scholarly journals Lipid Extraction Methods for Lipomyces starkeyi

1973 ◽  
Vol 37 (11) ◽  
pp. 2653-2656 ◽  
Author(s):  
Tetsuya SUZUKI ◽  
Akihiro TAKIGAWA ◽  
Kiyozo HASEGAWA
Keyword(s):  
Lipid Extraction ◽  
Extraction Methods ◽  
Lipomyces Starkeyi

scholarly journals Physical Methods of Microalgal Biomass Pretreatment

2014 ◽  
Vol 28 (3) ◽  
pp. 341-348 ◽  
Author(s):  
Agata Piasecka ◽  
Izabela Krzemińska ◽  
Jerzy Tys
Keyword(s):  
Alternative Energy ◽  
Lipid Extraction ◽  
Physical Method ◽  
Extraction Methods ◽  
Biodiesel Production ◽  
Biomass Pretreatment ◽  
Microalgal Biomass ◽  
Alternative Energy Sources ◽  
Wet Biomass ◽  
Microwave Techniques

Abstract The prospect of depletion of natural energy resources on the Earth forces researchers to seek and explore new and alternative energy sources. Biomass is a composite resource that can be used in many ways leading to diversity of products. Therefore, microalgal biomass offers great potential. The main aim of this study is to find the best physical method of microalgal biomass pretreatment that guarantees efficient lipid extraction. These studies identifies biochemical composition of microalgal biomass as source for biodisel production. The influence of drying at different temperatures and lyophilization was investigated. In addition, wet and untreated biomass was examined. Cell disruption (sonication and microwave) techniques were used to improve lipid extraction from wet biomass. Additionally, two different extraction methods were carried out to select the best method of crude oil extraction. The results of this study show that wet biomass after sonication is the most suitable for extraction. The fatty acid composition of microalgal biomass includes linoleic acid (C18:2), palmitic acid (C16:0), oleic acid (C18:1), linolenic acid (C18:3), and stearic acid (C18:0), which play a key role in biodiesel production.

scholarly journals Comparing Extraction Methods for Biomarker Steroid Characterisation from Soil and Slurry

2020 ◽  
Vol 231 (10) ◽  
Author(s):  
Amber Manley ◽  
Adrian L. Collins ◽  
Adrian Joynes ◽  
Per-Erik Mellander ◽  
Phil Jordan
Keyword(s):  
Water Quality ◽  
Lipid Extraction ◽  
Cost Savings ◽  
Soxhlet Extraction ◽  
Extraction Methods ◽  
Significant Difference ◽  
Two Factors ◽  
Precious Resource ◽  
Research Gap ◽  
Block Experiment

Abstract Clean water is a precious resource, and policies/programmes are implemented worldwide to protect and/or improve water quality. Faecal pollution can be a key contributor to water quality decline causing eutrophication through nutrient enrichment and pathogenic contamination. The robust sourcing of faecal pollutants is important to be able to target the appropriate sector and to engage managers. Biomarker technology has the potential for source confirmation, by using, for example the biomarker suite of steroids. Steroids have been used in the differentiation of human and animal faeces; however, there is no unequivocal extraction technique. Some of the methods used include (i) Soxhlet extraction, (ii) Bligh and Dyer (BD) extraction, and (iii) accelerated solvent extraction (ASE). The less costly and time intensive technique of ASE is particularly attractive, but a current research gap concerns further comparisons regarding ASE lipid extraction from soils/slurries compared with the more traditional Soxhlet and BD extractions. Accordingly, a randomised complete block experiment was implemented to assess differences between the three extraction methods, differences between the different sample types, and the interactions between these two factors. Following GC-MS, it was found that there was no significant difference between the results of the steroid extraction methods, regardless of the type of sample used, for the quantity of each steroid extracted. It was concluded that ASE could be used confidently instead of the more established steroid extraction methods, thereby delivering time and cost savings.

scholarly journals Extraction of Lipids from Liquid Biological Samples for High-Throughput Lipidomics

