scholarly journals Inhibition of Collagenase Q1 of Bacillus cereus as a Novel Antivirulence Strategy for the Treatment of Skin‐Wound Infections

2022 ◽  
pp. 2100222
Author(s):  
Alaa Alhayek ◽  
Essak S. Khan ◽  
Esther Schönauer ◽  
Tobias Däinghaus ◽  
Roya Shafiei ◽  
...  
Keyword(s):  
Bacillus Cereus ◽  
Skin Wound ◽  
Wound Infections

scholarly journals Pathogenic Role of Bacillus Cereus in Wound Infections in the Tropics

1987 ◽  
Vol 80 (8) ◽  
pp. 480-481 ◽  
Author(s):  
M S Dryden
Keyword(s):  
Bacillus Cereus ◽  
Clinical Findings ◽  
Costa Rican ◽  
High Rate ◽  
Wound Infections ◽  
Pathogenic Role ◽  
Infected Wounds ◽  
The Tropics ◽  
Nose And Throat

A bacteriological survey was undertaken on clinically infected traumatic wounds amongst a group of young and fit Operation Raleigh members, who were living and working in a remote area of Costa Rican rain forest. All infected wounds were swabbed before treatment and, where possible, at intervals during treatment. Swabs were also obtained from the nose and throat of each patient. All swabs were stored by desiccation in sterile silica gel for culture at a later date. Culture revealed a high rate of isolation of Bacillus cereus from the wounds. The organism was commonly isolated in pure and heavy growth. Contamination by B. cereus was considered and excluded experimentally. Preliminary toxological studies have shown that the majority of the isolates produce a necrotic exotoxin, in keeping with the clinical findings. These results suggest that B. cereus caused significant sepsis in this series of traumatic wounds.

scholarly journals Enzymatically-crosslinked gelatin hydrogels containing paenipeptin and clarithromycin against carbapenem-resistant pathogen in murine skin wound infection

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Sun Hee Moon ◽  
Yihong Kaufmann ◽  
Ryoichi Fujiwara ◽  
En Huang
Keyword(s):  
Wound Infection ◽  
Multidrug Resistant ◽  
Skin Wound ◽  
Drug Resistant ◽  
Wound Infections ◽  
Log Reduction ◽  
Carbapenem Resistant ◽  
Infected Wounds ◽  
Localized Delivery

Abstract Background The recent rise and spread of carbapenem-resistant pathogens pose an urgent threat to public health and has fueled the search for new therapies. Localized delivery of topical antibiotics is an alternative for the treatment of infected wounds caused by drug-resistant pathogens. In this study, we aimed to develop antimicrobial-loaded hydrogels for topical treatment of wound infections in a murine skin wound infection. Results Paenipeptin analogue 1, a linear lipopeptide, potentiated clarithromycin against multidrug-resistant Acinetobacter baumannii, Enterobacter cloacae, Escherichia coli, and Klebsiella pneumoniae. Enzymatically-crosslinked gelatin hydrogels were developed to encapsulate paenipeptin analogue 1 and clarithromycin. The encapsulated antimicrobials were gradually released from hydrogels during incubation, reaching 75.43 and 53.66% for paenipeptin and clarithromycin, respectively, at 24 h. The antimicrobial-loaded hydrogels containing paenipeptin and clarithromycin synergistically resulted in 5-log reduction in carbapenem-resistant A. baumannii within 6 h in vitro. Moreover, the antimicrobial-loaded hydrogels reduced 3.6- and 2.5-log of carbapenem-resistant A. baumannii when treated at 4 or 20 h post infection, respectively, in a murine skin wound infection. Conclusions Enzymatically-crosslinked gelatin hydrogels loaded with paenipeptin analogue 1 and clarithromycin exhibited potent therapeutic efficacy against carbapenem-resistant A. baumannii in murine skin wound infection.

scholarly journals Studying the surface-tethered AaaA as a virulence factor and anti-P. aeruginosa drug target

2020 ◽  
Vol 2 (7A) ◽  
Author(s):  
Claire S. Laxton ◽  
Elena Jordana-Lluch ◽  
Jeni Luckett ◽  
Stephan Heeb ◽  
Kim Hardie
Keyword(s):  
Virulence Factor ◽  
Laser Scanning ◽  
Chronic Wounds ◽  
Immune Cell ◽  
Ex Vivo ◽  
Skin Wound ◽  
Laser Scanning Microscopy ◽  
Wound Infections ◽  
Immune Factor

P. aeruginosa is a leading cause of bacterial wound infections, and is associated with a disproportionally high level of mortality in burn patients especially. Because of its wide arsenal of virulence factors and biofilm formation ability, infections with P. aeruginosa are often chronic and extremely difficult to treat. This study uses an ex-vivo skin model to study the role of the virulence factor AaaA (PA0328) in chronic wound infections. AaaA, or the Arginine-specific aminopeptidase of Pseudomonas aeruginosa A, is a surface-tethered autotransporter which cleaves N-terminal arginine from peptides. In the oxygen and nutrient-limited environments of chronic wounds, this free arginine could serve as a nutrient source for P. aeruginosa via alternative metabolic pathways. Changes in local arginine concentrations may also alter host iNOS and Arginase immune responses which influence inflammation and wound healing, in order to favour a chronic infection. Being surface-tethered and immunogenic, AaaA is also of interest as a potential antimicrobial drug or vaccine target. This study aims to probe the role of AaaA on both pathogen survival and host response in the wound context, using a combination of in situ transcriptional reporters, immunofluorescence and laser-scanning microscopy, and RT-qPCR to localise and quantify P. aeruginosa survival and aaaA expression, as well as resident immune cell invasion, and expression of immune factor genes, in wounds over time. Here, we present preliminary data examining AaaA expression and function in P. aeruginosa biofilm cultures and ex-vivo skin wound infections, as well as the results of preliminary inhibitor screens.

scholarly journals Bacillus cereus wound infections.

1979 ◽  
Vol 32 (12) ◽  
pp. 1305-1305 ◽  
Author(s):  
H J Andrews ◽  
D M Evans
Keyword(s):  
Bacillus Cereus ◽  
Wound Infections

Antibiogram of bacterial species causing skin wound infections

2018 ◽  
Vol 2 (3) ◽  
pp. 53-60
Author(s):  
Mahesh Pant ◽  
Dipti Shrestha ◽  
Shovana Thapa
Keyword(s):  
Bacterial Species ◽  
Skin Wound ◽  
Wound Infections

scholarly journals Successful Treatment ofBacillus cereusBacteremia in a Patient with Propionic Acidemia

2016 ◽  
Vol 2016 ◽  
pp. 1-2
Author(s):  
Fatma Deniz Aygun ◽  
Fatih Aygun ◽  
Halit Cam
Keyword(s):  
Bacillus Cereus ◽  
Severe Disease ◽  
Bloodstream Infections ◽  
Propionic Acidemia ◽  
Immunocompromised Patients ◽  
Wound Infections ◽  
Intravascular Catheter ◽  
Life Threatening ◽  
Traumatic Wound ◽  
Systemic Infections

Bacillus cereuscan cause serious, life-threatening, systemic infections in immunocompromised patients. The ability of microorganism to form biofilm on biomedical devices can be responsible for catheter-related bloodstream infections. Other manifestations of severe disease are meningitis, endocarditis, osteomyelitis, and surgical and traumatic wound infections. The most common feature in true bacteremia caused by Bacillus is the presence of an intravascular catheter. Herein, we report a case of catheter-related bacteremia caused byB. cereusin a patient with propionic acidemia.

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