Resveratrol and Celastrol Loaded Collagen Dental Implants Regulate Periodontal Ligament Fibroblast Growth and Osteoclastogenesis of Bone Marrow Macrophages

2020 ◽  
Author(s):  
Ruijie Wang ◽  
Bin Bao ◽  
Chunling Bao ◽  
Shujun Wang ◽  
Saeed Ur Rahman ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Dental Implants ◽  
Periodontal Ligament ◽  
Fibroblast Growth ◽  
Periodontal Ligament Fibroblast

scholarly journals Basic fibroblast growth factor may be an endogenous radioprotector of normal tissue during bone marrow transplant

1998 ◽  
Vol 42 (1) ◽  
pp. 195
Author(s):  
P. Okunieff ◽  
J.A. Barrett ◽  
S.E. Phang ◽  
A. Li ◽  
L.S. Constine ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Growth Factor ◽  
Fibroblast Growth Factor ◽  
Bone Marrow Transplant ◽  
Basic Fibroblast Growth Factor ◽  
Normal Tissue ◽  
Marrow Transplant ◽  
Fibroblast Growth

scholarly journals Osteoclastic effects of mBMMSCs under compressive pressure during orthodontic tooth movement

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Jing Wang ◽  
Delong Jiao ◽  
Xiaofeng Huang ◽  
Yuxing Bai
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Normal Saline ◽  
Periodontal Ligament ◽  
Alveolar Bone ◽  
Tooth Movement ◽  
Orthodontic Tooth Movement ◽  
Mouse Bone Marrow ◽  
Micro Ct ◽  
Tail Vein

Abstract Background During orthodontic tooth movement (OTM), alveolar bone remodelling is closely related to mechanical force. It is unclear whether stem cells can affect osteoclastogenesis to promote OTM. This study aimed to investigate the role of mouse bone marrow mesenchymal stem cells (mBMMSCs) under compression load in OTM. Methods A mouse OTM model was established, and GFP-labelled mBMMSCs and normal saline were injected into different groups of mice by tail vein injection. OTM distance was measured using tissue specimens and micro-computed tomography (micro-CT). The locations of mBMMSCs were traced using GFP immunohistochemistry. Haematoxylin-eosin staining, tartrate-resistant acid phosphate (TRAP) staining and immunohistochemistry of Runx2 and lipoprotein lipase were used to assess changes in the periodontal ligament during OTM. mBMMSCs under compression were co-cultured with mouse bone marrow-derived macrophages (mBMMs), and the gene expression levels of Rankl, Mmp-9, TRAP, Ctsk, Alp, Runx2, Ocn and Osterix were determined by RT-PCR. Results Ten days after mBMMSCs were injected into the tail vein of mice, the OTM distance increased from 176 (normal saline) to 298.4 μm, as determined by tissue specimen observation, and 174.2 to 302.6 μm, as determined by micro-CT metrological analysis. GFP-labelled mBMMSCs were mostly located on the compressed side of the periodontal ligament. Compared to the saline group, the number of osteoclasts in the alveolar bone increased significantly (P < 0.01) on the compressed side in the mBMMSC group. Three days after mBMMSC injection, the number of Runx2-GFP double-positive cells on the tension side was significantly higher than that on the compression side. After applying compressive force on the mBMMSCs in vitro for 2 days, RANKL expression was significantly higher than in the non-compression cells, but expression of Alp, Runx2, Ocn and Osterix was significantly decreased (P < 0.05). The numbers of osteoclasts differentiated in response to mBMMs co-cultured with mBMMSCs under pressure load and expression of osteoclast differentiation marker genes (Mmp-9, TRAP and Ctsk) were significantly higher than those in mBMMs stimulated by M-CSF alone (P < 0.05). Conclusions mBMMSCs are not only recruited to the compressed side of the periodontal ligament but can also promote osteoclastogenesis by expressing Rankl, improving the efficiency of OTM.

Differential Effect of Water-Soluble Chitin on Collagen Synthesis of Human Bone Marrow Stem Cells and Human Periodontal Ligament Stem Cells

2015 ◽  
Vol 21 (3-4) ◽  
pp. 451-462 ◽  
Author(s):  
So-Yon Park ◽  
Jung-Chul Park ◽  
Min-Soo Kim ◽  
Sung-Eun Lee ◽  
Ki-Joon Kim ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Periodontal Ligament ◽  
Differential Effect ◽  
Collagen Synthesis ◽  
Bone Marrow Stem Cells ◽  
Water Soluble ◽  
Human Bone ◽  
Human Bone Marrow ◽  
Periodontal Ligament Stem Cells

Functional Behaviour of Human Periodontal Ligament Cells around Various Dental Implants

2007 ◽  
Vol 361-363 ◽  
pp. 837-840 ◽  
Author(s):  
Xiao Ting Luo ◽  
Zhen Gao ◽  
Shi Gui Yan ◽  
Wei Deng ◽  
Wen Shu Zhang ◽  
...  
Keyword(s):  
Surface Roughness ◽  
Dental Implants ◽  
Periodontal Ligament ◽  
Cell Counting ◽  
Immunofluorescent Staining ◽  
Periodontal Ligament Cells ◽  
Human Periodontal Ligament Cells ◽  
Proliferation And Differentiation ◽  
In Vitro Systems

In the present investigation, four titanium (Ti) surfaces of dental implants were compared through in vitro systems. The surface roughness of Ti was measured by TR240 mobile surface roughmeter. The Ti implants were seeded with human periodontal ligament cells (hPLDCs) and maintained for a period of 0-7 days. The adhesion, proliferation, and differentiation of hPLDCs were observed by using Cell morphology, cell counting and Osteocalcin (OC) immunofluorescent staining. Results suggest that surface roughness of titanium favors hPDLCs behavior and improves cell adhesion, proliferation, and differentiation.

Effect of HBSS storage time on human periodontal ligament fibroblast viability

2010 ◽  
Vol 26 (6) ◽  
pp. 481-483 ◽  
Author(s):  
Beatriz Dulcineia Mendes De Souza ◽  
Eduardo Antunes Bortoluzzi ◽  
Cleonice Da Silveira Teixeira ◽  
Wilson Tadeu Felippe ◽  
Cláudia Maria Oliveira Simões ◽  
...  
Keyword(s):  
Periodontal Ligament ◽  
Storage Time ◽  
Human Periodontal Ligament Fibroblast ◽  
Periodontal Ligament Fibroblast
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