Microbial lipid extraction from Lipomyces starkeyi
 using irreversible electroporation

In this paper, potato starch wastewater as culture medium was treated by the oleaginous yeast Lipomyces starkeyi to biosynthesize microbial lipid. The result indicated that carbon source types, carbon source concentration, nitrogen source types, nitrogen source concentration, inoculum size, and cultivation time all had a significant effect on cell growth and microbial lipid accumulation in batch cultures. A measure of 120 g/L of glucose concentration, 3.0 g/L of (NH4)2SO4 concentration, 10% inoculum size, and incubation time 96 h cultivated in a shaking flask at 30 °C were found to be the optimal conditions not only for cell growth but also for lipid synthesis. Under this condition, the cellular biomass and lipid content could reach 2.59 g/L and 8.88%, respectively. This work provides a new method for effective utilization of potato starch wastewater, which has particular social and economic benefits for yeast treatment technology.

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Microbial treatment of the monosodium glutamate wastewater by Lipomyces starkeyi to produce microbial lipid

Bioresource Technology ◽

10.1016/j.biortech.2011.12.022 ◽

2012 ◽

Vol 106 ◽

pp. 69-73 ◽

Cited By ~ 22

Author(s):

Jun-Xian Liu ◽

Qin-Yan Yue ◽

Bao-Yu Gao ◽

Zuo-Hao Ma ◽

Pei-Dong Zhang

Keyword(s):

Monosodium Glutamate ◽

Microbial Lipid ◽

Lipomyces Starkeyi

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Lipid Extraction Methods for Lipomyces starkeyi

Agricultural and Biological Chemistry ◽

10.1271/bbb1961.37.2653 ◽

1973 ◽

Vol 37 (11) ◽

pp. 2653-2656 ◽

Cited By ~ 6

Author(s):

Tetsuya SUZUKI ◽

Akihiro TAKIGAWA ◽

Kiyozo HASEGAWA

Keyword(s):

Lipid Extraction ◽

Extraction Methods ◽

Lipomyces Starkeyi

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Microbial lipid production by the yeast Lipomyces starkeyi InaCC Y604 grown on various carbon sources

IOP Conference Series Earth and Environmental Science ◽

10.1088/1755-1315/762/1/012073 ◽

2021 ◽

Vol 762 (1) ◽

pp. 012073

Author(s):

A B Juanssilfero ◽

P Salsabila ◽

E Agustriana ◽

A Andriani ◽

Fahrurrozi ◽

...

Keyword(s):

Lipid Production ◽

Carbon Sources ◽

Microbial Lipid ◽

Lipomyces Starkeyi ◽

Microbial Lipid Production

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Formulation of fermentation media from flour-rich waste streams for microbial lipid production by Lipomyces starkeyi

Journal of Biotechnology ◽

10.1016/j.jbiotec.2014.08.011 ◽

2014 ◽

Vol 189 ◽

pp. 36-45 ◽

Cited By ~ 63

Author(s):

Sofia Tsakona ◽

Nikolaos Kopsahelis ◽

Afroditi Chatzifragkou ◽

Seraphim Papanikolaou ◽

Ioannis K. Kookos ◽

...

Keyword(s):

Lipid Production ◽

Microbial Lipid ◽

Lipomyces Starkeyi ◽

Waste Streams ◽

Microbial Lipid Production ◽

Fermentation Media

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A Resorcinol-Formaldehyde Resin as an Embedding Material for Electron Microscopy of Membranes

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s042482010006355x ◽

1969 ◽

Vol 27 ◽

pp. 328-329 ◽

Cited By ~ 1

Author(s):

J. G. Robertson ◽

D. F. Parsons

Keyword(s):

Electron Microscopy ◽

Osmium Tetroxide ◽

Neutral Lipids ◽

Lipid Extraction ◽

Water Soluble ◽

Formaldehyde Resin ◽

Electron Microscope Autoradiography ◽

Resorcinol Formaldehyde ◽

Major Disadvantage ◽

Cell Organization

The extraction of lipids from tissues during fixation and embedding for electron microscopy is widely recognized as a source of possible artifact, especially at the membrane level of cell organization. Lipid extraction is also a major disadvantage in electron microscope autoradiography of radioactive lipids, as in studies of the uptake of radioactive fatty acids by intestinal slices. Retention of lipids by fixation with osmium tetroxide is generally limited to glycolipids, phospholipids and highly unsaturated neutral lipids. Saturated neutral lipids and sterols tend to be easily extracted by organic dehydrating reagents prior to embedding. Retention of the more saturated lipids in embedded tissue might be achieved by developing new cross-linking reagents, by the use of highly water soluble embedding materials or by working at very low temperatures.

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