Combination of microwell structures and direct oxygenation enables efficient and size-regulated aggregate formation of an insulin-secreting pancreatic β-cell line

2013 ◽  
Vol 30 (1) ◽  
pp. 178-187 ◽  
Author(s):  
Marie Shinohara ◽  
Hiroshi Kimura ◽  
Kevin Montagne ◽  
Kikuo Komori ◽  
Teruo Fujii ◽  
...  
Keyword(s):  
Cell Line ◽  
Pancreatic Β Cell ◽  
Aggregate Formation ◽  
Β Cell

Effects of lipotoxicity on a novel insulin-secreting human pancreatic β-cell line, 1.1B4

2013 ◽  
Vol 394 (7) ◽  
pp. 909-918 ◽  
Author(s):  
Srividya Vasu ◽  
Neville H. McClenaghan ◽  
Jane T. McCluskey ◽  
Peter R. Flatt
Keyword(s):  
Mrna Expression ◽  
Cell Line ◽  
Molecular Mechanisms ◽  
Endoplasmic Reticulum Stress Response ◽  
Future Research ◽  
The Novel ◽  
Pancreatic Β Cell ◽  
Β Cell ◽  
Expression Of Genes ◽  
Cellular Ca

Abstract The novel insulin-secreting human pancreatic β-cell line, 1.1B4, demonstrates stability in culture and many of the secretory functional attributes of human pancreatic β-cells. This study investigated the cellular responses of 1.1B4 cells to lipotoxicity. Chronic 18-h exposure of 1.1B4 cells to 0.5 mm palmitate resulted in decreased cell viability and insulin content. Secretory responses to classical insulinotropic agents and cellular Ca2+ handling were also impaired. Palmitate decreased glucokinase activity and mRNA expression of genes involved in secretory function but up-regulated mRNA expression of HSPA5, EIF2A, and EIF2AK3, implicating activation of the endoplasmic reticulum stress response. Palmitate also induced DNA damage and apoptosis of 1.1B4 cells. These responses were accompanied by increased gene expression of the antioxidant enzymes SOD1, SOD2, CAT and GPX1. This study details molecular mechanisms underlying lipotoxicity in 1.1B4 cells and indicates the potential value of the novel β-cell line for future research.

Volume-sensitive amino acid efflux from a pancreatic β-cell line

2000 ◽  
Vol 162 (1-2) ◽  
pp. 201-208 ◽  
Author(s):  
A.C.G. Grant ◽  
J. Thomson ◽  
V.A. Zammit ◽  
D.B. Shennan
Keyword(s):  
Amino Acid ◽  
Cell Line ◽  
Pancreatic Β Cell ◽  
Β Cell ◽  
Amino Acid Efflux

scholarly journals Rapid Activation and Nuclear Translocation of Mitogen-activated Protein Kinases in Response to Physiological Concentration of Glucose in the MIN6 Pancreatic β Cell Line

1998 ◽  
Vol 273 (25) ◽  
pp. 15507-15513 ◽  
Author(s):  
Cyril Benes ◽  
Marie Paule Roisin ◽  
Huynh Van Tan ◽  
Claudine Creuzet ◽  
Jun-ichi Miyazaki ◽  
...  
Keyword(s):  
Cell Line ◽  
Protein Kinases ◽  
Nuclear Translocation ◽  
Pancreatic Β Cell ◽  
Β Cell ◽  
Physiological Concentration ◽  
Mitogen Activated Protein Kinases ◽  
Mitogen Activated Protein ◽  
Rapid Activation

scholarly journals Simultaneous analysis of membrane potential and calcium mobilization in pancreatic β-cell line MIN6.

