Implantable hollow fiber bioreactor as a potential treatment for hypercholesterolemia: Characterization of the catalytic unit

1995 ◽  
Vol 48 (1) ◽  
pp. 36-41 ◽  
Author(s):  
Samuel D. Shefer ◽  
Vered Rosenberger ◽  
Gizette Vahanian ◽  
Wai T. Wong ◽  
Robert Langer
Keyword(s):  
Hollow Fiber ◽  
Potential Treatment ◽  
Hollow Fiber Bioreactor ◽  
Catalytic Unit

In-depth physiological characterization of primary human hepatocytes in a 3D hollow-fiber bioreactor

2011 ◽  
Vol 5 (8) ◽  
pp. e207-e218 ◽  
Author(s):  
Daniel Mueller ◽  
Georg Tascher ◽  
Ursula Müller-Vieira ◽  
Daniel Knobeloch ◽  
Andreas K. Nuessler ◽  
...  
Keyword(s):  
Hollow Fiber ◽  
Human Hepatocytes ◽  
Physiological Characterization ◽  
Hollow Fiber Bioreactor ◽  
Primary Human Hepatocytes

scholarly journals Homogenous overexpression of the extracellular matrix protein Netrin-1 in a hollow fiber bioreactor

2021 ◽  
Author(s):  
Aniel Moya-Torres ◽  
Monika Gupta ◽  
Fabian Heide ◽  
Natalie Krahn ◽  
Scott Legare ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Hollow Fiber ◽  
Mammalian Cells ◽  
Matrix Protein ◽  
Continuous Production ◽  
Extracellular Matrix Protein ◽  
Close Monitoring ◽  
High Quality ◽  
Biolayer Interferometry ◽  
Hollow Fiber Bioreactor

Abstract The production of recombinant proteins for functional and biophysical studies, especially in the field of structural determination, still represents a challenge as high quality and quantities are needed to adequately perform experiments. This is in part solved by optimizing protein constructs and expression conditions to maximize the yields in regular flask expression systems. Still, work flow and effort can be substantial with no guarantee to obtain improvements. This study presents a combination of workflows that can be used to dramatically increase protein production and improve processing results, specifically for the extracellular matrix protein Netrin-1. This proteoglycan is an axon guidance cue which interacts with various receptors to initiate downstream signaling cascades affecting cell differentiation, proliferation, metabolism, and survival. We were able to produce large glycoprotein quantities in mammalian cells, which were engineered for protein overexpression and secretion into the media using the controlled environment provided by a hollow fiber bioreactor. Close monitoring of the internal bioreactor conditions allowed for stable production over an extended period of time. In addition to this, Netrin-1 concentrations were monitored in expression media through biolayer interferometry which allowed us to increase Netrin-1 media concentrations tenfold over our current flask systems while preserving excellent protein quality and in solution behavior. Our particular combination of genetic engineering, cell culture system, protein purification, and biophysical characterization permitted us to establish an efficient and continuous production of high-quality protein suitable for structural biology studies that can be translated to various biological systems. Key points • Hollow fiber bioreactor produces substantial yields of homogenous Netrin-1 • Biolayer interferometry allows target protein quantitation in expression media • High production yields in the bioreactor do not impair Netrin-1 proteoglycan quality Graphical abstract

Preparation and characterization of nanoclay loaded polyacrylonitrile hollow fiber membranes

2021 ◽  
pp. 1-11
Author(s):  
Mahdi Nouri ◽  
Marjan Abbasi ◽  
Tara Aghebati ◽  
Mahsa Shirzadkhan
Keyword(s):  
Hollow Fiber ◽  
Hollow Fiber Membranes ◽  
Fiber Membranes

scholarly journals Design and characterization of ceramic hollow fiber membrane derived from waste ash using phase inversion-based extrusion/sintering technique for water filtration

2020 ◽  
pp. 1-18
Author(s):  
Zhong Sheng Tai ◽  
Mohd Hafiz Dzarfan Othman ◽  
Azeman Mustafa ◽  
Mohd Irfan Hatim Mohamed Dzahir ◽  
Siti Khadijah Hubadillah ◽  
...  
Keyword(s):  
Hollow Fiber ◽  
Phase Inversion ◽  
Hollow Fiber Membrane ◽  
Water Filtration ◽  
Fiber Membrane

Characterization of hydrophilic hollow fiber membranes prepared from poly(vinyl alcohol)

2010 ◽  
Vol 17 (1-3) ◽  
pp. 199-203 ◽  
Author(s):  
Mitsuru Higa ◽  
Kakuya Toyota ◽  
Takehiro Sugimoto
Keyword(s):  
Hollow Fiber ◽  
Vinyl Alcohol ◽  
Poly Vinyl Alcohol ◽  
Hollow Fiber Membranes ◽  
Fiber Membranes

Preparation and Characterization of Hydrophilic PVDF Hollow Fiber Ultrafiltration Membrane

2011 ◽  
Vol 480-481 ◽  
pp. 201-206
Author(s):  
Li Guo Wang ◽  
Xiu Ju Wang ◽  
Ai Min Wang ◽  
Wen Juan Liu ◽  
Shi Qi Guo ◽  
...  
Keyword(s):  
Acrylic Acid ◽  
Hollow Fiber ◽  
Polyvinylidene Fluoride ◽  
Breaking Strength ◽  
Pure Water ◽  
Water Flux ◽  
Technical Parameters ◽  
Rupture Pressure ◽  
Pure Water Flux

Hydrophilic Polyvinylidene fluoride (PVDF) hollow fiber ultrafiltration membranes were prepared by wet-spinning method. The effects of technical parameters of acrylic acid grafted onto PVDF on the performance of hydrophilic PVDF membranes were investigated via orthogonal test, the technical parameters of preparation of hydrophilic PVDF membranes were determined, and hydrophilic PVDF membranes were prepared. Then hydrophilic PVDF membranes were characterized in terms of breaking strength, breaking elongation, rupture pressure, pure water flux and rejection. The fouling properties and the conditions of acrylic acid grafted onto PVDF were also examined. The results showed that acrylic acid had been grafted onto PVDF, the breaking strength and rupture pressure improved greatly, and the fouling properties were better than PS hollow fiber UF membrane.

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