Evaluation of scaffolding, inflammatory response, and wound healing support of a reverse thermal gel for myelomeningocele patching

There is growing interest in expanding the clinical applications for HBO2(hyperbaric oxygen therapy) into new medical and surgical fields. The pathophysiology of response towards wounds, infection, trauma, or surgery involves various chemical mediators that include cytokines, prostaglandins (PGs), and nitric oxide (NO). The beneficial role played by HBO2in wound healing, carbon monoxide poisoning, decompression sickness, and other indications is well documented. However, the exact mechanism of action is still poorly understood. This review addresses the effects of HBO2on PGs, NO, and cytokines involved in wound pathophysiology and inflammation in particular. The results of this review indicate that HBO2has important effects on the biology of cytokines and other mediators of inflammation. HBO2causes cytokine down-regulation and growth factor up-regulation. HBO2transiently suppresses stimulus-induced proinflammatory cytokine production and affects the liberation of TNFα (tumor necrosis factor alpha) and endothelins. VEGF (vascular endothelial growth factor) levels are significantly increased with HBO2, whereas the value of PGE2 and COX-2 mRNA are markedly reduced. The effect of HBO2on NO production is not well established and more studies are required. In conclusion, cytokines, PGs, and NO may play a major role in the mechanism of action of HBO2 and further research could pave the way for new clinical applications for HBO2to be established. It could be proposed that chronic wounds persist due to an uncontrolled pathological inflammatory response in the wound bed and that HBO2enhances wound healing by damping pathological inflammation (anti-inflammatory effects); this hypothetical proposal remains to be substantiated with experimental results.

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Oxandrolone protects against the development of multiorgan failure, modulates the systemic inflammatory response and promotes wound healing during burn injury

Burns ◽

10.1016/j.burns.2018.10.006 ◽

2019 ◽

Vol 45 (3) ◽

pp. 671-681 ◽

Cited By ~ 4

Author(s):

Akbar Ahmad ◽

David N. Herndon ◽

Csaba Szabo

Keyword(s):

Wound Healing ◽

Inflammatory Response ◽

Burn Injury ◽

Multiorgan Failure ◽

Systemic Inflammatory Response

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Investigating the Wound Healing Potential of the Polyherbal Gels Prepared with Ficus Extract

International Journal of Novel Trends in Pharmaceutical Sciences ◽

10.26452/ijntps.v8i2.1313 ◽

2018 ◽

Vol 8 (2) ◽

pp. 13-16

Author(s):

Mahender K ◽

Ravi D ◽

Chaitanya Kumar K ◽

Mothilal K

Keyword(s):

Wound Healing ◽

Inflammatory Response ◽

Human Body ◽

Healing Process ◽

The Body ◽

Wound Healing Process ◽

Aqueous Extracts ◽

Standard Drug ◽

Carbopol Gel ◽

Ficus Benghalensis

Wounds are nothing but any damage to the tissue or skin that can be healed. The wound healing process is usually built in the human body to self heal many wounds. When there is an injury in the body, there is an inflammatory response that is generated in the body, and the cells begin to raise the collagen levels in the skin which enables to increase the healing process. Ficus species of plants are famous for their potency to treat diseases in various Indian systems of medicine and the tree is commonly called as a banyan. Especially the plant in the species benghalensis is used to treat rheumatism, wounds and other skin related problems like an ulcer. The herbal gels were prepared using the incorporation of the aqueous extracts of the plant Ficus benghalensis into carbopol gel. They were investigated for the wound healing potential compared to the betadine drug standard. The gels at a concentration 200mg/g of the gel showed better activity compared to the gel at 100mg/g and the standard drug, betadine.

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A 3D In Vitro Model for Burn Wounds: Monitoring of Regeneration on the Epidermal Level

Biomedicines ◽

10.3390/biomedicines9091153 ◽

2021 ◽

Vol 9 (9) ◽

pp. 1153

Author(s):

Verena Schneider ◽

Daniel Kruse ◽

Ives Bernardelli de Mattos ◽

Saskia Zöphel ◽

Kendra-Kathrin Tiltmann ◽

...

Keyword(s):

Wound Healing ◽

Inflammatory Response ◽

Healing Process ◽

Three Dimensional ◽

Source Model ◽

Burn Wound ◽

Thermal Burn ◽

Burn Wounds

Burns affect millions every year and a model to mimic the pathophysiology of such injuries in detail is required to better understand regeneration. The current gold standard for studying burn wounds are animal models, which are under criticism due to ethical considerations and a limited predictiveness. Here, we present a three-dimensional burn model, based on an open-source model, to monitor wound healing on the epidermal level. Skin equivalents were burned, using a preheated metal cylinder. The healing process was monitored regarding histomorphology, metabolic changes, inflammatory response and reepithelialization for 14 days. During this time, the wound size decreased from 25% to 5% of the model area and the inflammatory response (IL-1β, IL-6 and IL-8) showed a comparable course to wounding and healing in vivo. Additionally, the topical application of 5% dexpanthenol enhanced tissue morphology and the number of proliferative keratinocytes in the newly formed epidermis, but did not influence the overall reepithelialization rate. In summary, the model showed a comparable healing process to in vivo, and thus, offers the opportunity to better understand the physiology of thermal burn wound healing on the keratinocyte level.

