Volatile fatty acids derived from waste organics provide an economical carbon source for microbial lipids/biodiesel production

Microbial lipid production from oleaginous yeasts is a promising process for the sustainable development of the microbial biodiesel industry. However, the feedstock cost poses an economic problem for the production of microbial biodiesel. After lipid extraction, yeast biomass can be used as an organic source for microbial biodiesel production. In this study, volatile fatty acids (VFAs), produced via anaerobic digestion of a lipid-extracted yeast (LEY) residue, were utilized as a carbon source for the yeast Cryptococcus curvatus. The response surface methodology was used to determine the initial pH and inoculum volume for the optimal VFA production. The experimental result for VFA concentration was 4.51 g/L at an initial pH of 9 and an inoculation 25%. The optimization results from the response surface methodology showed that the maximal VFA concentration was 4.58 g/L at an initial pH of 8.40 and an inoculation of 39.49%. This study indicates that VFAs from LEY can be used as a carbon source for microbial biodiesel production, with the potential to significantly reduce feedstock costs.

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The effect of volatile fatty acids as a sole carbon source on lipid accumulation by Cryptococcus albidus for biodiesel production

Bioresource Technology ◽

10.1016/j.biortech.2010.10.141 ◽

2011 ◽

Vol 102 (3) ◽

pp. 2695-2701 ◽

Cited By ~ 172

Author(s):

Qiang Fei ◽

Ho Nam Chang ◽

Longan Shang ◽

Jin-dal-rae Choi ◽

NagJong Kim ◽

...

Keyword(s):

Fatty Acids ◽

Carbon Source ◽

Lipid Accumulation ◽

Sole Carbon Source ◽

Volatile Fatty Acids ◽

Biodiesel Production ◽

Cryptococcus Albidus

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Production of microbial lipids utilizing volatile fatty acids derived from wastepaper: A biorefinery approach for biodiesel production

Fuel ◽

10.1016/j.fuel.2020.118087 ◽

2020 ◽

Vol 276 ◽

pp. 118087 ◽

Cited By ~ 3

Author(s):

Neelamegam Annamalai ◽

Nallusamy Sivakumar ◽

Alfred Fernandez-Castane ◽

Piotr Oleskowicz-Popiel

Keyword(s):

Fatty Acids ◽

Volatile Fatty Acids ◽

Biodiesel Production ◽

Microbial Lipids

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Recovery and Utilization of Volatile Fatty Acids from Faecal Sludge for in-situ Pathogen Reduction and Biodiesel Production through Microbial Lipid Synthesis

Proceedings of the Water Environment Federation ◽

10.2175/193864715819538868 ◽

2015 ◽

Vol 2015 (19) ◽

pp. 5928-5929

Author(s):

Shashwat Vajpeyi ◽

Kartik Chandran

Keyword(s):

Fatty Acids ◽

Volatile Fatty Acids ◽

Lipid Synthesis ◽

Biodiesel Production ◽

Microbial Lipid ◽

Pathogen Reduction ◽

Faecal Sludge

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Producing Omega-3 Polyunsaturated Fatty Acids: A Review of Sustainable Sources and Future Trends for the EPA and DHA Market

Resources ◽

10.3390/resources9120148 ◽

2020 ◽

Vol 9 (12) ◽

pp. 148

Author(s):

Laura Oliver ◽

Thomas Dietrich ◽

Izaskun Marañón ◽

Maria Carmen Villarán ◽

Ramón J. Barrio

Keyword(s):

Fatty Acids ◽

Carbon Source ◽

Polyunsaturated Fatty Acids ◽

Volatile Fatty Acids ◽

Good Alternative ◽

Future Market ◽

Omega 3 ◽

Alternative Pathways ◽

Healthy Nutrition ◽

Main Challenge

Omega-3 polyunsaturated fatty acids (Omega-3 PUFA) are recognized as being essential compounds for human nutrition and health. The human body generates only low levels of Omega-3 PUFA. Conventional sources of Omega-3 PUFA are from marine origin. However, the global growth of population combined with a better consumer understanding about healthy nutrition leads to the fact that traditional sources are exhausted and therefore not enough to satisfy the demand of Omega-3 PUFA for human diet as well as aquaculture. Microalgae cultivated under heterotrophic conditions is increasingly recognized as a suitable technology for the production of the Omega-3 PUFA. The high cost of using glucose as main carbon source for cultivation is the main challenge to establish economical feasible production processes. The latest relevant studies provide alternative pathways for Omega-3 PUFA production. As preliminary results show, volatile fatty acids (VFA) recovered from waste stream could be a good alternative to the use of glucose as carbon source in microalgae cultivation. The purpose of this paper is to highlight the actual situation of Omega-3 PUFA production, sources and market request to provide a summary on sustainable sources that are being investigated as well as present and future market trends in Omega-3 market.

