Overexpression of NDUFV1 alleviates renal damage by improving mitochondrial function in unilateral ureteral obstruction model mice

2021 ◽  
Author(s):  
Ruiting Wang ◽  
Chen Kairen ◽  
Lu Li ◽  
Lingling Zhang ◽  
Haifeng Gong ◽  
...  
Keyword(s):  
Mitochondrial Function ◽  
Ureteral Obstruction ◽  
Renal Damage ◽  
Unilateral Ureteral Obstruction

scholarly journals A novel cell-permeable antioxidant peptide decreases renal tubular apoptosis and damage in unilateral ureteral obstruction

2008 ◽  
Vol 295 (5) ◽  
pp. F1545-F1553 ◽  
Author(s):  
Yasunori Mizuguchi ◽  
Jie Chen ◽  
Surya V. Seshan ◽  
Dix P. Poppas ◽  
Hazel H. Szeto ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
P38 Mapk ◽  
Ureteral Obstruction ◽  
Renal Damage ◽  
Transforming Growth Factor ◽  
Unilateral Ureteral Obstruction ◽  
Heme Oxygenase 1 ◽  
Myocardial Infraction

Unilateral ureteral obstruction (UUO) is characterized by decreases in renal function, increased interstitial fibrosis, tubular apoptosis, and cellular infiltration. It has been suggested that inhibition of tubular apoptosis may protect against renal damage in obstruction. We have recently developed a series of peptides which are concentrated in the inner mitochondrial membrane and prevent cell death. These peptides are also active in vivo, in myocardial infraction, ischemic brain injury, and amyotrophic lateral sclerosis models. We therefore used SS-31, a prototype of these peptides, and assessed its effects on renal damage and oxidative stress in a 14-day obstruction model. SS-31 (1 or 3 mg/kg) or saline was given 1 day before and throughout the 14 days of obstruction. Kidneys were harvested and assessed for apoptosis (terminal transferase-dUTP-nick-end labeling, caspase 3 expression), fibrosis (trichrome staining), macrophage infiltration, fibroblast expression (immunoperoxidase), and oxidative damage (8-OH deoxyguanosine and heme oxygenase-1 expression), cytokines, and signaling pathways (transforming growth factor-β, CCR-1, p38-MAPK, NF-κB). SS-31 significantly attenuated the effects of obstruction on all aspects of renal damage which were examined, with both the 1 and 3 mg/kg doses showing efficacy. We noted increased oxidative stress in obstruction, which was also attenuated by SS-31 treatment. Signaling via NF-κB and p38 MAPK pathways were both affected by SS-31 treatment. This study provides a proof of concept that peptides which protect mitochondria in vitro can provide protection from renal damage in a UUO model. The mechanism by which protection is afforded requires further studies both in vitro and in vivo.

Renal Damage Inhibited in Mice Lacking Angiotensinogen Gene Subjected to Unilateral Ureteral Obstruction

Urology ◽  
2009 ◽  
Vol 74 (4) ◽  
pp. 938-943 ◽  
Author(s):  
Yasumitsu Uchida ◽  
Akira Miyajima ◽  
Eiji Kikuchi ◽  
Norihide Kozakai ◽  
Takeo Kosaka ◽  
...  
Keyword(s):  
Ureteral Obstruction ◽  
Renal Damage ◽  
Unilateral Ureteral Obstruction ◽  
Angiotensinogen Gene

scholarly journals Beneficial effects of Oltipraz, nuclear factor - erythroid – 2 - related factor 2 (Nrf2), on renal damage in unilateral ureteral obstruction rat model

2018 ◽  
Vol 44 (6) ◽  
pp. 1243-1251 ◽  
Author(s):  
Emre Can Polat ◽  
Huseyin Besiroglu ◽  
Levent Ozcan ◽  
Alper Otunctemur ◽  
Ahmet Tugrul Eruyar ◽  
...  
Keyword(s):  
Rat Model ◽  
Ureteral Obstruction ◽  
Renal Damage ◽  
Unilateral Ureteral Obstruction ◽  
Related Factor ◽  
Nuclear Factor ◽  
Beneficial Effects

scholarly journals Dietary arginine supplementation attenuates renal damage after relief of unilateral ureteral obstruction in rats1

2005 ◽  
Vol 68 (2) ◽  
pp. 515-528 ◽  
Author(s):  
Keiichi Ito ◽  
J.I.E. Chen ◽  
Surya V. Seshan ◽  
Jonathan J Khodadadian ◽  
Rachel Gallagher ◽  
...  
Keyword(s):  
Ureteral Obstruction ◽  
Renal Damage ◽  
Unilateral Ureteral Obstruction ◽  
Arginine Supplementation

scholarly journals Pengaruh Valsartan Terhadap Fibrosis Ginjal pada Obstruksi Ureter