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The Effect of 17β-Estradiol Administration on Cutaneous Wound Healing in 24-Week Ovariectomized Female Mice

Journal of Hormones ◽

10.1155/2014/234632 ◽

2014 ◽

Vol 2014 ◽

pp. 1-8 ◽

Cited By ~ 2

Author(s):

Kanae Mukai ◽

Yukari Nakajima ◽

Tamae Urai ◽

Emi Komatsu ◽

Kana Takata ◽

...

Keyword(s):

Wound Healing ◽

Inflammatory Response ◽

Cutaneous Wound Healing ◽

Full Thickness ◽

Estrogen Replacement ◽

Wound Area ◽

Cutaneous Wound ◽

Female Mice ◽

Exogenous Estrogen ◽

17Β Estradiol

Estrogen replacement promotes cutaneous wound healing in 8–10-week young ovariectomized female mice. However, research using aged ovariectomized female mice has not been reported, to the best of our knowledge. Therefore, we investigated the effect of 17β-estradiol on cutaneous wound healing using 24-week middle-aged ovariectomized female mice. Twenty-week-old female mice were divided into three groups: medication with 17β-estradiol after ovariectomy (OVX + 17β-estradiol), ovariectomy (OVX), and sham (SHAM). After 4 weeks, the mice received two full-thickness wounds. Then, the OVX + 17β-estradiol group was administered 17β-estradiol at 0.01 g/day until healing. The ratio of wound area in the OVX + 17β-estradiol group was significantly decreased compared with that in the OVX group. The numbers of neutrophils and macrophages in the OVX + 17β-estradiol group were significantly smaller than those in the OVX group. In addition, the ratio of myofibroblasts in the OVX + 17β-estradiol group was significantly higher than that in the OVX group. These data suggested that exogenous continuous 17β-estradiol administration promotes cutaneous wound healing in 24-week OVX female mice by reducing wound area, shortening inflammatory response, and promoting wound contraction. However, it is unclear whether the effect of exogenous estrogen on wound healing outweighs the delay of wound healing due to advanced age.

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miR-19a/b and miR-20a Promote Wound Healing by Regulating the Inflammatory Response of Keratinocytes

Journal of Investigative Dermatology ◽

10.1016/j.jid.2020.06.037 ◽

2020 ◽

Cited By ~ 2

Author(s):

Dongqing Li ◽

Hongmei Peng ◽

Le Qu ◽

Pehr Sommar ◽

Aoxue Wang ◽

...

Keyword(s):

Wound Healing ◽

Inflammatory Response ◽

Promote Wound Healing

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Deregulated immune cell recruitment orchestrated by FOXM1 impairs human diabetic wound healing

Nature Communications ◽

10.1038/s41467-020-18276-0 ◽

2020 ◽

Vol 11 (1) ◽

Author(s):

Andrew P. Sawaya ◽

Rivka C. Stone ◽

Stephen R. Brooks ◽

Irena Pastar ◽

Ivan Jozic ◽

...

Keyword(s):

Wound Healing ◽

Inflammatory Response ◽

Molecular Mechanisms ◽

Immune Cell ◽

Diabetic Mouse ◽

Transcriptional Networks ◽

Life Threatening ◽

Diabetic Wound Healing ◽

Diabetic Wounds

Abstract Diabetic foot ulcers (DFUs) are a life-threatening disease that often result in lower limb amputations and a shortened lifespan. However, molecular mechanisms contributing to the pathogenesis of DFUs remain poorly understood. We use next-generation sequencing to generate a human dataset of pathogenic DFUs to compare to transcriptional profiles of human skin and oral acute wounds, oral as a model of “ideal” adult tissue repair due to accelerated closure without scarring. Here we identify major transcriptional networks deregulated in DFUs that result in decreased neutrophils and macrophages recruitment and overall poorly controlled inflammatory response. Transcription factors FOXM1 and STAT3, which function to activate and promote survival of immune cells, are inhibited in DFUs. Moreover, inhibition of FOXM1 in diabetic mouse models (STZ-induced and db/db) results in delayed wound healing and decreased neutrophil and macrophage recruitment in diabetic wounds in vivo. Our data underscore the role of a perturbed, ineffective inflammatory response as a major contributor to the pathogenesis of DFUs, which is facilitated by FOXM1-mediated deregulation of recruitment of neutrophils and macrophages, revealing a potential therapeutic strategy.

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