Consolidated plasmid Design for Stabilized Heterologous Production of the complex natural product Siderophore Yersiniabactin

2020 ◽  
Author(s):  
Ruiquan Qi ◽  
Girish Swayambhu ◽  
Michael Bruno ◽  
Guojian Zhang ◽  
Blaine A. Pfeifer
Keyword(s):  
Natural Product ◽  
Heterologous Production ◽  
Plasmid Design

scholarly journals A chromatogram-simplified Streptomyces albus host for heterologous production of natural products

2019 ◽  
Author(s):  
Asif Fazal ◽  
Divya Thankachan ◽  
Ellie Harris ◽  
Ryan F. Seipke
Keyword(s):  
Natural Products ◽  
Natural Product ◽  
Gene Clusters ◽  
Heterologous Production ◽  
Biosynthetic Gene ◽  
Critical Aspect ◽  
Biosynthetic Gene Clusters ◽  
Streptomyces Albus ◽  
Microbial Natural Products ◽  
Suitable Expression

AbstractCloning natural product biosynthetic gene clusters from cultured or uncultured sources and their subsequent expression by genetically tractable heterologous hosts is an essential strategy for the elucidation and characterisation of novel microbial natural products. The availability of suitable expression hosts is a critical aspect of this workflow. In this work, we mutagenised five endogenous biosynthetic gene clusters from Streptomyces albus S4, which reduced the complexity of chemical extracts generated from the strain and eliminated antifungal and antibacterial bioactivity. We showed that the resulting quintuple mutant can express foreign BGCs by heterologously producing actinorhodin, cinnamycin and prunustatin. We envisage that our strain will be a useful addition to the growing suite of heterologous expression hosts available for exploring microbial secondary metabolism.

scholarly journals A chromatogram-simplified Streptomyces albus host for heterologous production of natural products

2019 ◽  
Vol 113 (4) ◽  
pp. 511-520 ◽  
Author(s):  
Asif Fazal ◽  
Divya Thankachan ◽  
Ellie Harris ◽  
Ryan F. Seipke
Keyword(s):  
Natural Products ◽  
Natural Product ◽  
Gene Clusters ◽  
Heterologous Production ◽  
Biosynthetic Gene ◽  
Critical Aspect ◽  
Biosynthetic Gene Clusters ◽  
Streptomyces Albus ◽  
Microbial Natural Products ◽  
Suitable Expression

AbstractCloning natural product biosynthetic gene clusters from cultured or uncultured sources and their subsequent expression by genetically tractable heterologous hosts is an essential strategy for the elucidation and characterisation of novel microbial natural products. The availability of suitable expression hosts is a critical aspect of this workflow. In this work, we mutagenised five endogenous biosynthetic gene clusters from Streptomyces albus S4, which reduced the complexity of chemical extracts generated from the strain and eliminated antifungal and antibacterial bioactivity. We showed that the resulting quintuple mutant can express foreign biosynthetic gene clusters by heterologously producing actinorhodin, cinnamycin and prunustatin. We envisage that our strain will be a useful addition to the growing suite of heterologous expression hosts available for exploring microbial secondary metabolism.

scholarly journals Heterologous Production of 4-O-Demethylbarbamide, a Marine Cyanobacterial Natural Product

2012 ◽  
Vol 14 (23) ◽  
pp. 5824-5827 ◽  
Author(s):  
Eun Ji Kim ◽  
Jong Hyun Lee ◽  
Hyukjae Choi ◽  
Alban R. Pereira ◽  
Yeon Hee Ban ◽  
...  
Keyword(s):  
Natural Product ◽  
Heterologous Production

Biosynthesis and heterologous production of furanocoumarins: perspectives and current challenges

2020 ◽  
Author(s):  
Joana L. Rodrigues ◽  
Lígia R. Rodrigues
Keyword(s):  
Heterologous Production

This highlight reviews the furanocoumarins pathway and explores the challenges to address toward their heterologous production.

Synthetic studies toward inducamide C

2021 ◽  
Author(s):  
Ardalan A. Nabi ◽  
Lydia M. Scott ◽  
Daniel P. Furkert ◽  
Jonathan Sperry
Keyword(s):  
Natural Product ◽  
Structural Revision ◽  
Synthetic Studies

The rare benzoxazepine ring in the alkaloid inducamide C is unstable and prone to rearrangement, indicating that structural revision of the natural product may be necessary.

Lessons learned from the optimization of natural product based kinase inhibitors through molecular calculations

Planta Medica ◽  
2008 ◽  
Vol 74 (09) ◽  
Author(s):  
V Myrianthopoulos ◽  
P Magiatis ◽  
AL Skaltsounis ◽  
L Meijer ◽  
E Mikros
Keyword(s):  
Natural Product ◽  
Kinase Inhibitors ◽  
Lessons Learned ◽  
Molecular Calculations

Pharmacoproteomic and toxicoproteomic study of the natural product Ebenfuran III in DU-145 prostate cancer cells using iTRAQ with 2D LC and tandem mass spectrometry

Planta Medica ◽  
2008 ◽  
Vol 74 (09) ◽  
Author(s):  
M Halabalaki ◽  
IT Roumeliotis ◽  
E Giannopoulou ◽  
X Alexi ◽  
L Meijer ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Mass Spectrometry ◽  
Tandem Mass Spectrometry ◽  
Cancer Cells ◽  
Natural Product ◽  
Tandem Mass ◽  
Prostate Cancer Cells

Protective effects of ligustilide, a natural product from Aaugellica sinensis (Oliv.) Diels, in a rabbit model of endotoxic shock

Planta Medica ◽  
2009 ◽  
Vol 75 (09) ◽  
Author(s):  
M Shao ◽  
JF Liu ◽  
HB Zhu ◽  
K Liu
Keyword(s):  
Natural Product ◽  
Endotoxic Shock ◽  
Rabbit Model ◽  
Protective Effects
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