Ex vivo and in vivo Evaluation of [18F]PR04.MZ in Rodents: A Selective Dopamine Transporter Imaging Agent

ChemMedChem ◽  
2009 ◽  
Vol 4 (9) ◽  
pp. 1480-1487 ◽  
Author(s):  
Patrick J. Riss ◽  
Fabian Debus ◽  
René Hummerich ◽  
Ulrich Schmidt ◽  
Patrick Schloss ◽  
...  
Keyword(s):  
Dopamine Transporter ◽  
Ex Vivo ◽  
Imaging Agent ◽  
In Vivo Evaluation ◽  
Dopamine Transporter Imaging ◽  
Selective Dopamine

scholarly journals Synthesis, Radiolabeling, and Preliminary in vivo Evaluation of [68Ga] IPCAT-NOTA as an Imaging Agent for Dopamine Transporter

2021 ◽  
Vol Volume 15 ◽  
pp. 2577-2591
Author(s):  
Shiou-Shiow Farn ◽  
Kang-Wei Chang ◽  
Wan-Chi Lin ◽  
Hung-Man Yu ◽  
Kun-Liang Lin ◽  
...  
Keyword(s):  
Dopamine Transporter ◽  
Imaging Agent ◽  
In Vivo Evaluation

Synthesis and characterization of Chitosan-Catechol conjugates: Development and in vitro, in silico and in vivo evaluation of mucoadhesive pellets of lafutidine

2021 ◽  
pp. 088391152199784
Author(s):  
Loveleen Kaur ◽  
Ajay Kumar Thakur ◽  
Pradeep Kumar ◽  
Inderbir Singh
Keyword(s):  
Drug Release ◽  
In Silico ◽  
Ex Vivo ◽  
Strong Interactions ◽  
Dissolution Time ◽  
Sem Images ◽  
In Vivo Evaluation ◽  
Release Studies

Present study was aimed to synthesize and characterize Chitosan-Catechol conjugates and to design and develop mucoadhesive pellets loaded with lafutidine. SEM images indicated the presence of fibrous structures responsible for enhanced mucoadhesive potential of Chitosan-Catechol conjugates. Thermodynamic stability and amorphous nature of conjugates was confirmed by DSC and XRD studies respectively. Rheological studies were used to evaluate polymer mucin interactions wherein strong interactions between Chitosan-Catechol conjugate and mucin was observed in comparison to pristine chitosan and mucin. The mucoadhesion potential of Chitosan-Catechol (Cht-C) versus Chitosan (Cht) was assessed in silico using molecular mechanics simulations and the results obtained were compared with the in vitro and ex vivo results. Cht-C/mucin demonstrated much higher energy stabilization (∆E ≈ −65 kcal/mol) as compared to Cht/mucin molecular complex. Lafutidine-loaded pellets were prepared from Chitosan (LPC) and Chitosan-Catechol conjugates (LPCC) and were evaluated for various physical properties viz. flow, circularity, roundness, friability, drug content, particle size and percent mucoadhesion. In vitro drug release studies on LPC and LPCC pellets were performed for computing t50%, t90% and mean dissolution time. The values of release exponent from Korsmeyer-Peppas model was reported to be 0.443 and 0.759 for LPC and LPCC pellets suggesting Fickian and non-Fickian mechanism representing drug release, respectively. In vivo results depicted significant controlled release and enhanced residence of the drug after being released from the chitosan-catechol coated pellets. Chitosan-Catechol conjugates were found to be a promising biooadhesive polymer for the development of various mucoadhesive formulations.

scholarly journals Effect of Processing on Fish Protein Antigenicity and Allergenicity

Foods ◽  
2021 ◽  
Vol 10 (5) ◽  
pp. 969
Author(s):  
Xingyi Jiang ◽  
Qinchun Rao
Keyword(s):  
Fish Species ◽  
Protein Denaturation ◽  
Ex Vivo ◽  
Protein Solubility ◽  
Future Research ◽  
Food Protein ◽  
In Vivo Evaluation ◽  
Fish Allergy

Fish allergy is a life-long food allergy whose prevalence is affected by many demographic factors. Currently, there is no cure for fish allergy, which can only be managed by strict avoidance of fish in the diet. According to the WHO/IUIS Allergen Nomenclature Sub-Committee, 12 fish proteins are recognized as allergens. Different processing (thermal and non-thermal) techniques are applied to fish and fishery products to reduce microorganisms, extend shelf life, and alter organoleptic/nutritional properties. In this concise review, the development of a consistent terminology for studying food protein immunogenicity, antigenicity, and allergenicity is proposed. It also summarizes that food processing may lead to a decrease, no change, or even increase in fish antigenicity and allergenicity due to the change of protein solubility, protein denaturation, and the modification of linear or conformational epitopes. Recent studies investigated the effect of processing on fish antigenicity/allergenicity and were mainly conducted on commonly consumed fish species and major fish allergens using in vitro methods. Future research areas such as novel fish species/allergens and ex vivo/in vivo evaluation methods would convey a comprehensive view of the relationship between processing and fish allergy.