2012 ◽  
Vol 1 (2) ◽  
Author(s):  
Alvarino Alvarino
Keyword(s):  
Treatment Group ◽  
Ureteral Obstruction ◽  
Wistar Rats ◽  
Renal Damage ◽  
Interstitial Fibrosis ◽  
Unilateral Ureteral Obstruction ◽  
Tubular Atrophy ◽  
Left Ureter ◽  
Significant Difference ◽  
Valsartan Group

AbstrakPendahuluan : Obstruksi ureter merupakan kondisi yang dapat terjadi pada setiap usia dengan levelbervariasi dengan efek terburuk berupa gagal ginjal permanen. Terdapat berbagai mekanisme yang berperandalam terjadinya kerusakan ginjal pada obstruksi ureter. Penelitian ini bertujuan untuk mengamati pengaruhvalsartan untuk memperbaiki kerusakan ginjal pada kondisi tersebut. Metode : Dilakukan penelitian eksperimentalmenggunakan 30 ekor tikus wistar dengan obstruksi ureter unilateral melalui pengikatan ureter kiri yang dibagimenjadi dua kelompok, tanpa pemberian valsartan dan dengan pemberian valsartan. Pada hari ke-14 dinilai dandibandingkan fibrosis interstisial, degenerasi hidrofik, dan atrofi tubulus pada kedua kelompok dengan pulasanhematoksilin-eosin. Hanya tikus yang tetap hidup hingga melewati hari ketujuh yang dimasukkan dalam penelitian.Hasil : Dari tiga belas tikus wistar pada kelompok obstruksi ureter unilateral tanpa pemberian valsartan, didapatkan11 tikus mengalami fibrosis interstisial pada ringan dan 2 tikus mengalami fibrosis interstisial sedang, sementaraseluruh tikus wistar pada kelompok dengan valsartan mengalami fibrosis interstisial ringan (p > 0.05). Tidakterdapat perbedaan bermakna untuk degenerasi hidrofik epitel tubulus antara kelompok tanpa valsartan dandengan valsartan (31.46 vs 33.67, p > 0.05). Tidak terdapat pula perbedaan bermakna untuk atrofi tubuli antarakedua kelompok (61.78 vs 62.07, p > 0.05). Kesimpulan : Pemberian valsartan dengan dosis antihipertensi tidakmengurangi tingkat fibrosis interstisial, degenerasi hidrofik, maupun atrofi tubulus pada obstruksi ureter unilateralpada tikus wistarKata kunci: obstruksi ureter unilateral, valsartan, fibrosis interstisialAbstractIntroduction: Ureteral obstruction is a condition that might happen at any age in any level with the worstcomplication is permanent renal damage. There are several mechanisms that play role in renal damage in ureteralobstruction. This experimental research is aimed to observe the effect of valsartan to attenuate renal damage inunilateral ureteral obstruction. MethodsExperimental research was performed using 30 wistar rats with unilateralureteral obstruction achieved by ligation of the left ureter. Rats then divided into two groups, no treatment groupand valsartan group. At the 14th day, evaluation was performed to compare interstitial fibrosis, hydrophilicdegeneration, and tubular atrophy between the two groups using haematoxylin-eosin staining. Only rats surviveuntil at least 7th day are included in the study. ResultFrom thirteen wistar rats in no treatment group, two of themhaving moderate interstitial fibrosis and eleven with mild interstitial fibrosis while all rats in valsartan group had mildinterstitial fibrosis (p > 0.05). There is no significant difference on hydrophilic degeneration between no treatmentand valsartan group (31.46 vs 33.67, p > 0.05). There is also no significant difference in tubular atrophy betweenthe two groups (61.78 vs 62.07, p > 0.05). ConclusionValsartan therapy in antihypertensive dosage has nosignificant effect in to attenuate interstitial fibrosis, hydrophilic degeneration, and tubular atrophy in unilateralureteral obstruction in wistar rats.Keywords: unilateral ureteral obstruction, valsartan, interstitial fibrosis

scholarly journals Pomegranate extract attenuates unilateral ureteral obstruction-induced renal damage by reducing oxidative stress

2015 ◽  
Vol 7 (2) ◽  
pp. 166 ◽  
Author(s):  
Alper Otunctemur ◽  
Emin Ozbek ◽  
SuleymanSami Cakir ◽  
EmreCan Polat ◽  
Murat Dursun ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Ureteral Obstruction ◽  
Renal Damage ◽  
Unilateral Ureteral Obstruction

