scholarly journals Purification of cell culture-derived human influenza A virus by size-exclusion and anion-exchange chromatography

2007 ◽  
Vol 96 (5) ◽  
pp. 932-944 ◽  
Author(s):  
Bernd Kalbfuss ◽  
Michael Wolff ◽  
Robert Morenweiser ◽  
Udo Reichl
Keyword(s):  
Cell Culture ◽  
Anion Exchange ◽  
Influenza A Virus ◽  
Influenza A ◽  
Anion Exchange Chromatography ◽  
Exchange Chromatography ◽  
Size Exclusion ◽  
Human Influenza

Analysis of the substituent distribution along the chain of water-soluble methyl cellulose by combination of enzymatic and chemical methods

Holzforschung ◽  
2004 ◽  
Vol 58 (1) ◽  
pp. 97-104 ◽  
Author(s):  
B. Saake ◽  
S. Lebioda ◽  
J. Puls
Keyword(s):  
Anion Exchange ◽  
Size Exclusion Chromatography ◽  
Methyl Cellulose ◽  
Anion Exchange Chromatography ◽  
Exchange Chromatography ◽  
Degree Of Substitution ◽  
Water Soluble ◽  
Size Exclusion ◽  
Exclusion Chromatography ◽  
C Nmr

Abstract Four methyl cellulose samples in the degree of substitution range from 0.5 to 2.0 were characterised by combination of different analytical methods. Samples were analysed regarding their partial degree of substitution by hydrolysis and anion exchange chromatography with pulsed amperometric detection. For calibration of the chromatographic system, standard substances were isolated by preparative HPLC and their structure was confirmed by 13C-NMR spectroscopy. For two methyl cellulose samples per-acetylation and 13C-NMR with inverse gated decoupling was carried out for comparison with the chromatographic analysis. Endoglucanase fragmentation of methyl celluloses was performed and water-soluble and insoluble fractions were analysed separately. A preparative size exclusion chromatography system for enzymatic-degraded water-soluble methyl cellulose was developed and the molar masses of the individual fractions were examined by analytical size exclusion chromatography. By combination of endoglucanase fragmentation, preparative chromatography, hydrolysis and anion exchange chromatography an approach for the analysis of the substitutent distribution along the polymeric chain of water-soluble methyl cellulose could be established.

Bioprospection of Antiviral and Antitumor Compounds from Some Marine Algae from Egyptian Shoers

2021 ◽  
Vol 21 ◽  
Author(s):  
Essam M. Ahmed ◽  
Abdelhamid A. Hamdy ◽  
Bandar M. Alshehri
Keyword(s):  
Liquid Chromatography ◽  
Anion Exchange ◽  
Marine Algae ◽  
Anion Exchange Chromatography ◽  
Exchange Chromatography ◽  
Size Exclusion ◽  
Active Compounds ◽  
Ethanolic Extracts ◽  
Exclusion Chromatography ◽  
Antiviral Activities

Background: The marine algae are considered a diverse source of bioactive compounds. Many active compounds have been isolated from algae and show good biological activities. Materials and Methods: The aim of this study is to detect the antiviral and anticancer activities in some extracts of marine algae. Extraction, purification and identification of some marine algae common in Egypt were conducted. Extraction of Ulva lactuca, Sargassum dentifolium, and Cystoseiara myrica was conducted. A sequence of extractions, including extraction by ethanol, boiling water, hydrochloric acid and sodium hydroxide were carried out. The obtained extracts were evaluated for their antitumour and antiviral activities against liver tumour cells, brain tumour cell lines, measles virus, mumps virus and hepatitis B virus (HBV). The extracts of the best activities were subjected for purification by size exclusion chromatography and anion exchange chromatography for ethanolic extracts or precipitation by cetylpyridinium chloride (CPC) then by size exclusion chromatography and anion exchange chromatography for aqueous extracts. Separation by GLS/MS was performed. The structures of the active compounds have been identified through different chemical analyses, including sugar analysis, configurational analysis, high-performance liquid chromatography (HPLC), infrared spectroscopy (IR), gas-liquid chromatography-mass spectroscopy (GLC-MS) and 1H,13C nuclear magnetic resonance (NMR) at ZV. Results: The active compounds from the water extracts have been identified mainly as polysaccharides and sulphated polysaccharides. The antitumour and the antiviral activities of ethanolic extracts are attributable to compound identified as Ethyl Palmitate. These natural compounds did not show cytotoxic effect. Conclusion: These outputs could be preliminary for further biological studies aiming to therapeutic application.

scholarly journals Caveolin-1 influences human influenza A virus (H1N1) multiplication in cell culture

2010 ◽  
Vol 7 (1) ◽  
pp. 108 ◽  
Author(s):  
Lijing Sun ◽  
Gun-Viol Hemgård ◽  
Sony A Susanto ◽  
Manfred Wirth
Keyword(s):  
Cell Culture ◽  
Influenza A Virus ◽  
Influenza A ◽  
Virus H1n1 ◽  
Human Influenza ◽  
Caveolin 1

Harvesting and concentration of human influenza A virus produced in serum-free mammalian cell culture for the production of vaccines

2007 ◽  
Vol 97 (1) ◽  
pp. 73-85 ◽  
Author(s):  
Bernd Kalbfuss ◽  
Yvonne Genzel ◽  
Michael Wolff ◽  
Anke Zimmermann ◽  
Robert Morenweiser ◽  
...  
Keyword(s):  
Cell Culture ◽  
Influenza A Virus ◽  
Mammalian Cell ◽  
Influenza A ◽  
Mammalian Cell Culture ◽  
Human Influenza ◽  
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