scholarly journals Pivotal role of Acitretin nanovesicular gel for effective treatment of psoriasis: ex vivo–in vivo evaluation study

2018 ◽  
Vol Volume 13 ◽  
pp. 1059-1079 ◽  
Author(s):  
Irhan Abu Hashim ◽  
Noha Abo El-Magd ◽  
Ahmed El-Sheakh ◽  
Mohammed Hamed ◽  
Abd El-Gawad Abd El-Gawad
Keyword(s):  
Effective Treatment ◽  
Ex Vivo ◽  
Evaluation Study ◽  
Pivotal Role ◽  
In Vivo Evaluation

Synthesis, radiolabeling and preliminary in vivo evaluation of [18F]FE-PE2I, a new probe for the dopamine transporter

2009 ◽  
Vol 19 (16) ◽  
pp. 4843-4845 ◽  
Author(s):  
Magnus Schou ◽  
Carsten Steiger ◽  
Andrea Varrone ◽  
Denis Guilloteau ◽  
Christer Halldin
Keyword(s):  
Dopamine Transporter ◽  
In Vivo Evaluation

scholarly journals In Vitro and In Vivo Evaluation of Human Adenovirus Type 49 as a Vector for Therapeutic Applications

Viruses ◽  
2021 ◽  
Vol 13 (8) ◽  
pp. 1483
Author(s):  
Emily A. Bates ◽  
John R. Counsell ◽  
Sophie Alizert ◽  
Alexander T. Baker ◽  
Natalie Suff ◽  
...  
Keyword(s):  
Viral Vector ◽  
Ex Vivo ◽  
Human Adenovirus ◽  
Adenovirus Type ◽  
Cell Types ◽  
In Vivo Evaluation ◽  
In Vivo Biodistribution ◽  
Adenovirus Type 5

The human adenovirus phylogenetic tree is split across seven species (A–G). Species D adenoviruses offer potential advantages for gene therapy applications, with low rates of pre-existing immunity detected across screened populations. However, many aspects of the basic virology of species D—such as their cellular tropism, receptor usage, and in vivo biodistribution profile—remain unknown. Here, we have characterized human adenovirus type 49 (HAdV-D49)—a relatively understudied species D member. We report that HAdV-D49 does not appear to use a single pathway to gain cell entry, but appears able to interact with various surface molecules for entry. As such, HAdV-D49 can transduce a broad range of cell types in vitro, with variable engagement of blood coagulation FX. Interestingly, when comparing in vivo biodistribution to adenovirus type 5, HAdV-D49 vectors show reduced liver targeting, whilst maintaining transduction of lung and spleen. Overall, this presents HAdV-D49 as a robust viral vector platform for ex vivo manipulation of human cells, and for in vivo applications where the therapeutic goal is to target the lung or gain access to immune cells in the spleen, whilst avoiding liver interactions, such as intravascular vaccine applications.

Ex Vivo and in Vivo Evaluation of the Effect of Coating a Coumarin-6-Labeled Nanostructured Lipid Carrier with Chitosan-N-acetylcysteine on Rabbit Ocular Distribution

2017 ◽  
Vol 14 (8) ◽  
pp. 2639-2648 ◽  
Author(s):  
Dandan Liu ◽  
Jinyu Li ◽  
Bingchao Cheng ◽  
Qingyin Wu ◽  
Hao Pan
Keyword(s):  
Ex Vivo ◽  
Nanostructured Lipid Carrier ◽  
In Vivo Evaluation ◽  
Ocular Distribution ◽  
Coumarin 6

Synthesis and in vivo evaluation of [18F]N-(2-benzofuranylmethyl)-N′-[4-(2-fluoroethoxy)benzyl]piperazine, a novel σ1 receptor PET imaging agent

2011 ◽  
Vol 21 (22) ◽  
pp. 6820-6823 ◽  
Author(s):  
Iman A. Moussa ◽  
Samuel D. Banister ◽  
Nicolas Giboureau ◽  
Steven R. Meikle ◽  
Michael Kassiou
Keyword(s):  
Pet Imaging ◽  
Imaging Agent ◽  
In Vivo Evaluation ◽  
Σ1 Receptor

[99mTc]TRODAT-1: A novel technetium-99m complex as a dopamine transporter imaging agent

1997 ◽  
Vol 24 (4) ◽  
pp. 372-380 ◽  
Author(s):  
Mei-Ping Kung ◽  
D. Andrew Stevenson ◽  
Karl Pl�ssl ◽  
Sanath K. Meegalla ◽  
Andrew Beckwith ◽  
...  
Keyword(s):  
Dopamine Transporter ◽  
Imaging Agent ◽  
Dopamine Transporter Imaging ◽  
Technetium 99M
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