):  
Linhua Jin ◽  
Jong Ho Lee ◽  
Oh Seong Jin ◽  
Yong Cheol Shin ◽  
Min Jeong Kim ◽  
...  

Osteoprogenitor cells play a significant role in the growth or repair of bones, and have great potential as cell sources for regenerative medicine and bone tissue engineering, but control of their specific differentiation into bone cells remains a challenge. Graphene-based nanomaterials are attractive candidates for biomedical applications as substrates for stem cell (SC) differentiation, scaffolds in tissue engineering, and components of implant devices owing to their biocompatible, transferable and implantable properties. This study examined the enhanced osteogenic differentiation of human mesenchymal stem cells (hMSCs) by reduced graphene oxide (rGO) nanoparticles (NPs), and rGO NPs was prepared by reducing graphene oxide (GO) with a hydrazine treatment followed by annealing in argon and hydrogen. The cytotoxicity profile of each particle was examined using a water-soluble tetrazolium-8 (WST-8) assay. At different time-points, a WST-8 assay, alkaline phosphatase (ALP) activity assay and alizarin red S (ARS) staining were used to determine the effects of rGO NPs on proliferation, differentiation and mineralization, respectively. The results suggest that graphene-based materials have potential as a platform for stem cells culture and biomedicalapplications.


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