In silico multi-scale model of transport and dynamic seeding in a bone tissue engineering perfusion bioreactor

2012 ◽  
Vol 110 (4) ◽  
pp. 1221-1230 ◽  
Author(s):  
T.J. Spencer ◽  
L.A. Hidalgo-Bastida ◽  
S.H. Cartmell ◽  
I. Halliday ◽  
C.M. Care
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
In Silico ◽  
Scale Model ◽  
Perfusion Bioreactor ◽  
Multi Scale

Development of a Perfusion Bioreactor System for Alveolar Bone Tissue Engineering

2009 ◽  
Author(s):  
Ki Taek Lim ◽  
Pill Hoon Choung ◽  
Jang Ho Kim ◽  
Hyun Mok Son ◽  
Hoon Seonwoo ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Alveolar Bone ◽  
Perfusion Bioreactor ◽  
Bioreactor System

scholarly journals Continuum Modeling and Simulation in Bone Tissue Engineering

2019 ◽  
Vol 9 (18) ◽  
pp. 3674 ◽  
Author(s):  
Jose A. Sanz-Herrera ◽  
Esther Reina-Romo
Keyword(s):  
Tissue Engineering ◽  
Modeling And Simulation ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
In Silico ◽  
Assessment Tool ◽  
Numerical Models ◽  
Research Perspective

Bone tissue engineering is currently a mature methodology from a research perspective. Moreover, modeling and simulation of involved processes and phenomena in BTE have been proved in a number of papers to be an excellent assessment tool in the stages of design and proof of concept through in-vivo or in-vitro experimentation. In this paper, a review of the most relevant contributions in modeling and simulation, in silico, in BTE applications is conducted. The most popular in silico simulations in BTE are classified into: (i) Mechanics modeling and scaffold design, (ii) transport and flow modeling, and (iii) modeling of physical phenomena. The paper is restricted to the review of the numerical implementation and simulation of continuum theories applied to different processes in BTE, such that molecular dynamics or discrete approaches are out of the scope of the paper. Two main conclusions are drawn at the end of the paper: First, the great potential and advantages that in silico simulation offers in BTE, and second, the need for interdisciplinary collaboration to further validate numerical models developed in BTE.

Phase-induced porous composite microspheres sintered scaffold with protein–mineral interface for bone tissue engineering

RSC Advances ◽  
2015 ◽  
Vol 5 (28) ◽  
pp. 22005-22014 ◽  
Author(s):  
Janani Radhakrishnan ◽  
Gnana Santi Phani Deepika Gandham ◽  
Swaminathan Sethuraman ◽  
Anuradha Subramanian
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Porous Composite ◽  
Multi Scale ◽  
Composite Microspheres ◽  
Biomimetic Scaffolds

Phase induced porous composite microspheres were solvent/non-solvent sintered to construct 3D multi-scale porous biomimetic scaffolds with and without protein for bone tissue engineering.

Computational modeling of media flow through perfusion-based bioreactors for bone tissue engineering

2020 ◽  
Vol 234 (12) ◽  
pp. 1397-1408
Author(s):  
Hanieh Nokhbatolfoghahaei ◽  
Mahboubeh Bohlouli ◽  
Kazem Adavi ◽  
Zahrasadat Paknejad ◽  
Maryam Rezai Rad ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Shear Stress ◽  
Fluid Flow ◽  
Computational Modeling ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Fluid Motion ◽  
Shear Loading ◽  
Perfusion Bioreactor ◽  
Perfusion Bioreactors

Bioreactor system has been used in bone tissue engineering in order to simulate dynamic nature of bone tissue environments. Perfusion bioreactors have been reported as the most efficient types of shear-loading bioreactor. Also, combination of forces, such as rotation plus perfusion, has been reported to enhance cell growth and osteogenic differentiation. Mathematical modeling using sophisticated infrastructure processes could be helpful and streamline the development of functional grafts by estimating and defining an effective range of bioreactor settings for better augmentation of tissue engineering. This study is aimed to conduct computational modeling for newly designed bioreactors in order to alleviate the time and material consuming for evaluating bioreactor parameters and effect of fluid flow hydrodynamics (various amounts of shear stress) on osteogenesis. Also, biological assessments were performed in order to validate similar parameters under implementing the perfusion or rotating and perfusion fluid motions in bioreactors’ prototype. Finite element method was used to investigate the effect of hydrodynamic of fluid flow inside the bioreactors. The equations used in the simulation to calculate the velocity values and consequently the shear stress values include Navier–Stokes and Brinkman equations. It has been shown that rotational fluid motion in rotating and perfusion bioreactor produces more velocity and shear stress compared with perfusion bioreactor. Moreover, implementing the perfusion together with rotational force in rotating and perfusion bioreactors has been shown to have more cell proliferation and higher activity of alkaline phosphatase enzyme as well as formation of extra cellular matrix sheet, as an indicator of bone-like tissue formation.

Design and Assessment of a Dynamic Perfusion Bioreactor for Large Bone Tissue Engineering Scaffolds

2017 ◽  
Vol 185 (2) ◽  
pp. 555-563 ◽  
Author(s):  
Birru Bhaskar ◽  
Robert Owen ◽  
Hossein Bahmaee ◽  
Parcha Sreenivasa Rao ◽  
Gwendolen C. Reilly
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Perfusion Bioreactor ◽  
Tissue Engineering Scaffolds ◽  
Large Bone

HYDROXYAPATITE, ALGINATE, AND CHITOSAN FOR BONE SCAFFOLDS: SPECTROSCOPY STUDY

2016 ◽  
Vol 19 (2) ◽  
pp. 93-100
Author(s):  
Lalita El Milla
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Functional Groups ◽  
Bone Growth ◽  
Three Dimensional ◽  
Dimensional Structure ◽  
Tissue Engineering Scaffolds ◽  
Hydroxyapatite Powder ◽  
Materials Used

Scaffolds is three dimensional structure that serves as a framework for bone growth. Natural materials are often used in synthesis of bone tissue engineering scaffolds with respect to compliance with the content of the human body. Among the materials used to make scafffold was hydroxyapatite, alginate and chitosan. Hydroxyapatite powder obtained by mixing phosphoric acid and calcium hydroxide, alginate powders extracted from brown algae and chitosan powder acetylated from crab. The purpose of this study was to examine the functional groups of hydroxyapatite, alginate and chitosan. The method used in this study was laboratory experimental using Fourier Transform Infrared (FTIR) spectroscopy for hydroxyapatite, alginate and chitosan powders. The results indicated the presence of functional groups PO43-, O-H and CO32- in hydroxyapatite. In alginate there were O-H, C=O, COOH and C-O-C functional groups, whereas in chitosan there were O-H, N-H, C=O, C-N, and C-O-C. It was concluded that the third material containing functional groups as found in humans that correspond to the scaffolds material in bone tissue engineering.

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