Design and analysis of a long-term live-cell imaging chamber for tracking cellular dynamics within cultured human islets of Langerhans

2007 ◽  
Vol 97 (5) ◽  
pp. 1138-1147 ◽  
Author(s):  
Duane Moogk ◽  
Stephen Hanley ◽  
John Ramunas ◽  
April Blaylock ◽  
Jana Skorepova ◽  
...  
Keyword(s):  
Islets Of Langerhans ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Human Islets ◽  
Live Cell ◽  
Cellular Dynamics ◽  
Human Islets Of Langerhans

Long-term cryogenic storage of purified adult human islets of Langerhans

Diabetes ◽  
1989 ◽  
Vol 38 (3) ◽  
pp. 386-396 ◽  
Author(s):  
N. M. Kneteman ◽  
D. Alderson ◽  
D. W. Scharp ◽  
P. E. Lacy
Keyword(s):  
Islets Of Langerhans ◽  
Human Islets ◽  
Cryogenic Storage ◽  
Adult Human ◽  
Human Islets Of Langerhans

pH-triggered decomposition of polymeric fluorescent vesicles to induce growth of tetraphenylethylene nanoparticles for long-term live cell imaging

Polymer ◽  
2017 ◽  
Vol 118 ◽  
pp. 75-84 ◽  
Author(s):  
Xing Wang ◽  
Yanyu Yang ◽  
Fei Yang ◽  
Hong Shen ◽  
Decheng Wu
Keyword(s):  
Live Cell Imaging ◽  
Cell Imaging ◽  
Live Cell

Long-Term Cryogenic Storage of Purified Adult Human Islets of Langerhans

Diabetes ◽  
1989 ◽  
Vol 38 (3) ◽  
pp. 386-396 ◽  
Author(s):  
N. M. Kneteman ◽  
D. Alderson ◽  
D. W. Scharp ◽  
P. E. Lacy
Keyword(s):  
Islets Of Langerhans ◽  
Human Islets ◽  
Cryogenic Storage ◽  
Adult Human ◽  
Human Islets Of Langerhans

scholarly journals Long-term fate of etoposide-induced micronuclei and micronucleated cells in Hela-H2B-GFP cells

2020 ◽  
Vol 94 (10) ◽  
pp. 3553-3561
Author(s):  
Hauke Reimann ◽  
Helga Stopper ◽  
Henning Hintzsche
Keyword(s):  
Cell Cycle ◽  
Cell Death ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Live Cell ◽  
Cellular Structures ◽  
Death Rates ◽  
Daughter Cells

Abstract Micronuclei are small nuclear cellular structures containing whole chromosomes or chromosomal fragments. While there is a lot of information available about the origin and formation of micronuclei, less is known about the fate of micronuclei and micronucleated cells. Possible fates include extrusion, degradation, reincorporation and persistence. Live cell imaging was performed to quantitatively analyse the fates of micronuclei and micronucleated cells occurring in vitro. Imaging was conducted for up to 96 h in HeLa-H2B-GFP cells treated with 0.5, 1 and 2 µg/ml etoposide. While a minority of micronuclei was reincorporated into the main nucleus during mitosis, the majority of micronuclei persisted without any alterations. Degradation and extrusion were observed rarely or never. The presence of micronuclei affected the proliferation of the daughter cells and also had an influence on cell death rates. Mitotic errors were found to be clearly increased in micronucleus-containing cells. The results show that micronuclei and micronucleated cells can, although delayed in cell cycle, sustain for multiple divisions.

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