Dual Responsive Surfaces Based on Host-Guest Interaction for Dynamic Mediation of Cell-Substrate Interaction and Cell Migration

2016 ◽  
Vol 4 (1) ◽  
pp. 1500865 ◽  
Author(s):  
Tanchen Ren ◽  
Yelin Ni ◽  
Wang Du ◽  
Shan Yu ◽  
Zhengwei Mao ◽  
...  
Keyword(s):  
Cell Migration ◽  
Substrate Interaction ◽  
Dual Responsive ◽  
Cell Substrate ◽  
Responsive Surfaces

8C24 Non-invasive control of cell migration based on cell-substrate interaction

2012 ◽  
Vol 2012.24 (0) ◽  
pp. _8C24-1_-_8C24-2_
Author(s):  
Hiromi MIYOSHI ◽  
Jungmyoung JU ◽  
Sang Min LEE ◽  
Dong Jin CHO ◽  
Jong Soo KO ◽  
...  
Keyword(s):  
Cell Migration ◽  
Non Invasive ◽  
Substrate Interaction ◽  
Cell Substrate ◽  
Invasive Control

scholarly journals KIAA0319 influences cilia length, cell migration and mechanical cell-substrate interaction

2019 ◽  
Author(s):  
Rebeca Diaz ◽  
Nils Michael Kronenberg ◽  
Angela Martinelli ◽  
Philipp Liehm ◽  
Andrew Clive Riches ◽  
...  
Keyword(s):  
Cell Migration ◽  
Animal Models ◽  
Epithelial Cells ◽  
Genetic Studies ◽  
Substrate Interaction ◽  
Cell Substrate ◽  
Rescue Experiment ◽  
Cilia Formation ◽  
First Time ◽  
Knockout Model

Following its association with dyslexia in multiple genetic studies, the KIAA0319 gene has been extensively investigated in different animal models but its function in neurodevelopment remains poorly understood. We developed the first cellular knockout model for KIAA0319 via CRISPR-Cas9n to investigate its role in processes suggested but not confirmed in previous studies, including cilia formation and cell migration. We found that KIAA0319 knockout increased cilia length and accelerated cell migration. Using Elastic Resonator Interference Stress Microscopy (ERISM), we detected an increase in cellular force for the knockout cells that was restored by a rescue experiment. Combining ERISM and immunostaining we show that KIAA0319 depletion reduces the number of podosomes formed by the cells. Our results suggest an involvement of KIAA0319 in cilia biology and force regulation and show for the first time that podosomes exert highly dynamic, piconewton vertical forces in epithelial cells.

scholarly journals KIAA0319 influences cilia length, cell migration and mechanical cell–substrate interaction

2022 ◽  
Vol 12 (1) ◽  
Author(s):  
Rebeca Diaz ◽  
Nils M. Kronenberg ◽  
Angela Martinelli ◽  
Philipp Liehm ◽  
Andrew C. Riches ◽  
...  
Keyword(s):  
Cell Migration ◽  
Animal Models ◽  
Retinal Pigment ◽  
Genetic Studies ◽  
Substrate Interaction ◽  
Cell Substrate ◽  
Rescue Experiment ◽  
Cilia Formation ◽  
Knockout Model

AbstractFollowing its association with dyslexia in multiple genetic studies, the KIAA0319 gene has been extensively investigated in different animal models but its function in neurodevelopment remains poorly understood. We developed the first human cellular knockout model for KIAA0319 in RPE1 retinal pigment epithelia cells via CRISPR-Cas9n to investigate its role in processes suggested but not confirmed in previous studies, including cilia formation and cell migration. We observed in the KIAA0319 knockout increased cilia length and accelerated cell migration. Using Elastic Resonator Interference Stress Microscopy (ERISM), we detected an increase in cellular force for the knockout cells that was restored by a rescue experiment. Combining ERISM and immunostaining we show that RPE1 cells exert highly dynamic, piconewton vertical pushing forces through actin-rich protrusions that are surrounded by vinculin-rich pulling sites. This protein arrangement and force pattern has previously been associated to podosomes in other cells. KIAA0319 depletion reduces the fraction of cells forming these actin-rich protrusions. Our results suggest an involvement of KIAA0319 in cilia biology and cell–substrate force regulation.

scholarly journals Hyaluronan–CD44 interaction hampers migration of osteoclast-like cells by down-regulating MMP-9

2002 ◽  
Vol 158 (6) ◽  
pp. 1133-1144 ◽  
Author(s):  
Paola Spessotto ◽  
Francesca Maria Rossi ◽  
Massimo Degan ◽  
Raffaele Di Francia ◽  
Roberto Perris ◽  
...  
Keyword(s):  
Cell Migration ◽  
Bone Resorption ◽  
Cell Motility ◽  
Binding Affinity ◽  
Antisense Oligonucleotides ◽  
Substrate Binding ◽  
Down Regulation ◽  
Multinucleated Cells ◽  
Cell Substrate

