Nuclear delivery of plasmid DNA determines the efficiency of gene expression

2019 ◽  
Vol 43 (7) ◽  
pp. 789-798 ◽  
Author(s):  
Hui Liu ◽  
Zhen Yang ◽  
Zhe Xun ◽  
Zihao Gao ◽  
Yanping Sun ◽  
...  
Keyword(s):  
Gene Expression ◽  
Plasmid Dna

Linear forms of plasmid DNA are superior to supercoiled structures as active templates for gene expression in plant protoplasts

1988 ◽  
Vol 11 (4) ◽  
pp. 517-527 ◽  
Author(s):  
Nurit Ballas ◽  
Nehama Zakai ◽  
Devorah Friedberg ◽  
Abraham Loyter
Keyword(s):  
Gene Expression ◽  
Plasmid Dna ◽  
Plant Protoplasts ◽  
Linear Forms

High Levels of Foreign Gene Expression in Hepatocytes after Tail Vein Injections of Naked Plasmid DNA

1999 ◽  
Vol 10 (10) ◽  
pp. 1735-1737 ◽  
Author(s):  
Guofeng Zhang ◽  
Vladimir Budker ◽  
Jon A. Wolff
Keyword(s):  
Gene Expression ◽  
Plasmid Dna ◽  
Foreign Gene ◽  
Foreign Gene Expression ◽  
Tail Vein ◽  
Naked Plasmid ◽  
Naked Plasmid Dna

scholarly journals Successful transfer of plasmid DNA intoin vitrocells transfected with an inorganic plasmid–Mg/Al-LDH nanobiocomposite material as a vector for gene expression

2008 ◽  
Vol 20 (4) ◽  
pp. 045602 ◽  
Author(s):  
Mas Jaffri Masarudin ◽  
Khatijah Yusoff ◽  
Raha Abdul Rahim ◽  
Mohd Zobir Hussein
Keyword(s):  
Gene Expression ◽  
Plasmid Dna ◽  
Successful Transfer

Enhancements in Gene Expression by the Choice of Plasmid DNA Formulations Containing Neutral Polymeric Excipients

2002 ◽  
Vol 91 (5) ◽  
pp. 1371-1381 ◽  
Author(s):  
Chin-Yi Huang ◽  
San San Ma ◽  
Sandra Lee ◽  
Ramachandran Radhakrishnan ◽  
Chad S. Braun ◽  
...  
Keyword(s):  
Gene Expression ◽  
Plasmid Dna

Efficient and sustained gene expression in primary T lymphocytes and primary and cultured tumor cells mediated by adeno-associated virus plasmid DNA complexed to cationic liposomes

1994 ◽  
Vol 14 (4) ◽  
pp. 2411-2418
Author(s):  
R Philip ◽  
E Brunette ◽  
L Kilinski ◽  
D Murugesh ◽  
M A McNally ◽  
...  
Keyword(s):  
Gene Expression ◽  
Tumor Cells ◽  
Plasmid Dna ◽  
Interleukin 2 ◽  
Cationic Liposomes ◽  
Lymphoid Cells ◽  
Chromosomal Dna ◽  
Adeno Associated Virus ◽  
Cultured Tumor Cells

We have used cationic liposomes to facilitate adeno-associated virus (AAV) plasmid transfections of primary and cultured cell types. AAV plasmid DNA complexed with liposomes showed levels of expression several fold higher than those of complexes with standard plasmids. In addition, long-term expression (> 30 days) of the gene, unlike the transient expression demonstrated by typical liposome-mediated transfection with standard plasmids, was observed. Southern analysis of chromosomal DNA further substantiated the hypothesis that the long-term expression was due to the presence of the transgene in the AAV plasmid-transfected group and not in the standard plasmid-transfected group. AAV plasmid-liposome complexes induced levels of transgene expression comparable to those obtained by recombinant AAV transduction. Primary breast, ovarian, and lung tumor cells were transfectable with the AAV plasmid DNA-liposome complexes. Transfected primary and cultured tumor cells were able to express transgene product even after lethal irradiation. High-level gene expression was also observed in freshly isolated CD3+, CD4+, and CD8+ T cells from normal human peripheral blood. Transfection efficiency ranged from 10 to 50% as assessed by intracellular interleukin-2 levels in interleukin-2-transfected cells. The ability to express transgenes in primary tumor and lymphoid cells may be applied toward tumor vaccine studies and protocols which may eventually permit highly specific modulation of the cellular immune response in cancer and AIDS.

