Efficient synthesis of a long carbohydrate chain alkyl glycoside catalyzed by cyclodextrin glycosyltransferase (CGTase)

2009 ◽  
Vol 104 (5) ◽  
pp. 854-861 ◽  
Author(s):  
David Svensson ◽  
Stefan Ulvenlund ◽  
Patrick Adlercreutz
Keyword(s):  
Carbohydrate Chain ◽  
Efficient Synthesis ◽  
Cyclodextrin Glycosyltransferase ◽  
Alkyl Glycoside

scholarly journals Carbohydrate-Binding Module–Cyclodextrin Glycosyltransferase Fusion Enables Efficient Synthesis of 2-O-d-Glucopyranosyl-l-Ascorbic Acid with Soluble Starch as the Glycosyl Donor

2013 ◽  
Vol 79 (10) ◽  
pp. 3234-3240 ◽  
Author(s):  
Ruizhi Han ◽  
Jianghua Li ◽  
Hyun-Dong Shin ◽  
Rachel R. Chen ◽  
Guocheng Du ◽  
...  
Keyword(s):  
Ascorbic Acid ◽  
Soluble Starch ◽  
Carbohydrate Binding ◽  
Carbohydrate Binding Module ◽  
Efficient Synthesis ◽  
Wild Type ◽  
Cyclodextrin Glycosyltransferase ◽  
Content Type ◽  
Fusion Enzymes ◽  
Glycosyl Donor

ABSTRACTIn this study, we achieved the efficient synthesis of 2-O-d-glucopyranosyl-l-ascorbic acid (AA-2G) from soluble starch by fusing a carbohydrate-binding module (CBM) fromAlkalimonas amylolyticaα-amylase (CBMAmy) to cyclodextrin glycosyltransferase (CGTase) fromPaenibacillus macerans. One fusion enzyme, CGT-CBMAmy, was constructed by fusing the CBMAmyto the C-terminal region of CGTase, and the other fusion enzyme, CGTΔE-CBMAmy, was obtained by replacing the E domain of CGTase with CBMAmy. The two fusion enzymes were then used to synthesize AA-2G from soluble starch as a cheap and easily soluble glycosyl donor. Under the optimal conditions, the AA-2G yields produced using CGTΔE-CBMAmyand CGT-CBMAmywere 2.01 g/liter and 3.03 g/liter, respectively, which were 3.94- and 5.94-fold of the yield from the wild-type CGTase (0.51 g/liter). The reaction kinetics of the two fusion enzymes were analyzed and modeled to confirm the enhanced specificity toward soluble starch. It was also found that, compared to the wild-type CGTase, the two fusion enzymes had relatively high hydrolysis and disproportionation activities, factors that favor AA-2G synthesis. Finally, it was speculated that the enhancement of soluble starch specificity may be related to the changes of substrate binding ability and the substrate binding sites between the CBM and the starch granule.

A new facile and efficient synthesis of trans-(+)-sobrerol by biotransformation of α-pinene using extremophiles microorganisms

Planta Medica ◽  
2009 ◽  
Vol 75 (09) ◽  
Author(s):  
LA Vilaseca ◽  
J Quillaguamán ◽  
L Fuentes ◽  
O Sterner
Keyword(s):  
Efficient Synthesis

Highly efficient synthesis of methyl ethyl ketone oxime through ammoximation over Ti-MOR catalyst

2014 ◽  
Vol 34 (1) ◽  
pp. 243-250
Author(s):  
Jianghong DING ◽  
Le XU ◽  
Hao XU ◽  
Haihong WU ◽  
Yueming LIU ◽  
...  
Keyword(s):  
Methyl Ethyl Ketone ◽  
Methyl Ethyl ◽  
Efficient Synthesis ◽  
Highly Efficient

Advanced Heterocyclic Branched Semiconducting Units - Highly Efficient Synthesis and Physicochemical Characteristic

2013 ◽  
Vol 17 (3) ◽  
pp. 283-295 ◽  
Author(s):  
Jadwiga Soloducho ◽  
Joanna Cabaj ◽  
Kamila Olech ◽  
Przemyslaw Data ◽  
Mieczyslaw Lapkowski
Keyword(s):  
Physicochemical Characteristic ◽  
Efficient Synthesis ◽  
Highly Efficient

Facile and Efficient Synthesis of New Class of Imidazole Derivatives via One-Pot Multicomponent Reactions in Water

2013 ◽  
Vol 16 (10) ◽  
pp. 788-790
Author(s):  
Zinatossadat Hossaini ◽  
Samereh Seyfi ◽  
Faramarz Rostami-Charati ◽  
Mehdi Ghambarian
Keyword(s):  
Multicomponent Reactions ◽  
Efficient Synthesis ◽  
One Pot ◽  
Imidazole Derivatives ◽  
New Class
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