Molecules ◽  
2020 ◽  
Vol 25 (14) ◽  
pp. 3192
Author(s):  
Samuel Furse ◽  
Adam J. Watkins ◽  
Albert Koulman
Keyword(s):  
High Throughput ◽  
Lipid Fraction ◽  
Lipid Extraction ◽  
Extraction Methods ◽  
Mouse Heart ◽  
Aqueous Fraction ◽  
Chlorinated Solvent ◽  
Aqueous Component ◽  
The Difference ◽  
Better Than

Extraction of the lipid fraction is a key part of acquiring lipidomics data. High-throughput lipidomics, the extraction of samples in 96w plates that are then run on 96 or 384w plates, has particular requirements that mean special development work is needed to fully optimise an extraction method. Several methods have been published as suitable for it. Here, we test those methods using four liquid matrices: milk, human serum, homogenised mouse liver and homogenised mouse heart. In order to determine the difference in performance of the methods as objectively as possible, we used the number of lipid variables identified, the total signal strength and the coefficient of variance to quantify the performance of the methods. This showed that extraction methods with an aqueous component were generally better than those without for these matrices. However, methods without an aqueous fraction in the extraction were efficient for milk samples. Furthermore, a mixture containing a chlorinated solvent (dichloromethane) appears to be better than an ethereal solvent (tert-butyl methyl ether) for extracting lipids. This study suggests that a 3:1:0.005 mixture of dichloromethane, methanol and triethylammonium chloride, with an aqueous wash, is the most efficient of the currently reported methods for high-throughput lipid extraction and analysis. Further work is required to develop non-aqueous extraction methods that are both convenient and applicable to a broad range of sample types.

scholarly journals Comparison of Conventional and Sustainable Lipid Extraction Methods for the Production of Oil and Protein Isolate from Edible Insect Meal

Foods ◽  
2019 ◽  
Vol 8 (11) ◽  
pp. 572 ◽  
Author(s):  
Laroche ◽  
Perreault ◽  
Marciniak ◽  
Gravel ◽  
Chamberland ◽  
...  
Keyword(s):  
Supercritical Co2 ◽  
Protein Extraction ◽  
Lipid Extraction ◽  
Tenebrio Molitor ◽  
Extraction Methods ◽  
Extraction Yield ◽  
Acheta Domesticus ◽  
Human Consumption ◽  
Alternative Source ◽  
Edible Insect

Edible insects represent an interesting alternative source of protein for human consumption but the main hurdle facing the edible insect sector is low consumer acceptance. However, increased acceptance is anticipated when insects are incorporated as a processed ingredient, such as protein-rich powder, rather than presented whole. To produce edible insect fractions with high protein content, a defatting step is necessary. This study investigated the effects of six defatting methods (conventional solvents, three-phase partitioning, and supercritical CO2) on lipid extraction yield, fatty profiles, and protein extraction and purification of house cricket (Acheta domesticus) and mealworm (Tenebrio molitor) meals. Ethanol increased the lipid extraction yield (22.7%–28.8%), irrespective of the insect meal used or the extraction method applied. Supercritical CO2 gave similar lipid extraction yields as conventional methods for Tenebrio molitor (T. molitor) (22.1%) but was less efficient for Acheta domesticus (A. domesticus) (11.9%). The protein extraction yield ranged from 12.4% to 38.9% for A. domesticus, and from 11.9% to 39.3% for T. molitor, whereas purification rates ranged from 58.3% to 78.5% for A. domesticus and from 48.7% to 75.4% for T. molitor.