Osteoclast (OC) precursors migrate to putative sites of bone resorption to form functionally active, multinucleated cells. The preOC FLG 29.1 cells, known to be capable of irreversibly differentiating into multinucleated OC-like cells, displayed several features of primary OCs, including expression of specific integrins and the hyaluronan (HA) receptor CD44. OC-like FLG 29.1 cells adhered to and extensively migrated through membranes coated with fibronectin, vitronectin, and laminins, but, although strongly binding to HA, totally failed to move on this substrate. Moreover, soluble HA strongly inhibited OC-like FLG 29.1 cell migration on the permissive matrix substrates, and this behavior was dependent on its engagement with CD44, as it was fully restored by function-blocking anti-CD44 antibodies. HA did not modulate the cell–substrate binding affinity/avidity nor the expression levels of the corresponding integrins. MMP-9 was the major secreted metalloproteinase used by OC-like FLG 29.1 cells for migration, because this process was strongly inhibited by both TIMP-1 and GM6001, as well as by MMP-9–specific antisense oligonucleotides. After HA binding to CD44, a strong down-regulation of MMP-9 mRNA and protein was detected. These findings highlight a novel role of the HA–CD44 interaction in the context of OC-like cell motility, suggesting that it may act as a stop signal for bone-resorbing cells.

scholarly journals Assessing Cell-Substrate Interaction with Dissipation Monitoring Function of the QCM-D

2017 ◽  
Vol 112 (3) ◽  
pp. 434a-435a ◽  
Author(s):  
Jennifer Chen ◽  
Lynn Penn ◽  
Ning Xi ◽  
Jun Xi
Keyword(s):  
Substrate Interaction ◽  
Cell Substrate

Effects of Friction Coefficient and Receptor Number on Cell-Substrate Interactions During Migration

2010 ◽  
Author(s):  
Henry C. Wong ◽  
William C. Tang
Keyword(s):  
Cell Migration ◽  
Cancer Metastasis ◽  
Element Model ◽  
Biological Tissues ◽  
Substrate Interactions ◽  
Strain Behavior ◽  
Structural Support ◽  
Research Areas ◽  
Cell Substrate ◽  
A Cell

Biological tissues are composed of cells that adhere to the extracellular matrix (ECM) via cell-surface integrin receptors that bind to specific proteins, such as fibronectin, embedded in the matrix. In this manner, the ECM functions as a structural support for the attached cells, and mechanical forces are able to be transmitted from the cell to the ECM and vice versa [1]. Cell migration, a process that is highly dependent on these mechanical interactions, is important for many normal biological processes and diseases that occur in the human body, which include embryonic development, immune response, would healing, and cancer invasion [2]. Though many continuum models of cell migration have been proposed, there is still a need for a model that can be used to quantitatively understand the mechanical factors that can influence the movement of a cell on a substrate. This would be invaluable to the research areas of tissue engineering as well as cancer metastasis. We utilized a finite element model to elucidate the mechanism of cell-substrate interactions for a cell that consistently migrates in a single direction. Our model follows the approach taken by Gracheva and Othmer [2], but we extended their model to describe two-dimensional plane strain behavior.

Minimization of cell-substrate interaction using suspended microstructured meshes initiates cell sheet formation by self-assembly organization

2016 ◽  
Vol 2 (6) ◽  
pp. 065019 ◽  
Author(s):  
Kennedy Omondi Okeyo ◽  
Osamu Kurosawa ◽  
Hidehiro Oana ◽  
Hidetoshi Kotera ◽  
Masao Washizu
Keyword(s):  
Self Assembly ◽  
Cell Sheet ◽  
Substrate Interaction ◽  
Cell Substrate

scholarly journals The interplay of cell–cell and cell–substrate adhesion in collective cell migration

2014 ◽  
Vol 11 (100) ◽  
pp. 20140684 ◽  
Author(s):  
Chenlu Wang ◽  
Sagar Chowdhury ◽  
Meghan Driscoll ◽  
Carole A. Parent ◽  
S. K. Gupta ◽  
...  
Keyword(s):  
Cell Migration ◽  
Cell Surface ◽  
Actin Polymerization ◽  
Collective Cell Migration ◽  
Cell Contacts ◽  
Substrate Adhesion ◽  
Cell Substrate ◽  
Cell Shapes ◽  
Cell Substrate Adhesion ◽  
Cell Cell

Collective cell migration often involves notable cell–cell and cell–substrate adhesions and highly coordinated motion of touching cells. We focus on the interplay between cell–substrate adhesion and cell–cell adhesion. We show that the loss of cell-surface contact does not significantly alter the dynamic pattern of protrusions and retractions of fast migrating amoeboid cells ( Dictyostelium discoideum ), but significantly changes their ability to adhere to other cells. Analysis of the dynamics of cell shapes reveals that cells that are adherent to a surface may coordinate their motion with neighbouring cells through protrusion waves that travel across cell–cell contacts. However, while shape waves exist if cells are detached from surfaces, they do not couple cell to cell. In addition, our investigation of actin polymerization indicates that loss of cell-surface adhesion changes actin polymerization at cell–cell contacts. To further investigate cell–cell/cell–substrate interactions, we used optical micromanipulation to form cell–substrate contact at controlled locations. We find that both cell-shape dynamics and cytoskeletal activity respond rapidly to the formation of cell–substrate contact.

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