1996 ◽  
Vol 71 ◽  
pp. 158
Author(s):  
Yasufimi Fukano ◽  
Tsuyoshi Azuma ◽  
Koichiro Ozawa ◽  
Jun-ichi Miyazaki ◽  
Tuntomu Masujima
Keyword(s):  
Membrane Potential ◽  
Cell Line ◽  
Calcium Mobilization ◽  
Simultaneous Analysis ◽  
Pancreatic Β Cell ◽  
Β Cell

scholarly journals Inhibition ol phosphatidylinositol 3-kinase by wonmannin enhances insulin secretion from the pancreatic β cell line MIN6

1995 ◽  
Vol 67 ◽  
pp. 18
Author(s):  
Takasin Sakurai ◽  
Shinji Hagiwara ◽  
Jun-ichi Miyazaki ◽  
Ynshiaki Nonomura
Keyword(s):  
Insulin Secretion ◽  
Cell Line ◽  
Pancreatic Β Cell ◽  
Phosphatidylinositol 3 Kinase ◽  
Β Cell ◽  
Phosphatidylinositol 3

Selective activation of Ca2+ influx by extracellular ATP in a pancreatic β-cell line (HIT)

1989 ◽  
Vol 1012 (1) ◽  
pp. 107-115 ◽  
Author(s):  
Jean-François Geschwind ◽  
Marcia Hiriart ◽  
Major C. Glennon ◽  
Habiba Najafi ◽  
Barbara E. Corkey ◽  
...  
Keyword(s):  
Cell Line ◽  
Extracellular Atp ◽  
Pancreatic Β Cell ◽  
Β Cell ◽  
Selective Activation

scholarly journals Cellular responses of novel human pancreatic β-cell line, 1.1B4 to hyperglycemia

Islets ◽  
2013 ◽  
Vol 5 (4) ◽  
pp. 170-177 ◽  
Author(s):  
Srividya Vasu ◽  
Neville H McClenaghan ◽  
Jane T McCluskey ◽  
Peter R Flatt
Keyword(s):  
Cell Line ◽  
Cellular Responses ◽  
Pancreatic Β Cell ◽  
Β Cell

scholarly journals Investigation of Ultrasound-Mediated Intracellular Ca2+ Oscillations in HIT-T15 Pancreatic β-Cell Line

Cells ◽  
2020 ◽  
Vol 9 (5) ◽  
pp. 1129
Author(s):  
Chi Woo Yoon ◽  
Nan Sook Lee ◽  
Kweon Mo Koo ◽  
Sunho Moon ◽  
Kyosuk Goo ◽  
...  
Keyword(s):  
Insulin Secretion ◽  
Cell Line ◽  
Purinergic Signaling ◽  
Underlying Mechanism ◽  
Resting Cells ◽  
Pancreatic Β Cell ◽  
Pulsed Ultrasound ◽  
Β Cell ◽  
Secretion Pathways ◽  
Glucose Stimulated Insulin Secretion

In glucose-stimulated insulin secretion (GSIS) of pancreatic β-cells, the rise of free cytosolic Ca2+ concentration through voltage-gated calcium channels (VGCCs) triggers the exocytosis of insulin-containing granules. Recently, mechanically induced insulin secretion pathways were also reported, which utilize free cytosolic Ca2+ ions as a direct regulator of exocytosis. In this study, we aimed to investigate intracellular Ca2+ responses on the HIT-T15 pancreatic β-cell line upon low-intensity pulsed ultrasound (LIPUS) stimulation and found that ultrasound induces two distinct types of intracellular Ca2+ oscillation, fast-irregular and slow-periodic, from otherwise resting cells. Both Ca2+ patterns depend on the purinergic signaling activated by the rise of extracellular ATP or ADP concentration upon ultrasound stimulation, which facilitates the release through mechanosensitive hemichannels on the plasma membrane. Further study demonstrated that two subtypes of purinergic receptors, P2X and P2Y, are working in a competitive manner depending on the level of glucose in the cell media. The findings can serve as an essential groundwork providing an underlying mechanism for the development of a new therapeutic approach for diabetic conditions with further validation.

scholarly journals The biphasic effect of wortmannin on insulin secretion from pancreatic β cell line MIN6.

1994 ◽  
Vol 64 ◽  
pp. 112
Author(s):  
Takashi Sakurai ◽  
Shinji Hagiwara ◽  
Fumi Tashiro ◽  
Yoshiaki Hashimoto ◽  
Hiroshi Yano ◽  
...  
Keyword(s):  
Insulin Secretion ◽  
Cell Line ◽  
Pancreatic Β Cell ◽  
Β Cell ◽  
Biphasic Effect
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