Thymoquinone ameliorates renal damage in unilateral ureteral obstruction in rats

2017 ◽  
Vol 69 (4) ◽  
pp. 648-657 ◽  
Author(s):  
Sara Hosseinian ◽  
Abolfazl Khajavi Rad ◽  
Alireza Ebrahimzadeh Bideskan ◽  
Mohammad Soukhtanloo ◽  
Hamidreza Sadeghnia ◽  
...  
Keyword(s):  
Ureteral Obstruction ◽  
Renal Damage ◽  
Unilateral Ureteral Obstruction

Renal damage progresses despite improvement of renal function after relief of unilateral ureteral obstruction in adult rats

2004 ◽  
Vol 287 (6) ◽  
pp. F1283-F1293 ◽  
Author(s):  
Keiichi Ito ◽  
Jie Chen ◽  
Maher El Chaar ◽  
Joshua M. Stern ◽  
Surya V. Seshan ◽  
...  
Keyword(s):  
Renal Function ◽  
Ureteral Obstruction ◽  
Renal Damage ◽  
Interstitial Fibrosis ◽  
Unilateral Ureteral Obstruction ◽  
Pharmacological Intervention ◽  
Adult Rats ◽  
Group 5 ◽  
Group 1

Progression of renal damage after relief of unilateral ureteral obstruction (UUO) has been demonstrated, especially in neonatal rats. We evaluated renal function and renal damage after relief of 3-day UUO in five groups of adult rats: group 1, no treatment; group 2, 3-day UUO; groups 3- 5, 3-day UUO followed by relief; group 3, 7-day relief; group 4, 14-day relief; and group 5, 28-day relief. Glomerular filtration rate (GFR), renal blood flow (RBF), tissue transforming growth factor-β (TGF-β), interstitial fibrosis and fibroblast expression, tubular apoptosis, macrophage infiltration, expression of nitric oxide synthases (NOS), and urinary nitrate/nitrite (NO2/NO3) were evaluated. RBF and GFR were decreased to <10% of baseline by 3 days of UUO. GFR and RBF in a previously obstructed kidney (POK) returned to baseline by 14 days after relief. Both tissue TGF-β1 and interstitial fibrosis were significantly higher in POK of groups 3- 5 compared with groups 1 and 2 . In group 5, the numbers of infiltrating macrophages, fibroblasts, and apoptotic tubular cells were higher in POK compared with group 1. Urinary NO2/NO3 was significantly higher than baseline from 3 to 27 days after relief of UUO. Expression of NOS isoforms was increased in tubules. As interstitial fibrosis contributes to decreased renal function, these results suggest that the acute recovery in function may be compromised in the long term by the progressive renal fibrosis which was found. Furthermore, pharmacological intervention at the time of relief of UUO, targeted to fibrotic processes, may contribute to long-term recovery of renal function.

scholarly journals Unilateral Ureteral Obstruction for 28 Days in Rats Is Not Associated with Changes in Cardiac Function or Alterations in Mitochondrial Function

Biology ◽  
2021 ◽  
Vol 10 (7) ◽  
pp. 671
Author(s):  
Rodrigo Prieto-Carrasco ◽  
Alejandro Silva-Palacios ◽  
Pedro Rojas-Morales ◽  
Omar Emiliano Aparicio-Trejo ◽  
Estefany Ingrid Medina-Reyes ◽  
...  
Keyword(s):  
Antioxidant Enzymes ◽  
Cardiac Function ◽  
Western Blot ◽  
Mitochondrial Function ◽  
Ureteral Obstruction ◽  
Renal Damage ◽  
Histological Analysis ◽  
Cardiac Mitochondria ◽  
Cardiac Damage ◽  
Male Wistar Rats

Our work evaluated cardiac function and mitochondrial bioenergetics parameters in hearts from male Wistar rats subjected to the UUO model during 28 days of progression. We measured markers of kidney damage and inflammation in plasma and renal fibrosis by histological analysis and Western blot. Cardiac function was evaluated by echocardiography and proteins involved in cardiac damage by Western blot. Oxygen consumption and transmembrane potential were monitored in cardiac mitochondria using high-resolution respirometry. We also determined the activity of ATP synthase and antioxidant enzymes such as glutathione peroxidase, glutathione reductase, and catalase. Our results show that, although renal dysfunction is established in animals subjected to ureteral obstruction, cardiac function is maintained along with mitochondrial function and antioxidant enzymes activity after 28 days of injury evolution. Our results suggest that renocardiac syndrome might develop but belatedly in obstruction-induced renal damage, opening the opportunity for treatment to prevent this condition.

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