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Efficient bioconversion of high-content volatile fatty acids into microbial lipids by Cryptococcus curvatus ATCC 20509

Bioresource Technology ◽

10.1016/j.biortech.2017.04.070 ◽

2017 ◽

Vol 239 ◽

pp. 394-401 ◽

Cited By ~ 20

Author(s):

Jia'nan Liu ◽

Xiangfeng Huang ◽

Rui Chen ◽

Ming Yuan ◽

Jia Liu

Keyword(s):

Fatty Acids ◽

Volatile Fatty Acids ◽

Microbial Lipids ◽

Cryptococcus Curvatus

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Pengaruh sumber karbon terhadap produksi bioplastik polihidroksialkanoat (PHA) dengan ralstonia eutropha

Jurnal Teknik Kimia Indonesia ◽

10.5614/jtki.2010.9.1.4 ◽

2018 ◽

Vol 9 (1) ◽

pp. 28

Author(s):

Martha Aznury ◽

Tjandra Setiadi ◽

Adi Pancoro

Keyword(s):

Fatty Acids ◽

Fatty Acid ◽

Carbon Source ◽

Fermentation Process ◽

Volatile Fatty Acids ◽

Ralstonia Eutropha ◽

Operating Conditions ◽

Batch Bioreactor ◽

Pha Production ◽

Fructose Fermentation

Bioplastic Polyhidroxyalknoate (PHA) is a polyester type bioplastic with physicochemical properties resemble to those of polypropilen from petroleum. PHA production was investigated to determine the effect of carbon source on the fermentation process by Ralstonia eutropha. Specifically, Ralstonia eutropha was cultivated in a batch bioreactor to show the dynamics of P(3HB-co-3HV) copolymer production from glucose or fructose as C source. In adition, the effect of volatile fatty acids addition, as stimulator to the copolymer production, was also studied. The operating conditions in a 7 L bioreactor were at temperature 30 oC and pH 7.0. The concentration of carbon source glucose or fructose was 40 g/L, and after 20 hour fermentation, volatile fatty acids were added. With volatile fatty acids addition, the resulting fructose fermentation had PHA content of 32.78%, in which the HV percentage was 11.78%. Meanwhile, the fermentation of glucose, stimulated by volatile fatty acids, gave PHA as much as 20.19% with HV percentage of 8.71%. Therefore,, the Ralstonia eutropha fermentation of fructose as the carbon source gave a higher yield than glucose. Keywords: Volatil Fatty Acid, Fructose, Glucose, PHA, P(3HB-co-3HV), Ralstonia eutropha AbstrakBioplastik polihidroksialkanoat (PHA) adalah bioplastik dari kelompok poliester dengan sifat fisikokimia mirip dengan plastik polipropilen dari minyak bumi. Penelitian ini bertujuan untuk mempelajari pengaruh sumber karbon terhadap poduksi PHA yang dilakukan dengan proses fermentasi menggunakan Ralstonia eutropha. Ralstonia eutropha dikultivasi dalam bioreaktor batch untuk mempelajari dinamika produksi kopolimer P(3HB-co-3HV) dari sumber karbon glukosa atau fruktosa, serta mempelajari pengaruh sumber stimulator asam lemak volatil. Kondisi operasional fermentasi menggunakan bioreaktor 7 L adalah pada temperatur 30 oC dan pH 7. Konsentrasi sumber karbon glukosa atau fruktosa yang digunakan adalah 40 gr/L, dan setelah 20 jam fermentasi ditambahkan asam lemak volatil yang berfungsi sebagai stimulator dalam produksi P(3HB-co-3HV). Panen sel Ralstonia eutropha dilakukan setelah 60 jam. Hasil penelitian menunjukkan fermentasi Ralstonia eutropha dengan substrat fruktosa dan asam lemak volatil sebagai stimulator mempunyai kandungan PHA sebesar 32,78%, dengan kadar HV 11,78%. Pada pemberian substrat glukosa dan asam lemak volatil menunjukkan kandungan PHA sebesar 20,19%, dengan kadar HV 8.71%. Jadi fermentasi Ralstonia eutropha dengan menggunakan substrat fruktosa memberikan yield yang lebih tinggi dibandingkan menggunakan substrat glukosa.Kata Kunci: Asam lemak volatil, fruktosa, glukosa, PHA, P(3HB-co-3HV), Ralstonia eutropha

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