scholarly journals Gene Transfer into the Lung by Nanoparticle Dextran-Spermine/Plasmid DNA Complexes

2010 ◽  
Vol 2010 ◽  
pp. 1-10 ◽  
Author(s):  
Syahril Abdullah ◽  
Wai Yeng Wendy-Yeo ◽  
Hossein Hosseinkhani ◽  
Mohsen Hosseinkhani ◽  
Ehab Masrawa ◽  
...  
Keyword(s):  
Gene Expression ◽  
Gene Transfer ◽  
Cell Lines ◽  
Plasmid Dna ◽  
Mixing Ratio ◽  
Systemic Delivery ◽  
Clear Trend ◽  
Assay Time

A novel cationic polymer, dextran-spermine (D-SPM), has been found to mediate gene expression in a wide variety of cell lines andin vivothrough systemic delivery. Here, we extended the observations by determining the optimal conditions for gene expression of D-SPM/plasmid DNA (D-SPM/pDNA) in cell lines and in the lungs of BALB/c mice via instillation delivery.In vitrostudies showed that D-SPM could partially protect pDNA from degradation by nuclease and exhibited optimal gene transfer efficiency at D-SPM to pDNA weight-mixing ratio of 12. In the lungs of mice, the levels of gene expression generated by D-SPM/pDNA are highly dependent on the weight-mixing ratio of D-SPM to pDNA, amount of pDNA in the complex, and the assay time postdelivery. Readministration of the complex at day 1 following the first dosing showed no significant effect on the retention and duration of gene expression. The study also showed that there was a clear trend of increasing size of the complexes as the amount of pDNA was increased, where the sizes of the D-SPM/pDNA complexes were within the nanometer range.

Tumor Targeting of Plasmid DNA by Dextran Conjugation Based on Metal Coordination

2005 ◽  
Vol 288-289 ◽  
pp. 109-112
Author(s):  
H. Hosseinkhani ◽  
T. Aoyama ◽  
O. Ogawa ◽  
Yasuhiko Tabata
Keyword(s):  
Gene Expression ◽  
Zeta Potential ◽  
Plasmid Dna ◽  
Tumor Targeting ◽  
Metal Coordination ◽  
Hydroxyl Groups ◽  
Negative Zeta Potential

Tumor targeting of plasmid DNA was achieved through the conjugation of dextran derivative with chelate residue based on metal coordination. Spermine (Sm) was chemically introduced to the hydroxyl groups of dextran to obtain dextran-Sm derivative. A negative zeta potential of plasmid DNA became almost 0 mV by the Zn2+-coordinated conjugation with the dextran-Sm When the dextran-Sm-plasmid DNA conjugate with Zn2+ coordination was intravenously injected to mice subcutaneously bearing Meth-AR-1 fibrosarcoma, the dextran- Sm-plasmid DNA conjugate significantly enhanced the level of gene expression in the tumor, in contrast to free plasmid DNA..

An optimized extended DNA kappa B site that enhances plasmid DNA nuclear import and gene expression

2009 ◽  
Vol 11 (5) ◽  
pp. 401-411 ◽  
Author(s):  
Cristine Gonçalves ◽  
Marie-Yvonne Ardourel ◽  
Martine Decoville ◽  
Gilles Breuzard ◽  
Patrick Midoux ◽  
...  
Keyword(s):  
Gene Expression ◽  
Plasmid Dna ◽  
Nuclear Import
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