Effects of Different Biomass Drying and Lipid Extraction Methods on Algal Lipid Yield, Fatty Acid Profile, and Biodiesel Quality

2015 ◽  
Vol 175 (6) ◽  
pp. 3048-3057 ◽  
Author(s):  
Javid Hussain ◽  
Yan Liu ◽  
Wilson A. Lopes ◽  
Janice I. Druzian ◽  
Carolina O. Souza ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Fatty Acid Profile ◽  
Lipid Extraction ◽  
Extraction Methods ◽  
Lipid Yield ◽  
Algal Lipid ◽  
Biodiesel Quality

scholarly journals A comparative study: the impact of different lipid extraction methods on current microalgal lipid research

2014 ◽  
Vol 13 (1) ◽  
pp. 14 ◽  
Author(s):  
Yan Li ◽  
Forough Ghasemi Naghdi ◽  
Sourabh Garg ◽  
Tania Adarme-Vega ◽  
Kristofer J Thurecht ◽  
...  
Keyword(s):  
Comparative Study ◽  
Lipid Extraction ◽  
Extraction Methods ◽  
Lipid Research ◽  
The Impact

Comparison of Different Procedures for the Lipid Extraction from HL-60 Cells: A MALDI-TOF Mass Spectrometric Study

2005 ◽  
Vol 60 (1-2) ◽  
pp. 143-152 ◽  
Author(s):  
Marijana Petković ◽  
Andreas Vocks ◽  
Matthias Müller ◽  
Jürgen Schiller ◽  
Jürgen Arnhold
Keyword(s):  
Cell Line ◽  
Lipid Extraction ◽  
Mass Spectrometric Study ◽  
Extraction Methods ◽  
Cellular Systems ◽  
Leukaemia Cell Line ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Chloroform Methanol ◽  
Tof Ms

A human leukaemia cell line - HL-60 - can be differentiated into neutrophils or macrophages and both differentiation processes are accompanied by changes of the lipid composition. Various methods were described for the extraction of lipids from cellular systems, but only two of them were applied to the HL-60 cell line so far. In this study we compared five selected extraction methods for the lipid extraction from HL-60 cells with regard to their qualitative analysis by matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry (MALDI-TOF MS): chloroform/methanol at volume ratios 2:1 and 1:2, isopropanol/ chloroform, isopropanol/hexane and butanol. In addition, the cholesterol and phospholipid concentrations in organic extracts were measured by colorimetric assays. Results can be summarized as follows: For the analysis of polar phospholipids obtained from HL-60 cells by MALDI-TOF MS, a chlorofom/methanol (1:2) or isopropanol/chloroform mixture or butanol can be applied as extraction systems. On the other hand, if one would like to analyze changes in triacylglycerols, then chloroform/methanol (2:1) would be the method of choice.

A comparative study of lipid extraction methods for the quantification of steroidal biomarkers within soil and cattle slurry

2020 ◽  
Author(s):  
Amber Manley ◽  
Adrian Collins ◽  
Adrian Joynes ◽  
Per-Erik Mellander ◽  
Phil Jordan
Keyword(s):  
Water Quality ◽  
Lipid Extraction ◽  
Cost Savings ◽  
Soxhlet Extraction ◽  
Extraction Methods ◽  
Significant Difference ◽  
Two Factors ◽  
Precious Resource ◽  
Research Gap ◽  
Block Experiment

<p>Clean water is a precious resource and policies/programmes are implemented worldwide to protect and/or improve water quality. Faecal pollution can be a key contributor to water quality decline causing eutrophication through nutrient enrichment and also pathogenic contamination. The robust sourcing of faecal pollutants is important to be able to target the appropriate sector and to engage managers.  Biomarker technology has the potential for source confirmation, by using, for example, the biomarker suite of steroids. Steroids have been used in the differentiation of human and animal faeces; however, there is no unequivocal extraction technique regarding either suite’s analysis. Some of the methods used include: i) Soxhlet extraction, ii) Bligh and Dyer (BD) extraction, and iii) accelerated solvent extraction (ASE). The less costly and time intensive technique of ASE is particularly attractive, but a current research gap concerns further comparisons regarding ASE lipid extraction of soils/slurries compared with the more traditionally used methods of Soxhlet and BD extraction. Accordingly, a randomised complete block experiment was implemented to assess for differences between the three extraction methods, differences between the different sample types, and the interactions between these two factors. Following GC-MS, it was found that there was no significant difference between the steroid extraction method used, regardless of the type of sample used, for the quantity of each steroid extracted. It was concluded that ASE could be used confidently instead of the more established steroid extraction methods, thereby delivering time and cost savings.</p>

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