scholarly journals Expression system of CotA-laccase for directed evolution and high-throughput screenings for the oxidation of high-redox potential dyes

2009 ◽  
Vol 4 (4) ◽  
pp. 558-563 ◽  
Author(s):  
Vânia Brissos ◽  
Luciana Pereira ◽  
Florentina-Daniela Munteanu ◽  
Artur Cavaco-Paulo ◽  
Lígia O. Martins
Keyword(s):  
Redox Potential ◽  
Directed Evolution ◽  
High Throughput ◽  
Expression System ◽  
Cota Laccase ◽  
High Redox Potential

scholarly journals CotA laccase: high-throughput manipulation and analysis of recombinant enzyme libraries expressed in E. coli using droplet-based microfluidics

The Analyst ◽  
2014 ◽  
Vol 139 (13) ◽  
pp. 3314-3323 ◽  
Author(s):  
Thomas Beneyton ◽  
Faith Coldren ◽  
Jean-Christophe Baret ◽  
Andrew D. Griffiths ◽  
Valérie Taly
Keyword(s):  
Directed Evolution ◽  
High Throughput ◽  
Recombinant Enzyme ◽  
Cell Analysis ◽  
E Coli ◽  
Cota Laccase

A high-throughput cell analysis and sorting platform using droplet-based microfluidics is introduced for directed evolution of recombinant CotA laccase expressed in E. coli.

scholarly journals Switching from blue to yellow: altering the spectral properties of a high redox potential laccase by directed evolution

2012 ◽  
Vol 31 (1) ◽  
pp. 8-21 ◽  
Author(s):  
Diana M. Mate ◽  
Eva Garcia-Ruiz ◽  
Susana Camarero ◽  
Vladimir V. Shubin ◽  
Magnus Falk ◽  
...  
Keyword(s):  
Redox Potential ◽  
Directed Evolution ◽  
Spectral Properties ◽  
High Redox Potential

scholarly journals Electrochemistry of a high redox potential laccase obtained by computer-guided mutagenesis combined with directed evolution

2019 ◽  
Vol 106 ◽  
pp. 106511 ◽  
Author(s):  
Olga Aleksejeva ◽  
Ivan Mateljak ◽  
Roland Ludwig ◽  
Miguel Alcalde ◽  
Sergey Shleev
Keyword(s):  
Redox Potential ◽  
Directed Evolution ◽  
High Redox Potential

Directed Evolution of P450 Fatty Acid Decarboxylases via High-Throughput Screening Towards Improved Catalytic Activity

2019 ◽  
Author(s):  
Huifang Xu ◽  
Weinan Liang ◽  
Linlin Ning ◽  
Yuanyuan Jiang ◽  
Wenxia Yang ◽  
...  
Keyword(s):  
Catalytic Activity ◽  
Fatty Acid ◽  
Directed Evolution ◽  
High Throughput ◽  
High Throughput Screening ◽  
Colorimetric Detection ◽  
Screening Method ◽  
Random Mutagenesis ◽  
Direct Production ◽  
Consumption Activity

P450 fatty acid decarboxylases (FADCs) have recently been attracting considerable attention owing to their one-step direct production of industrially important 1-alkenes from biologically abundant feedstock free fatty acids under mild conditions. However, attempts to improve the catalytic activity of FADCs have met with little success. Protein engineering has been limited to selected residues and small mutant libraries due to lack of an effective high-throughput screening (HTS) method. Here, we devise a catalase-deficient <i>Escherichia coli</i> host strain and report an HTS approach based on colorimetric detection of H<sub>2</sub>O<sub>2</sub>-consumption activity of FADCs. Directed evolution enabled by this method has led to effective identification for the first time of improved FADC variants for medium-chain 1-alkene production from both DNA shuffling and random mutagenesis libraries. Advantageously, this screening method can be extended to other enzymes that stoichiometrically utilize H<sub>2</sub>O<sub>2</sub> as co-substrate.

A few key residues determine the high redox potential shift in azurin mutants

2015 ◽  
Vol 13 (45) ◽  
pp. 11003-11013 ◽  
Author(s):  
Laura Zanetti-Polzi ◽  
Carlo A. Bortolotti ◽  
Isabella Daidone ◽  
Massimiliano Aschi ◽  
Andrea Amadei ◽  
...  
Keyword(s):  
Redox Potential ◽  
Potential Shift ◽  
Key Residues ◽  
High Redox Potential

The changes in the redox potential of Azurin upon mutation stem from the effects of a few key residues, including non-mutated ones, rather than being the result of a generalized rearrangement.

Fluorescence Activated Cell Sorting as a General Ultra-High-Throughput Screening Method for Directed Evolution of Glycosyltransferases

2010 ◽  
Vol 132 (30) ◽  
pp. 10570-10577 ◽  
Author(s):  
Guangyu Yang ◽  
Jamie R. Rich ◽  
Michel Gilbert ◽  
Warren W. Wakarchuk ◽  
Yan Feng ◽  
...  
Keyword(s):  
Directed Evolution ◽  
High Throughput ◽  
Cell Sorting ◽  
High Throughput Screening ◽  
Screening Method ◽  
Fluorescence Activated Cell Sorting

Electronic Characterization of the Oxidized State of the Blue Copper Protein Rusticyanin by1H NMR:  Is the Axial Methionine the Dominant Influence for the High Redox Potential?†

Biochemistry ◽  
2001 ◽  
Vol 40 (3) ◽  
pp. 837-846 ◽  
Author(s):  
Antonio Donaire ◽  
Beatriz Jiménez ◽  
José-María Moratal ◽  
John F. Hall ◽  
S. Samar Hasnain
Keyword(s):  
Redox Potential ◽  
Blue Copper Protein ◽  
Blue Copper ◽  
Copper Protein ◽  
Dominant Influence ◽  
High Redox Potential

scholarly journals Purification and Partial Characterization of a Thermostable Laccase from Pycnoporus sanguineus CS-2 with Ability to Oxidize High Redox Potential Substrates and Recalcitrant Dyes

10.5772/56374 ◽  
2013 ◽  
Author(s):  
Sergio M. Salcedo Martnez ◽  
Guadalupe Gutirrez-Soto ◽  
Carlos F. Rodriguez Garza ◽  
Tania J. Villarreal Galvan ◽  
Juan F. Contreras Cordero ◽  
...  
Keyword(s):  
Redox Potential ◽  
Partial Characterization ◽  
Pycnoporus Sanguineus ◽  
High Redox Potential
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