Multiple origins of spontaneously arising micronuclei in HeLa cells: Direct evidence from long-term live cell imaging

2008 ◽  
Vol 646 (1-2) ◽  
pp. 41-49 ◽  
Author(s):  
Xiaotang Rao ◽  
Yingyin Zhang ◽  
Qiyi Yi ◽  
Heli Hou ◽  
Bo Xu ◽  
...  
Keyword(s):  
Hela Cells ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Direct Evidence ◽  
Live Cell ◽  
Multiple Origins

pH-triggered decomposition of polymeric fluorescent vesicles to induce growth of tetraphenylethylene nanoparticles for long-term live cell imaging

Polymer ◽  
2017 ◽  
Vol 118 ◽  
pp. 75-84 ◽  
Author(s):  
Xing Wang ◽  
Yanyu Yang ◽  
Fei Yang ◽  
Hong Shen ◽  
Decheng Wu
Keyword(s):  
Live Cell Imaging ◽  
Cell Imaging ◽  
Live Cell

Harvesting red fluorescence through design specific tuning of ICT and ESIPT: an efficient optical detection of cysteine and live cell imaging

RSC Advances ◽  
2016 ◽  
Vol 6 (98) ◽  
pp. 95722-95728 ◽  
Author(s):  
Uzra Diwan ◽  
Virendra Kumar ◽  
Rakesh K. Mishra ◽  
Nishant Kumar Rana ◽  
Biplob Koch ◽  
...  
Keyword(s):  
Fluorescent Probe ◽  
Hela Cells ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Optical Sensing ◽  
Optical Detection ◽  
Live Cell ◽  
Red Fluorescence ◽  
Bio Imaging ◽  
Ratiometric Fluorescent Probe

In this work, a 2-(2-hydroxyphenyl)benzothiazole (HBT)-based ratiometric fluorescent probe exhibiting coupling between the ICT and ESIPT mechanisms was exploited for the optical sensing of cysteine and successfully utilised in the bio-imaging of cysteine in HeLa cells.

scholarly journals Long-term fate of etoposide-induced micronuclei and micronucleated cells in Hela-H2B-GFP cells

2020 ◽  
Vol 94 (10) ◽  
pp. 3553-3561
Author(s):  
Hauke Reimann ◽  
Helga Stopper ◽  
Henning Hintzsche
Keyword(s):  
Cell Cycle ◽  
Cell Death ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Live Cell ◽  
Cellular Structures ◽  
Death Rates ◽  
Daughter Cells

Abstract Micronuclei are small nuclear cellular structures containing whole chromosomes or chromosomal fragments. While there is a lot of information available about the origin and formation of micronuclei, less is known about the fate of micronuclei and micronucleated cells. Possible fates include extrusion, degradation, reincorporation and persistence. Live cell imaging was performed to quantitatively analyse the fates of micronuclei and micronucleated cells occurring in vitro. Imaging was conducted for up to 96 h in HeLa-H2B-GFP cells treated with 0.5, 1 and 2 µg/ml etoposide. While a minority of micronuclei was reincorporated into the main nucleus during mitosis, the majority of micronuclei persisted without any alterations. Degradation and extrusion were observed rarely or never. The presence of micronuclei affected the proliferation of the daughter cells and also had an influence on cell death rates. Mitotic errors were found to be clearly increased in micronucleus-containing cells. The results show that micronuclei and micronucleated cells can, although delayed in cell cycle, sustain for multiple divisions.

scholarly journals Coplanarity driven fluorescence turn-on sensor for chromium(iii) and its application for bio-imaging

2018 ◽  
Vol 17 (2) ◽  
pp. 239-244 ◽  
Author(s):  
G. Balamurugan ◽  
S. Velmathi
Keyword(s):  
Hela Cells ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Fluorescence Enhancement ◽  
Live Cell ◽  
Turn On ◽  
Bio Imaging ◽  
Heteroaromatic Aldehyde ◽  
Fluorescence Turn On

The sensing behaviour of benzimidazole and thiozole derivatives from heteroaromatic aldehyde was studied towards various cations and anions. S1 showed selectivity towards Cr3+ with fluorescence enhancement and live cell imaging of Hela cells was successfully demonstrated.

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